2013/7/23  <[email protected]>:
> Another example which seems to be correct but wish to know, please see
> attached. The derivative (last column) of function H(x) is zero while H(x)
> is highly positive... Is that correct potential?
I don't see a problem here, the begin of the potential is constant and
hence the derivative is zero.

> My protein still stays in a
> globular conformation and I corrected other potentials. If it is ok would
> you try to make IBI with potentials one by one?
do_potential in the xml file allows you to divide interactions into
group to update only one group at the time.
The default will put all interactions in one group.

I would approximate your 4 special interactions by a non-linear
spring-like potential (see my earlier email) and keep them fixed for
now and do IBI on the other 11 interactions.


>
> Steven
>
> W dniu wtorek, 23 lipca 2013 10:22:26 UTC+1 użytkownik [email protected]
> napisał:
>>
>> Could you please also explain me why from attached potential Votca produce
>> a table with negative (close to zero) values at low distances? It does not
>> make sense and hence my protein remains globular... I have 4 potentials like
>> this out of 15...
>>
>> Steven
>>
>> W dniu poniedziałek, 22 lipca 2013 23:40:50 UTC+1 użytkownik Christoph
>> Junghans napisał:
>>>
>>> 2013/7/22  <[email protected]>:
>>> >
>>> >
>>> > On Friday, July 19, 2013 8:02:01 PM UTC+1, Christoph Junghans wrote:
>>> >>
>>> >> 2013/7/19  <[email protected]>:
>>> >> > Thank you for this.
>>> >> > I recalculated my distributions and make them a value between 0 and
>>> >> > 1 at
>>> >> > large distances. If I submit then my own potential pot.in which goes
>>> >> > to
>>> >> > a
>>> >> > positive value in this case would votca not shift the potential
>>> >> > after
>>> >> > each
>>> >> > iteration to zero? Please, let me know.
>>> >> VOTCA always shifts the potential, to U(r_max)=0 for non-bonded
>>> >> interaction and U_min=0 for bonded interactions.
>>> >> This is why I said making VOTCA believe your interaction is bonded,
>>> >> might
>>> >> help.
>>> >> The update rule is the same for bonded and non-bonded.
>>> >>
>>> >> Anyhow the shift doesn't change the physics, so why are you so bother
>>> >> about it? You could just to do IBI and shift the finial outcome once
>>> >> it is converged.
>>> >>
>>> >> Christoph
>>> >
>>> >
>>> > Thank you. This what I will do - shift the final potential. what would
>>> > be
>>> > the best cut off for IBI - is that the place where distributions have
>>> > maxima?
>>> That is a tricky question, to be honest I don't know. You might have
>>> to play with the cutoff a bit.
>>>
>>> >
>>> >
>>> > Steven
>>> >
>>> >
>>> >>
>>> >> >
>>> >> > Steven
>>> >> >
>>> >> > W dniu środa, 17 lipca 2013 17:43:51 UTC+1 użytkownik Christoph
>>> >> > Junghans
>>> >> > napisał:
>>> >> >>
>>> >> >> 2013/7/17  <[email protected]>:
>>> >> >> > Thank you for this.
>>> >> >> >
>>> >> >> > Another question - presumably I will specify my own distributions
>>> >> >> > (not
>>> >> >> > from
>>> >> >> > VOTCA) with some simplifications. Then I will create intial
>>> >> >> > potentials
>>> >> >> > to
>>> >> >> > IBI as name.pot.in. Votca will try to fit to my distributions to
>>> >> >> > the
>>> >> >> > one
>>> >> >> > submitted in the main directory. However, by calculating
>>> >> >> > distributions
>>> >> >> > after
>>> >> >> > each step these will not be distributions which could possibly
>>> >> >> > match
>>> >> >> > my
>>> >> >> > initial with simpifications, am I right? Do I have to calculate
>>> >> >> > RDFs
>>> >> >> > at
>>> >> >> > each
>>> >> >> > step on my own then?
