you can if you put your js and css files in the view folder e.g.

controllers/default.py
def myscript():
  return dict()

def index():
  response.files.append(URL('myscript.js'))

views/default/myscript.js
jQuery(document).ready(function() {
  alert('this is an alert with a web2py url {{=URL('index')}}')
});

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