Daniel,   I think that the clue here is '(it's usually the first one in that
day, or when I try to log on during the weekend)'

DAO, and ADO and any method of accessing an MDB that I know off requires
that the first person to access the database has create access to the folder
in which the database resides.  This is so that the locking (LDB) file can
be written.

I would suggest that the account being used to open the database connection
does not have create access to this folder.

You say that a restart of ISS fixes the problem, but is that because ISS has
a problem, or a different security access is being used to open the file
after the ISS restart.

Matt



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