Yes, I had to take some time understanding GROMACS functionality to get it 
all working. 
Thank you for the advice!

On Friday, August 20, 2021 at 7:41:04 PM UTC-4 Christoph Junghans wrote:

>
>
> On Fri, Aug 20, 2021, 16:08 Satyen Dhamankar <[email protected]> wrote:
>
>> You are right, Christoph. I did end up catching that error... However, I 
>> am now facing an issue with energygroups. 
>>
>> PNM in energygrp-table is not an energy group
>>
>> Did you run make_index to create all these energy groups?
>
> Christoph
>
>>
>>
>> This is my mdp file:
>> ```
>> ; RUN CONTROL PARAMETERS
>> integrator               = sd
>> ; Start time and timestep in ps
>> tinit                    = 0 
>> dt                       = 0.004
>> nsteps                   = 1000000
>> ; For exact run continuation or redoing part of a run 
>> init_step                = 0 
>> ; mode for center of mass motion removal
>> comm-mode                = Linear
>> ; number of steps for center of mass motion removal
>> nstcomm                  = 1 
>> ; group(s) for center of mass motion removal
>> comm-grps                =   
>>
>> ; OUTPUT CONTROL OPTIONS
>> ; Output frequency for coords (x), velocities (v) and fo_ces (f) 
>> nstxout                  = 0 
>> nstvout                  = 0 
>> nstfout                  = 0 
>> ; Output frequency for energies to log file and energy file
>> nstlog                   = 0 
>> nstenergy                = 5000
>> ; Output frequency and precision for xtc file
>> nstxout-compressed       = 100 
>> compressed-x-precision   = 1000
>> ; Selection of energy groups
>> energygrps               = WT PNM_&_BB PNM_&_BA PNM_&_BI
>>
>> ; NEIGHBORSEARCHING PARAMETERS
>> ; nblist update frequency
>> nstlist                  = 5 
>> ; ns algorithm (simple or grid)
>> ns-type                  = Grid
>> ; Periodic boundary conditions: xyz, no, xy
>> pbc                      = xyz 
>> periodic_molecules       = no
>> ; nblist cut-off    
>> rlist                    = 1.4 
>>
>> ; OPTIONS FOR ELECTROSTATICS AND VDW 
>> ; Method for doing electrostatics
>> coulombtype              = User
>> rcoulomb-switch          = 0 
>> rcoulomb                 = 1.4 
>> ; Relative dielectric constant for the medium and the reaction field
>> epsilon_r                = 1 
>> epsilon_rf               = 80
>> ; Method for doing Van der Waals
>> vdw-type                 = User
>> ; cut-off lengths    
>> rvdw-switch              = 0 
>> rvdw                     = 1.4 
>> ; Apply long range dispersion corrections for Energy and Pressure
>> DispCorr                 = EnerPres
>> ; Extension of the potential lookup tables beyond the cut-off
>> table-extension          = 1 
>> ; Seperate tables between energy group pairs
>> Set energygrps           = PNM WT
>> energygrp_table          = PNM WT   WT WT
>> ; Spacing for the PME/PPPM FFT grid
>> fourierspacing           = 0.12
>> ; FFT grid size, when a value is 0 fourierspacing will be used
>> fourier_nx               = 0 
>> fourier_ny               = 0 
>> fourier_nz               = 0 
>> ; EWALD/PME/PPPM parameters
>> pme_order                = 4 
>> ewald_rtol               = 1e-05
>> ewald_geometry           = 3d
>> epsilon_surface          = 0 
>>
>> ; IMPLICIT SOLVENT ALGORITHM
>> implicit_solvent         = No
>>
>> ; GENERALIZED BORN ELECTROSTATICS
>> ; Algorithm for calculating Born radii
>> gb_algorithm             = Still
>> ; Frequency of calculating the Born radii inside rlist
>> nstgbradii               = 1
>> ; Cutoff for Born radii calculation; the contribution from atoms
>> ; between rlist and rgbradii is updated every nstlist steps
>> rgbradii                 = 2
>> ; Dielectric coefficient of the implicit solvent
>> gb_epsilon_solvent       = 80
>> ; Salt concentration in M for Generalized Born models
>> gb_saltconc              = 0
>> ; Scaling factors used in the OBC GB model. Default values are OBC(II)
>> gb_obc_alpha             = 1
>> gb_obc_beta              = 0.8
>> gb_obc_gamma             = 4.85
