Hi everyone, I have generated a peptide(22 residues) 20 acceptable structures only based on a set of NOE restraints using ??tutorial/nmr_torsion/torsion.inp??. the result showed the peptide contained 2 helix ranges and had good RMSDs, but ramachandran plot is not satisfying. Then, as John Kuszewski??s suggestions. I used eginput/gb1_rdc/anneal.py script to generate structures in order to use rama potential and I replaced the rdc restrains with kong.tbl. but I got a coil structure and many dihedral angles is in the range of ?? sheet in ramachandran plot. Which is dramaticly different from the result which were generate by torsion.inp. Could anyone help me? thanks
guohua xu