>>> >> >> 1.) If you give VOTCA a pot.in, it will not do use the potential of
>>> >> >> mean as initial guess in step_000, but just your potential.
>>> >> >> 2.) Independently of pot.in, VOTCA will always compare dist.new
>>> >> >> against dist.tgt every step
>>> >> >> 3.) dist.new is calculated in every iteration using csg_stat, you
>>> >> >> could make VOTCA fit only the part 0 to min.
>>> >> >> 3b.) You could write a custom post-update script to do whatever rdf
>>> >> >> calculation/ potential modification after you want. This can be
>>> >> >> used
>>> >> >> to overwrite rdf calculated by csg_stat.
>>> >> >> 3c.) All of this seems a bit hacky, why don't you just declare some
>>> >> >> interaction of type bonded, so csg_stat will calculate
>>> >> >> p(r)=H(r)/(4pi*r^2) instead of the rdf for those.
>>> >> >>
>>> >> >> > Another issue: How Votca normalize RDFs using csg_stat ? I wish
>>> >> >> > to
>>> >> >> > have
>>> >> >> > volume and mass normalized distributions, is that possible?
>>> >> >> No, we don't have an option to calculate the  volume and mass
>>> >> >> normalized distributions instead. Though it is not hard to
>>> >> >> implement!
>>> >> >> For IBI that would also make no difference as all normalization
>>> >> >> factors drop out in the ratio g(r)/g_target(r) used in the update.
>>> >> >>
>>> >> >> Christoph
>>> >> >>
>>> >> >>
>>> >> >> >
>>> >> >> > Steven
>>> >> >> >
>>> >> >> > W dniu wtorek, 16 lipca 2013 23:17:05 UTC+1 użytkownik Christoph
>>> >> >> > Junghans
>>> >> >> > napisał:
>>> >> >> >>
>>> >> >> >> 2013/7/16  <[email protected]>:
>>> >> >> >> >
>>> >> >> >> >
>>> >> >> >> > W dniu wtorek, 16 lipca 2013 08:48:51 UTC+1 użytkownik Tristan
>>> >> >> >> > Bereau
>>> >> >> >> > napisał:
>>> >> >> >> >>
>>> >> >> >> >> That sounds like what I was hinting at: from what I
>>> >> >> >> >> understand,
>>> >> >> >> >> you're
>>> >> >> >> >> simulating a single protein, not a pure liquid of stuff. So
>>> >> >> >> >> your
>>> >> >> >> >> RDF
>>> >> >> >> >> will never go to 1 because there won't be anything at large
>>> >> >> >> >> distances.
>>> >> >> >> >> Is that the case? If so, more iterations and/or better
>>> >> >> >> >> initial
>>> >> >> >> >> guesses
>>> >> >> >> >> won't cut it.
>>> >> >> >> >
>>> >> >> >> >
>>> >> >> >> > Yes, this is the case. I will aproximate the RDFs so that they
>>> >> >> >> > go
>>> >> >> >> > to
>>> >> >> >> > sth
>>> >> >> >> > which is non zero. Thank you.
>>> >> >> >> non zero will not be enough, it has to be 1 otherwise your
>>> >> >> >> potential
>>> >> >> >> will still accumulated whatever the value, kT*log(P(r_cut), at
>>> >> >> >> the
>>> >> >> >> cutoff is.
>>> >> >> >>
>>> >> >> >> For me the distributions looks more like a something, which
>>> >> >> >> could be
>>> >> >> >> modeled with a non-linear spring type potential (r-> +/-inf
>>> >> >> >> P->inf),
>>> >> >> >> where the minimum is a zero.
>>> >> >> >> VOTCA could do that for you if declare the interaction as
>>> >> >> >> bonded.
>>> >> >> >> (VOTCA's definition of non-bonded and bonded might not be taken
>>> >> >> >> too
>>> >> >> >> strict.)
>>> >> >> >> Also from the modeling point of view, it might make sense to
>>> >> >> >> have a
>>> >> >> >> spring between some beads, which cannot go infinitely apart due
>>> >> >> >> to
>>> >> >> >> geometry.
