Hey Christoph,

First of all thanks for following up. Sorry this is gonna be a long email
but I have some interesting observations based on your question. First of
all if I try to do a topology dump using my pdb file I don't get a "size"
of the box per say.

*I get this :- I have 8000 beads in 500 molecules*

*Boundary Condition: open*

Moreover the charges on my atoms are also 0 in the pdb file. I figured that
for my case, using the LAMMPS data file I generate from my PDB file is a
better candidate. When I use the LAMMPS data file, the identifiers change
from 1:ALA:XX to 1:DUM:XX. I did all those changes and then if I do
csg_dump I get:-





*I have 11000 beads in 500 moleculesBoundary Condition: orthorhombic Box
matix: 5.76509 0 0            0 5.76509 0            0 0 5.76509 (It was a
different data set which has 11000 molecules instead of 8000 so that is not
an issue)*

Now my original LAMMPS data file actually gies from:



*-28.825434 28.825434 xlo xhi-28.825434 28.825434 ylo yhi-28.825434
28.825434 zlo zhi*

So I guess VOTCA doesn't account for the negative coordinates and starts
everything from 0.

I tried csg_map with this and I again got the previous error:









*I have 11000 beads in 500 moleculesI have 500 beads in 500 molecules for
the coarsegraining-20.153  0.534-24.577  -18.68  3.621-23.916an error
occurred:coarse-grained bead is bigger than half the box (atoms N1 (id 18),
O7 (id 22) , molecule 1).*

This time around I just increased the box size by a multiplier of 10 in x,y
and z and it worked:












*These styles use the following formats in the atom block:atom-ID
molecule-ID atom-type charge x y zatom-ID molecule-ID atom-type charge x y
z nx ny nzatom-ID molecule-ID atom-type x y zatom-ID molecule-ID atom-type
x y z nx ny nzatom-ID atom-type x y zatom-ID atom-type x y z nx ny nzI have
11000 beads in 500 moleculesI have 500 beads in 500 molecules for the
coarsegrainingReading frame, timestep 5000000writing coarse-grained
trajectory to cg.gro*

I wanted to know whether increasing the box size is okay in this case.

Thanks again

On Wed, Jul 7, 2021 at 6:35 PM Christoph Junghans <[email protected]>
wrote:

