Understood, clarified resample is the solution. Thanks,
BTW, when I read the txrx_loopback_to_file example, it does make 2 usrp objects 
(1 tx, 1 rx) point to 1 device, how can it work?

From: Marcus D. Leech <[email protected]>
Sent: Saturday, May 7, 2022 9:50 PM
To: [email protected]
Subject: [USRP-users] Re: X300 simultaneously transmit 2 signals of different 
sample rate and center frequency

On 2022-05-07 08:22, ?? WANG Cui wrote:
Hi,
I searched around and still can’t get clear answer, so I would seek help here.
I want to simultaneously transmit 2 baseband files which are different sample 
rate (one is rate 2.5 MHz at freq 1.5 GHz, and the other is rate 8 MHz at freq 
1.8 GHz), and would like to utilize 2 daughter boards of X300.
I tried to configure it with 2 multi_usrp objects, but the call failed when get 
second tx_streamer (however, the first get call succeed.), with: Error: 
RuntimeError: Multiple sampling rates downstream of TX terminator 0: ...
I know X300 can transmit/receive 2 channels at 2 different sample rate, just 
wonder is it possible to simultaneously transmit 2 channels as described above? 
Or have to resample one file to the same sample rate of the other file, then 
transmit together?
Thanks in advance,
WANG Cui

You can't have multiple multi_usrp objects pointing to the same device, so 
you'll have to resample one of your streams and use a
  single multi_usrp object.


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