>>> >> >> >>
>>> >> >> >> Anyhow, these are scientific decisions you have to make
>>> >> >> >> yourself.
>>> >> >> >>
>>> >> >> >> Christoph
>>> >> >> >>
>>> >> >> >> >
>>> >> >> >> > Steven
>>> >> >> >> >>
>>> >> >> >> >>
>>> >> >> >> >> On Mon, Jul 15, 2013 at 10:09 PM,  <[email protected]>
>>> >> >> >> >> wrote:
>>> >> >> >> >> > Thank you for this. For heterogenous system RDF does not go
>>> >> >> >> >> > to
>>> >> >> >> >> > 1
>>> >> >> >> >> > but
>>> >> >> >> >> > to
>>> >> >> >> >> > 0.
>>> >> >> >> >> > In this case I guess I need thousands of iterations... The
>>> >> >> >> >> > system
>>> >> >> >> >> > input
>>> >> >> >> >> > are
>>> >> >> >> >> > 15 potentials which makes it so complicated.
>>> >> >> >> >> >
>>> >> >> >> >> > Steven
>>> >> >> >> >> >
>>> >> >> >> >> > W dniu poniedziałek, 15 lipca 2013 17:44:46 UTC+1
>>> >> >> >> >> > użytkownik
>>> >> >> >> >> > Christoph
>>> >> >> >> >> > Junghans napisał:
>>> >> >> >> >> >>
>>> >> >> >> >> >> 2013/7/15  <[email protected]>:
>>> >> >> >> >> >> > Votca is definitely wrong. If you take the example of
>>> >> >> >> >> >> > maximum
>>> >> >> >> >> >> > of
>>> >> >> >> >> >> > my
>>> >> >> >> >> >> > ACI-ACI.dist.tgt the maximum corresponds to 65.555. The
>>> >> >> >> >> >> > potential
>>> >> >> >> >> >> > at
>>> >> >> >> >> >> > this
>>> >> >> >> >> >> > point should be: W = -2.49435*ln(65.55) = -10.433 and in
>>> >> >> >> >> >> > my
>>> >> >> >> >> >> > ACI-ACI.dist.pot
>>> >> >> >> >> >> > the value corresponds to -16.1 - it is a huge difference
>>> >> >> >> >> >> > and
>>> >> >> >> >> >> > that
>>> >> >> >> >> >> > is
>>> >> >> >> >> >> > why
>>> >> >> >> >> >> > my
>>> >> >> >> >> >> > further distributions are so huge....
>>> >> >> >> >> >> No, Votca is 100% correct, and does what it is supposed to
>>> >> >> >> >> >> do.
>>> >> >> >> >> >>
>>> >> >> >> >> >> First, have a look at your ACI-ACI.dist.tgt again, this
>>> >> >> >> >> >> distribution
>>> >> >> >> >> >> doesn't go to one hence the potential doesn't go to 0 for
>>> >> >> >> >> >> large
>>> >> >> >> >> >> r.
>>> >> >> >> >> >> And that is mainly the reason why VOTCA cannot handle it,
>>> >> >> >> >> >> ACI-ACI.dist.tgt is not a common rdf!
>>> >> >> >> >> >> You will have to provide an initial guess (pot.in) to make
>>> >> >> >> >> >> it
>>> >> >> >> >> >> work.
>>> >> >> >> >> >> (Please also read my email from July 10th again.)
>>> >> >> >> >> >>
>>> >> >> >> >> >> Second, VOTCA does exactly what it is supposed to do. Go
>>> >> >> >> >> >> into
>>> >> >> >> >> >> gnuplot
>>> >> >> >> >> >> and
>>> >> >> >> >> >> run:
>>> >> >> >> >> >> p [0:3][-20:5] "ACI-ACI.dist.tgt" u
>>> >> >> >> >> >> 1:(-2.49435*log($2)-5.7) w
>>> >> >> >> >> >> l,
>>> >> >> >> >> >> "ACI-ACI.pot.cur" w l
>>> >> >> >> >> >> Except for some small deviations, which come from the
>>> >> >> >> >> >> cubic
>>> >> >> >> >> >> spline
>>> >> >> >> >> >> interpolation, there is no difference in the curves.
