Hi Arek,

 

Thanks for looking into this for me.

 

Regards,

Stan

 

From: Arek Kasprzyk [mailto:[email protected]] 
Sent: June-21-12 10:44 AM
To: Stanley Wai-Kwong Ng
Cc: [email protected]; Robin Haw
Subject: Re: Stanley Ng - R Biomart Reactome Query Error

 

Hi Stan,

this looks like misconfiguration on the Reactome side. I am forwarding your
email to the Reactome mart administrators who will be able to help with that

 

 

a

On Thu, Jun 21, 2012 at 3:08 PM, Stanley Wai-Kwong Ng
<[email protected]> wrote:

Hi Arek,

 

I am a PhD student at the University of Toronto and am using biomart in R to
get a list of entrez gene Ids for some reactome pathways in humans.

 

I ran the following query last month and it worked with no problems:

 

library("biomaRt")

mart = useMart("REACTOME", dataset="pathway")

pathwayids =
getBM(attributes=c("stableidentifier_identifier"),filters="species_selection
",values=c("Homo sapiens"), mart=mart)

pathwayids = c(do.call("cbind",pathwayids))

 
entrezids =
getBM(attributes=c("referencedatabase_entrez_gene"),filters="pathway_id_list
",values=c(pathwayids[i]), mart=mart)

 

However, I tried to run the commands again last night and today and I get
the following error:

 

Error in getBM(attributes = c("referencedatabase_entrez_gene"), filters =
"pathway_id_list",  : 

  Query ERROR: caught BioMart::Exception::Query: <pre>Query error occurred
at web service based data source!

Remote data source: http://www.reactome.org:5555/biomart/martservice?

Query XML:

&lt;?xml version="1.0" encoding="UTF-8"?&gt;

&lt;!DOCTYPE Query&gt;

&lt;Query  virtualSchemaName = "default" limitStart = "" limitSize = "200"
count = "" softwareVersion = "0.6" requestId= "biomart-client"&gt;

        &lt;Dataset name = "pathway" interface = "default" &gt;

                                &lt;Attribute name =
"referencedatabase_entrez_gene" /&gt;

                &lt;ValueFilter name = "pathway_id_list" value =
"REACT_1698"/&gt;

          &lt;/Dataset&gt;

&lt;/Query&gt;

Please ensure the above query XML is well-formed and does not contain
illegal characters.

Error message from remote server as below:

Query ERROR: caught BioMart::Exception::Database: Error during query
execution: Unknown column
'test_reactome_mart.pathway__referencednasequence__dm.referencedatabase_entr
ez_gene' in 'field list'

</pre>

 

Can you advise on what might be wrong?

 

Thanks,

Stan





 

-- 
Arek Kasprzyk, MD, MSc, PhD 
BioMart Project Lead
www.biomart.org

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