Hi superZZ,

 you can first extract a surface

vmtkmarchingcubes -ifile blah.mha -l 0.5 -ofile blah.vtp

or eventually smooth it

vmtkmarchingcubes -ifile blah.mha -l 0.5 --pipe vmtksurfacesmoothing -passband 
0.1 -iterations 30 -ofile blah.vtp

and then create one opening on the surface somewhere (at the root of the tree, 
assuming there is one?)

vmtksurfaceclipper -ifile blah.vtp -ofile blah_open.vtp

and extract a surface

vmtknetworkextraction -ifile blah_open.vtp -ofile blah_network.vtp

This uses an algorithm that has low complexity, it can handle complicated 
networks but it can also
generate centerlines that exit the initial surface (it is not extremely 
accurate). How accurate depends
on your application.

The accurate alternative is to use vmtkcenterlines. For this you either have to 
open the surface at all endpoints
and use the -seedselector openprofiles option, or to place seeds on all 
endpoints.

vmtkcenterlines -ifile blah.vtp -ofile blah_centerlines.vtp


Hope this helps


Luca



On Apr 6, 2012, at 9:45 AM, Lingfeng Zhuang wrote:

> Dear Luca,
> 
> Does VMTK support automatically extract all centerlines from a 3D segmented 
> binary image? This image has no islet.
> 
> Regards,
> 
> superZZ
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