>>> >> >> >> >> >> As Victor said before, VOTCA shifts the potential to be
>>> >> >> >> >> >> zero
>>> >> >> >> >> >> at
>>> >> >> >> >> >> the
>>> >> >> >> >> >> cutoff -> -10.433 - 5.7 = -16.1. This shift of 5.7 makes
>>> >> >> >> >> >> no
>>> >> >> >> >> >> difference
>>> >> >> >> >> >> for the thermodynamics however.
>>> >> >> >> >> >>
>>> >> >> >> >> >> Third, even pot.new is correct. Run
>>> >> >> >> >> >> $ paste ACI-ACI.dist.new <(sed '/^#/d' ACI-ACI.dist.tgt)
>>> >> >> >> >> >> <(sed
>>> >> >> >> >> >> '/^#/d'
>>> >> >> >> >> >> ACI-ACI.pot.cur) > ACI-ACI.temp
>>> >> >> >> >> >> to generate a temp file.
>>> >> >> >> >> >> And go into gnuplot and plot:
>>> >> >> >> >> >> p [0:3][-20:5] "ACI-ACI.temp" u
>>> >> >> >> >> >> 1:(2.49435*log($2/$5)+$8-16.1)
>>> >> >> >> >> >> w
>>> >> >> >> >> >> l,
>>> >> >> >> >> >> "ACI-ACI.pot.new" w l
>>> >> >> >> >> >> There is basically no difference in the curves.
>>> >> >> >> >> >>
>>> >> >> >> >> >> Conclusion:
>>> >> >> >> >> >> - check your distributions again
>>> >> >> >> >> >> - provide pot.in for the interaction, which don't have a
>>> >> >> >> >> >> "common"
>>> >> >> >> >> >> rdf
>>> >> >> >> >> >> (meaning which doesn't go to 1)
>>> >> >> >> >> >>
>>> >> >> >> >> >> Christoph
>>> >> >> >> >> >>
>>> >> >> >> >> >>
>>> >> >> >> >> >> >
>>> >> >> >> >> >> >
>>> >> >> >> >> >> >
>>> >> >> >> >> >> >
>>> >> >> >> >> >> > W dniu poniedziałek, 15 lipca 2013 12:59:40 UTC+1
>>> >> >> >> >> >> > użytkownik
>>> >> >> >> >> >> > [email protected] napisał:
>>> >> >> >> >> >> >>
>>> >> >> >> >> >> >>
>>> >> >> >> >> >> >>
>>> >> >> >> >> >> >> W dniu poniedziałek, 15 lipca 2013 12:42:37 UTC+1
>>> >> >> >> >> >> >> użytkownik
>>> >> >> >> >> >> >> Victor
>>> >> >> >> >> >> >> Rühle
>>> >> >> >> >> >> >> napisał:
>>> >> >> >> >> >> >>>
>>> >> >> >> >> >> >>> Dear Steven,
>>> >> >> >> >> >> >>>
>>> >> >> >> >> >> >>> provided the same kBT was used, I can think of two
>>> >> >> >> >> >> >>> issues
>>> >> >> >> >> >> >>> which
>>> >> >> >> >> >> >>> might
>>> >> >> >> >> >> >>> lead to these differences
>>> >> >> >> >> >> >>>
>>> >> >> >> >> >> >>> 1) votca can shift the potential, but the shape should
>>> >> >> >> >> >> >>> match.
>>> >> >> >> >> >> >>> That
>>> >> >> >> >> >> >>> can
>>> >> >> >> >> >> >>> in
>>> >> >> >> >> >> >>> particular happen if you cut the rdf in a region where
>>> >> >> >> >> >> >>> there
>>> >> >> >> >> >> >>> are
>>> >> >> >> >> >> >>> still
>>> >> >> >> >> >> >>> modulations.
