First, you shouldn?t use a single time point measurement but a two-time point measurement. The former is inaccurate. See: Iwahara, J., Tang, C. & Clore, G.M. (2007) Practical aspects of 1H transverse paramagnetic relaxation enhancement measurements on macromolecules. J. Magn. Reson. 184, 185-195.
Second, you should be refining directly against the PRE rates. See: Iwahara, J., Schwieters, C. D., Clore, G. M. (2004) Ensemble approach for NMR structure refinement against 1H paramagnetic relaxation enhancement data arising from a flexible paramagnetic group attached to a macromolecule. J. Am. Chem. Soc. 126, 5879-5896. On 4/7/08 10:37 AM, "Suja Naren" <sujanaren at yahoo.com> wrote: > > Hi, > I like to estimate the distance from PRE single time point measurement. > Can anyone help me with this? > > Thanks a lot, > Suja > > > > You rock. That's why Blockbuster's offering you one month of Blockbuster Total > Access > <http://us.rd.yahoo.com/evt=47523/*http://tc.deals.yahoo.com/tc/blockbuster/te > xt5.com> , No Cost. > > _______________________________________________ > Xplor-nih mailing list > Xplor-nih at nmr.cit.nih.gov > http://dcb.cit.nih.gov/mailman/listinfo/xplor-nih -- G. Marius Clore, MD, PhD Chief, Protein NMR Section Laboratory of Chemical Physics Bldg 5/Rm B1-30I NIDDK, National Institutes of Health Bethesda, MD 20892-0520 Tel: (301) 496 0782 Fax: (301) 496 0825 E-mail: mariusc at intra.niddk.nih.gov web: http://spin.niddk.nih.gov/clore -------------- next part -------------- An HTML attachment was scrubbed... URL: http://dcb.cit.nih.gov/pipermail/xplor-nih/attachments/20080407/a4e43dcd/attachment.html
