Hello, I have some NMR structures obtained via CS-Rosetta. I also have SAXS structures of them. I would like to incorporate SAXS constrains into the structure calculations based on chemical shifts and I can't find out how to do that on Xplor.
I have collected NOE as well as triple resonance data. But I can't assign the protein completely because of the nature of the protein (4-helix bundle) and therefore, my NOE data is pretty much useless (so as PRE). I can fit the structures from CS-Rosetta into the SAXS structures. But I would like to actually use SAXS constrains to get the structures with NMR. Would you please assist me with this? Thank you very much! Faraz Harsini ________________________________ From: Charles Schwieters <[email protected]> Sent: Tuesday, April 9, 2019 11:22 PM To: Harsini, Faraz Subject: Xplor-NIH mailing list posting CAUTION: This email originated from outside of TTUHSC. Do not click links or open attachments unless you recognize the sender and know the content is safe. Please repost your message to the Xplor-NIH mailing list. I've included it below. I have added you to the list, so that your post should be accepted this time. Unfortunately, many aspects of the mailing list are currently broken. When I return from my travels my first order of business is to fix this list. thank you-- Charles I have some NMR structures obtained via CS-Rosetta. I also have SAXS structures of them. I would like to incorporate SAXS constrains into the structure calculations based on chemical shifts and I can't find out how to do that on Xplor. I have collected NOE as well as triple resonance data. But I can't assign the protein completely because of the nature of the protein (4-helix bundle) and therefore, my NOE data is pretty much useless. I can fit the structures from CS-Rosetta into the SAXS structures. But I would like to actually use SAXS constrains to get the structures with NMR. Would you please assist me with this? Thank you very much! Faraz Harsini PS. I tried to subscribe and it says forbidden I don't know why! Faraz Harsini, Ph.D. Department of Cell Physiology and Molecular Biophysics Center for Membrane Protein Research School of Medicine Texas Tech University Health Sciences Center [email protected] 806-543-4468
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