>>> >> >> >> >> >> >>> 2) What type of potential are you lookin at? For bonds
>>> >> >> >> >> >> >>> and
>>> >> >> >> >> >> >>> angles,
>>> >> >> >> >> >> >>> there
>>> >> >> >> >> >> >>> is indeed a normalization necessary, see
>>> >> >> >> >> >> >>>
>>> >> >> >> >> >> >>>
>>> >> >> >> >> >> >>>
>>> >> >> >> >> >> >>>
>>> >> >> >> >> >> >>>
>>> >> >> >> >> >> >>>
>>> >> >> >> >> >> >>>
>>> >> >> >> >> >> >>> http://dx.doi.org/10.1002/(SICI)1521-4044(199802)49:2/3<61::AID-APOL61>3.0.CO;2-V
>>> >> >> >> >> >> >>
>>> >> >> >> >> >> >>
>>> >> >> >> >> >> >> Thank you. I am looking at the nonbonded interactions
>>> >> >> >> >> >> >> only.
>>> >> >> >> >> >> >> The
>>> >> >> >> >> >> >> shape
>>> >> >> >> >> >> >> of
>>> >> >> >> >> >> >> the potential matches but the minima is lower than from
>>> >> >> >> >> >> >> my
>>> >> >> >> >> >> >> calulation.
>>> >> >> >> >> >> >> There
>>> >> >> >> >> >> >> is no normalization for non bonded so this is weird. I
>>> >> >> >> >> >> >> cut
>>> >> >> >> >> >> >> it
>>> >> >> >> >> >> >> at
>>> >> >> >> >> >> >> the
>>> >> >> >> >> >> >> begining as there were very small values and Votca was
>>> >> >> >> >> >> >> not
>>> >> >> >> >> >> >> able
>>> >> >> >> >> >> >> to
>>> >> >> >> >> >> >> extrapolate it properly.
>>> >> >> >> >> >> >>
>>> >> >> >> >> >> >>>
>>> >> >> >> >> >> >>>
>>> >> >> >> >> >> >>> Your second point indeed sounds a bit weired. Could
>>> >> >> >> >> >> >>> you
>>> >> >> >> >> >> >>> please
>>> >> >> >> >> >> >>> post
>>> >> >> >> >> >> >>> these
>>> >> >> >> >> >> >>> few curves to help debugging (i.e. the <name>.pot.cur,
>>> >> >> >> >> >> >>> <name>.pot.new
>>> >> >> >> >> >> >>> <name>.dist.tgt <name>.dist.new of the iteration 1
>>> >> >> >> >> >> >>> folder)?
>>> >> >> >> >> >> >>>
>>> >> >> >> >> >> >>> Victor
>>> >> >> >> >> >> >>
>>> >> >> >> >> >> >>
>>> >> >> >> >> >> >> Please, see attached.
>>> >> >> >> >> >> >>
>>> >> >> >> >> >> >>
>>> >> >> >> >> >> >>>
>>> >> >> >> >> >> >>>
>>> >> >> >> >> >> >>>
>>> >> >> >> >> >> >>> 2013/7/15 <[email protected]>
>>> >> >> >> >> >> >>>>
>>> >> >> >> >> >> >>>> Dear Votca Users,
>>> >> >> >> >> >> >>>>
>>> >> >> >> >> >> >>>> I have to issues with IBI:
>>> >> >> >> >> >> >>>>
>>> >> >> >> >> >> >>>> 1) I took one my ditributions and calculated on my
>>> >> >> >> >> >> >>>> own
>>> >> >> >> >> >> >>>> potential
>>> >> >> >> >> >> >>>> W=
>>> >> >> >> >> >> >>>> -kBT
>>> >> >> >> >> >> >>>> ln(RDF) and I got different potential than Votca
>>> >> >> >> >> >> >>>> provide
>>> >> >> >> >> >> >>>> me.