>> ; Surface tension (kJ/mol/nm^2) for the SA (nonpolar surface) part of GBSA
>> ; The default value (2.092) corresponds to 0.005 kcal/mol/Angstrom^2.
>> sa_surface_tension       = 2.092
>>
>> ; OPTIONS FOR WEAK COUPLING ALGORITHMS
>> ; Temperature coupling
>> Tcoupl                   = no
>> ; Groups to couple separately
>> tc-grps                  = System
>> ; Time constant (ps) and reference temperature (K)
>> tau_t                    = 0.2
>> ref_t                    = 330
>> ; Pressure coupling
>> Pcoupl                   = no
>> Pcoupltype               = isotropic
>> ; Time constant (ps), compressibility (1/bar) and reference P (bar)
>> tau_p                    = 0.5
>> compressibility          = 4.5e-5
>> ref_p                    = 1.0
>> ; Scaling of reference coordinates, No, All or COM
>> refcoord_scaling         = No
>>
>> ; GENERATE VELOCITIES FOR STARTUP RUN
>> gen-vel                  = no
>>
>> ; OPTIONS FOR BONDS
>> constraints              = none
>> cutoff-scheme = Group
>> ```
>> I was having trouble getting PNM as part of the energy group, so I added 
>> set energygrps to it, but it still doesnt seem to be working…
>> On Friday, August 20, 2021 at 5:33:05 PM UTC-4 Christoph Junghans wrote:
>>
>>> On Fri, Aug 20, 2021 at 3:26 PM Satyen Dhamankar <[email protected]> 
>>> wrote: 
>>> > 
>>> > I see that VOTCA is running the following command: 
>>> > gmx_196 grompp -n index.ndx -f grompp.mdp -p topol.top -o topol.tpr -c 
>>> conf.gro 
>>> > 
>>> > And I am getting the following error: 
>>> > Molecule type PNM contains no atoms 
>>> > 
>>> > This is my topol.top file: 
>>> > 
>>> > [ defaults ] 
>>> > 
>>> > ; nbfunc comb-rule gen-pairs fudgeLJ fudgeQQ 
>>> > 
>>> > 1 1 no 0.0 0.0 
>>> > 
>>> > [ atomtypes ] 
>>> > 
>>> > ; type mass charge ptype sigma epsilon 
>>> > 
>>> > TB 29.04 0.000 A 0.0000 1.0000 
>>> > 
>>> > TA 43.00 0.000 A 0.0000 1.0000 
>>> > 
>>> > TI 43.00 0.000 A 0.0000 1.0000 
>>> > 
>>> > TW 18.01 0.000 A 0.0000 1.0000 
>>> > 
>>> > [ moleculetypes ] 
>>> More a question for the gromacs mailing list, but I believe this 
>>> needs two [ moleculetype ] sections instead, see 
>>>
>>> https://github.com/votca/csg-tutorials/blob/master/urea-water/ibi/topol.top#L11-L23
>>>  
>>>
>>> Christoph 
>>> > 
>>> > ; Name nrexcel 
>>> > 
>>> > PNM 3 
>>> > 
>>> > WT 1 
>>> > 
>>> > [ atoms ] 
>>> > 
>>> > ; nr type resnr residue atom cgnr charge mass typeB chargeB massB 
>>> > 
>>> > 1 TB 1 PNM BB 1 0 29.04 
>>> > 
>>> > 2 TA 1 PNM BA 1 0 43.00 
>>> > 
>>> > 3 TI 1 PNM BI 1 0 43.00 
>>> > 
>>> > 4 TW 1 WT BW 1 0 18.01 
>>> > 
>>> > [ bonds ] 
>>> > 
>>> > ;ai aj funct c0 c1 c2 c3 
>>> > 
>>> > 1 2 8 1 1.0 
>>> > 
>>> > 2 3 8 1 1.0 
>>> > 
>>> > [ angles ] 
>>> > 
>>> > ;ai aj ak funct c0 c1 c2 
>>> > 
>>> > 1 2 3 8 1 1.0 
>>> > 
>>> > [ molecules ] 
>>> > 
>>> > ; 
>>> > 
>>> > PNM 1 
>>> > 
>>> > WT 4046 
>>> > 
>>> > I don’t understand what is causing this error. I have written similar 
>>> types of topol.top files, and it seems to be working just fine. 
>>> > 
>>> > For completeness sake, this is my conf.gro file: 
>>> > 
>>> > 
>>> > what a nice title 
>>> > 
>>> > 4049 
>>> > 
>>> > 1PNM BB 1 2.229 1.514 4.238 
>>> > 
>>> > 1PNM BA 2 2.272 1.286 4.235 
>>> > 
>>> > 1PNM BI 3 2.194 1.048 4.125 
>>> > 
>>> > 2WT BW 4 2.276 1.580 1.676 
>>> > 
>>> > 3WT BW 5 0.119 0.703 0.277 
>>> > 
>>> > 4WT BW 6 1.682 0.128 4.229 
>>> > 
>>> > 5WT BW 7 4.889 2.252 2.343 
>>> > 
>>> > . 
>>> > 
>>> > . 
>>> > 
>>> > . 
>>> > 
>>> > 4045WT BW 4047 1.332 2.041 1.148 
>>> > 
>>> > 4046WT BW 4048 0.125 1.348 4.089 
>>> > 
>>> > 4047WT BW 4049 0.839 1.673 3.898 
>>> > 
>>> > 4.98674 4.98674 4.98674 0.00000 0.00000 0.00000 0.00000 0.00000 
>>> 0.00000 
>>> > 
>>> > I would appreciate any advice you have for me! 
>>> > 
>>> > -- 
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>>>  
>>>
>>>
>>>
>>>
>>> -- 
>>> Christoph Junghans 
>>> Web: http://www.compphys.de 
>>>
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>> .
>>
>

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