Hi guys, I've been using XPLOR-NIH for a while now but until recently 
had not come across the following error:

--> ERROR: matrix is not a rotation matrix.

This seems to happen when I use a torsion angle dynamics protocol, but 
change my noe potential  from:

   potential  all soft

to:

   potential  all square


The protocol I'm using for simulated annealing works perfectly when I 
use the "soft" noe potential function, and dies with the aforementioned 
error if this is changed to "square".   I've tried to isolate the 
problem, but all I can conclude at this stage is that "soft" lets things 
work, and "square" doesn't.  Can anyone give me the low-down on what 
silly mistake I have probably made?  I did find something about ARIA/CNS 
giving this error when using a torsion angle dynamics protocol, and that 
it can be a timestep issue.  I feel quite happy with a 0.015 ps 
timestep  and find that reducing it to less desirable values fails to 
fix the problem.

Thanks in advance,

Haydyn

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Haydyn Mertens
PhD student
Department of Biochemistry
University of Melbourne
Australia

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