>>> >> >> >> >> >> >>>> For
>>> >> >> >> >> >> >>>> instance
>>> >> >> >> >> >> >>>> lets calculate the potential minimum for the
>>> >> >> >> >> >> >>>> distribution
>>> >> >> >> >> >> >>>> maximum
>>> >> >> >> >> >> >>>> of
>>> >> >> >> >> >> >>>> 162.
>>> >> >> >> >> >> >>>> Pot = -.249435*ln(164) = -12.69. The minimum of Votca
>>> >> >> >> >> >> >>>> potential
>>> >> >> >> >> >> >>>> corresponds
>>> >> >> >> >> >> >>>> to approximately -16 kJ/mol. Where I missed
>>> >> >> >> >> >> >>>> something? is
>>> >> >> >> >> >> >>>> it
>>> >> >> >> >> >> >>>> somehow
>>> >> >> >> >> >> >>>> normalized?
>>> >> >> >> >> >> >>>>
>>> >> >> >> >> >> >>>> 2) After 1st iteration my distribution was much
>>> >> >> >> >> >> >>>> higher
>>> >> >> >> >> >> >>>> than
>>> >> >> >> >> >> >>>> the
>>> >> >> >> >> >> >>>> target
>>> >> >> >> >> >> >>>> one so I guess the potential should decrease but
>>> >> >> >> >> >> >>>> apparently
>>> >> >> >> >> >> >>>> the
>>> >> >> >> >> >> >>>> new
>>> >> >> >> >> >> >>>> potential has deeper minima so the next distribution
>>> >> >> >> >> >> >>>> has
>>> >> >> >> >> >> >>>> a
>>> >> >> >> >> >> >>>> even
>>> >> >> >> >> >> >>>> higer
>>> >> >> >> >> >> >>>> distribution. Could anyone please explain me this?
>>> >> >> >> >> >> >>>>
>>> >> >> >> >> >> >>>> Steven
>>> >> >> >> >> >> >>>>
>>> >> >> >> >> >> >>>>
>>> >> >> >> >> >> >>>> --
>>> >> >> >> >> >> >>>> You received this message because you are subscribed
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>>> >> >> >> >> >> >>>> Groups "votca" group.
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>>> >> >> >> >> >> >>>> it,
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>>> >> >> >> >> >> >>>> To post to this group, send email to
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>>> >> >> >> >> >> >>>> Visit this group at
>>> >> >> >> >> >> >>>> http://groups.google.com/group/votca.
>>> >> >> >> >> >> >>>> For more options, visit
>>> >> >> >> >> >> >>>> https://groups.google.com/groups/opt_out.
>>> >> >> >> >> >> >>>>
>>> >> >> >> >> >> >>>>
>>> >> >> >> >> >> >>>
>>> >> >> >> >> >> >>>
>>> >> >> >> >> >> > --
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>>> >> >> >> >> >>
>>> >> >> >> >> >>
>>> >> >> >> >> >>
>>> >> >> >> >> >> --
>>> >> >> >> >> >> Christoph Junghans
>>> >> >> >> >> >> Web: http://www.compphys.de
>>> >> >> >> >> >
>>> >> >> >> >> > --
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>>> >> >> >> >> >
>>> >> >> >> >
>>> >> >> >> > --
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>>> >> >> >> >
>>> >> >> >>
>>> >> >> >>
>>> >> >> >>
>>> >> >> >> --
>>> >> >> >> Christoph Junghans
>>> >> >> >> Web: http://www.compphys.de
>>> >> >> >
>>> >> >> > --
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>>> >> >>
>>> >> >>
>>> >> >>
>>> >> >> --
>>> >> >> Christoph Junghans
>>> >> >> Web: http://www.compphys.de
>>> >> >
>>> >> > --
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>>> >> >
>>> >>
>>> >>
>>> >>
>>> >> --
>>> >> Christoph Junghans
>>> >> Web: http://www.compphys.de
>>> >
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>>>
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>>>
>>> --
>>> Christoph Junghans
>>> Web: http://www.compphys.de
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