Hi Charlie,
yes you are right, but I assumed if people see a cloud of condensed
fog over their LN2 bath they should remove that by
a) filling up the bowl completely e.g. some LN2 drips out of the bowl
b) blow the fog away before you dip
True; this has been demonstrated quite rigorously:
A general method for hyperquenching protein crystals
Journal Journal of Structural and Functional Genomics
Volume 8, Number 4 / December, 2007
DOI 10.1007/s10969-007-9029-0
Pages 141-144
AND
J. Appl. Cryst. (2006). 39, 805-811 [ doi:10.1107/S0021889806037484 ]
Hyperquenching for protein cryocrystallography
M. Warkentin, V. Berejnov, N. S. Husseini and R. E. Thorne
------------------------------------------------------------------------
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Patrick J. Loll, Ph. D.
Professor of Biochemistry & Molecular Biology
Director, Biochemistry Graduate Program
Drexel University College of Medicine
Room 10-102 New College Building
245 N. 15th St., Mailstop 497
Philadelphia, PA 19102-1192 USA
(215) 762-7706
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