> On Wed, Jul 7, 2021 at 2:18 PM Rishabh Guha <[email protected]> wrote:
> >
> > Hey Christoph,
> >
> > First of all in my pdb file the atoms are at:-
> > ATOM      1  CA  ALA A   1      11.853  -2.654   0.787  1.00  0.00
>      C
> > ATOM      2  C   ALA A   1      12.896  -2.967  -0.276  1.00  0.00
>      C
> >
> > Do you know why votca is dividing all the positions by 10?
> VOTCA uses nm internally, and pdb is in Angstrom.
> >
> > Secondly these atoms show up as-
> > 0 Name 1:ALA:CA Type CA Mass 12.0107 Resnr 0 Resname ALA Charge 0
> > 1 Name 1:ALA:C Type C Mass 12.0107 Resnr 0 Resname ALA Charge 0
> > when I do csg_dump --top topology.xml and are mapped as 1:ALA:CA 1:ALA:C
> 1:ALA:N 1:ALA:H1 1:ALA:H2 1:ALA:CB 1:ALA:HA 1:ALA:HB1 1:ALA:HB2 1:ALA:HB3
> 1:ALA:O 1:ALA:OXT 1:ALA:HXT in the alanine.xml file.
> >
> > I do not see any issues in the mapping to be honest
> When you run csg_dump, what does it tell you the box size is?
>
> Christoph
> >
> > Regards
> >
> > On Wed, Jul 7, 2021 at 4:09 PM Christoph Junghans <[email protected]>
> wrote:
> >>
> >>
> >>
> >> On Wed, Jul 7, 2021, 14:05 Rishabh Guha <[email protected]> wrote:
> >>>
> >>> Hey Christoph,
> >>>
> >>> Thanks for the pointers. I tried to look closely into my mapping files
> and compared them with the pdb files, but nothing jumped out at me.
> Although I admit that it can easily be an oversight-I have just started
> using Votca today. I have attached all my files below. Does anything jump
> out at you? I am convinced that none of my atoms are than half the box
> length away
> >>
> >> The error says there is an atom at 1.1853
> >> -0.2654 0.0787  and one at 1.2896 -0.2967 -0.0276 that you are trying
> to map in a cg bead. These are the ones you have to look at carefully in
> the mapping file.
> >>
> >> Christoph
> >>
> >>>
> >>>
> >>> Regards
> >>>
> >>> On Wed, Jul 7, 2021 at 3:55 PM Christoph Junghans <[email protected]>
> wrote:
> >>>>
> >>>>
> >>>>
> >>>> On Wed, Jul 7, 2021 at 13:44 Rishabh Guha <[email protected]> wrote:
> >>>>>
> >>>>> Hey Christoph,
> >>>>>
> >>>>> Thanks for the help. I got to know about the csg_dump command as
> soon as I sent you the email. For some reason, csg_dump is categorizing all
> the atoms as the valine molecules as ALA as well, which is counterintuitive
> to me.
> >>>>
> >>>> yeah, there is a way to fix this, these are just unique identifiers
> so it doesn’t matter a lot what they are as long as they are unique. This
> artifact mainly stems from the fact that the pdb format misses some
> molecule information.
> >>>>
> >>>>
> >>>>
> >>>>> But I did change my valine.xml file to:-
> >>>>>
> >>>>> <cg_molecule>
> >>>>>   <name>VAL</name>
> >>>>>   <ident>VAL</ident>
> >>>>>   <topology>
> >>>>>     <cg_beads>
> >>>>>       <cg_bead>
> >>>>>         <name>VAL</name>
> >>>>>         <type>VAL</type>
> >>>>>         <mapping>A</mapping>
> >>>>>         <beads>
> >>>>>            1:ALA:CA 1:ALA:C 1:ALA:N 1:ALA:H1 1:ALA:H2 1:ALA:CB
> 1:ALA:HA 1:ALA:CG1 1:ALA:HG11 1:ALA:HG12 1:ALA:HG13 1:ALA:CG2 1:ALA:HB
> 1:ALA:HG21 1:ALA:HG22 1:ALA:HG23 1:ALA:O 1:ALA:OXT 1:ALA:HXT
> >>>>>         </beads>
> >>>>>       </cg_bead>
> >>>>>   </cg_beads>
> >>>>>   </topology>
> >>>>>   <maps>
> >>>>>     <map>
> >>>>>       <name>A</name>
> >>>>>       <weights>12 12 14 1 1 12 1 12 1 1 1 12 1 1 1 1 16 16
> 1</weights>
> >>>>>     </map>
> >>>>>   </maps>
> >>>>> </cg_molecule>
> >>>>>
> >>>>> Now, I get a different error,
> >>>>> I have 8000 beads in 500 molecules
> >>>>> I have 500 beads in 500 molecules for the coarsegraining
> >>>>>  1.1853
> >>>>> -0.2654
> >>>>>  0.0787  1.2896
> >>>>> -0.2967
> >>>>> -0.0276
> >>>>> an error occurred:
> >>>>> coarse-grained bead is bigger than half the box
> >>>>>  (atoms CA (id 1), C (id 2) , molecule 1)
> >>>>>
> >>>>> Can you please tell me what I can change?
> >>>>
> >>>> this error means you are trying to map teo atoms that are more than
> half a box length away into one cg bead. The 6 number above are the
> positions.
> >>>>
> >>>> There can be multiple reasons for that error, most of the times it is
> a typo in the mapping file. If you are sure things are correct, you can
> always make the box bigger.
> >>>>
> >>>> Christoph
> >>>>>
> >>>>>
> >>>>>
> >>>>> On Wed, Jul 7, 2021 at 3:29 PM Christoph Junghans <
> [email protected]> wrote:
> >>>>>>
> >>>>>>
> >>>>>>
> >>>>>> On Wed, Jul 7, 2021 at 13:23 Rishabh Guha <[email protected]> wrote:
> >>>>>>>
