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Hi Zhanwu--

>  
>  Could I directly include J-coupling constant into the structure calculation
>  without convert to angle?  
>  

Yes. You do not need to convert J-couplings to torsion angle
restraints. Use the XPLOR couplings command to achieve this. For an
example, please see eginput/mef_dna/sa_mef_dipo.inp in the
xplor-nih-2.0.2 distribution. Note, however, that you do need to
provide the Karplus curve coefficients when using the couplings
command. 

Charles

-- 
Charles Schwieters     email: [email protected]
                       www:   http://schwieters.org/cds
phone: (301) 402-4914  FAX:   (301) 402-2867

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