> >>>>>>> Hello,
> >>>>>>>
> >>>>>>> I have an atomistic system consisting of 250 alanine and 250
> valine molecules. I am trying to use cg_map using the pdb file and the
> LAMMPS output trajectory, but I am getting an error:-
> >>>>>>> cannot find: <1:VAL:CA> in VAL
> >>>>>>> an error occurred:
> >>>>>>> mapping error: molecule 1:VAL:CA does not exist
> >>>>>>
> >>>>>> try to run “csg_dump --top topol.xml” and check how VOTCA labels
> things.
> >>>>>>
> >>>>>> Christoph
> >>>>>>
> >>>>>>>
> >>>>>>> My topology file looks like:-
> >>>>>>> <topology base="temp.pdb">
> >>>>>>> <molecules>
> >>>>>>> <clear/>
> >>>>>>> <define name="ALA" first="1" nbeads="13" nmols="250"/>
> >>>>>>> <define name="VAL" first="3251" nbeads="19" nmols="250"/>
> >>>>>>> </molecules>
> >>>>>>> </topology>
> >>>>>>>
> >>>>>>> My individual alanine and valine xml files are:-
> >>>>>>> Alanine-
> >>>>>>> <cg_molecule>
> >>>>>>>   <name>ALA</name>
> >>>>>>>   <ident>ALA</ident>
> >>>>>>>   <topology>
> >>>>>>>     <cg_beads>
> >>>>>>>       <cg_bead>
> >>>>>>>         <name>ALA</name>
> >>>>>>>         <type>ALA</type>
> >>>>>>>         <mapping>A</mapping>
> >>>>>>>         <beads>
> >>>>>>>            1:ALA:CA 1:ALA:C 1:ALA:N 1:ALA:H1 1:ALA:H2 1:ALA:CB
> 1:ALA:HA 1:ALA:HB1 1:ALA:HB2 1:ALA:HB3 1:ALA:O 1:ALA:OXT 1:ALA:HXT
> >>>>>>>         </beads>
> >>>>>>>       </cg_bead>
> >>>>>>>   </cg_beads>
> >>>>>>>   </topology>
> >>>>>>>   <maps>
> >>>>>>>     <map>
> >>>>>>>       <name>A</name>
> >>>>>>>       <weights>12 12 14 1 1 12 1 1 1 1 16 16 1</weights>
> >>>>>>>     </map>
> >>>>>>>   </maps>
> >>>>>>> </cg_molecule>
> >>>>>>>
> >>>>>>> Valine
> >>>>>>> <cg_molecule>
> >>>>>>>   <name>VAL</name>
> >>>>>>>   <ident>VAL</ident>
> >>>>>>>   <topology>
> >>>>>>>     <cg_beads>
> >>>>>>>       <cg_bead>
> >>>>>>>         <name>VAL</name>
> >>>>>>>         <type>VAL</type>
> >>>>>>>         <mapping>A</mapping>
> >>>>>>>         <beads>
> >>>>>>>            1:VAL:CA 1:VAL:C 1:VAL:N 1:VAL:H1 1:VAL:H2 1:VAL:CB
> 1:VAL:HA 1:VAL:CG1 1:VAL:HG11 1:VAL:HG12 1:VAL:HG13 1:VAL:CG2 1:VAL:HB
> 1:VAL:HG21 1:VAL:HG22 1:VAL:HG23 1:VAL:O 1:VAL:OXT 1:VAL:HXT
> >>>>>>>         </beads>
> >>>>>>>       </cg_bead>
> >>>>>>>   </cg_beads>
> >>>>>>>   </topology>
> >>>>>>>   <maps>
> >>>>>>>     <map>
> >>>>>>>       <name>A</name>
> >>>>>>>       <weights>12 12 14 1 1 12 1 12 1 1 1 12 1 1 1 1 16 16
> 1</weights>
> >>>>>>>     </map>
> >>>>>>>   </maps>
> >>>>>>> </cg_molecule>
> >>>>>>>
> >>>>>>> I have attached the pdb file for further reference.
> >>>>>>>
> >>>>>>> Can anyone please help me with this?
> >>>>>>>
> >>>>>>> --
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> .
> >>>>>>
> >>>>>> --
> >>>>>> Christoph Junghans
> >>>>>> Web: http://www.compphys.de
> >>>>>>
> >>>>>> --
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> >>>>>> ---
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> .
> >>>>>
> >>>>>
> >>>>>
> >>>>> --
> >>>>> Rishabh Debraj Guha(PhD student)
> >>>>>
> >>>>> Graduate Research Assistant
> >>>>> Mechanical and Aerospace Engineering
> >>>>> North Carolina State University
> >>>>> Contact:- +1-347-205-2280
> >>>>>
> >>>>> --
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> .
> >>>>
> >>>> --
> >>>> Christoph Junghans
> >>>> Web: http://www.compphys.de
> >>>>
> >>>> --
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> .
> >>>
> >>>
> >>>
> >>> --
> >>> Rishabh Debraj Guha(PhD student)
> >>>
> >>> Graduate Research Assistant
> >>> Mechanical and Aerospace Engineering
> >>> North Carolina State University
> >>> Contact:- +1-347-205-2280
> >>>
> >>> --
> >>> Join us on Slack: https://join.slack.com/t/votca/signup
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> .
> >>
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> .
> >
> >
> >
> > --
> > Rishabh Debraj Guha(PhD student)
> >
> > Graduate Research Assistant
> > Mechanical and Aerospace Engineering
> > North Carolina State University
> > Contact:- +1-347-205-2280
> >
> > --
> > Join us on Slack: https://join.slack.com/t/votca/signup
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> .
>
>
>
> --
> Christoph Junghans
> Web: http://www.compphys.de
>
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> .
>


-- 
Rishabh Debraj Guha(PhD student)

Graduate Research Assistant
Mechanical and Aerospace Engineering
North Carolina State University
Contact:- +1-347-205-2280

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