On 26 Sep 2010, at 9:00 AM, Seema Nath wrote: > I'm working with a protein which crystallizes in a mixture of PEG6K with 0.2M > AmSO4,my question is if there's any problem if I want to soak heavy metal > derivatives in this crystallizing condition? Does AmSO4 interfere in > heavy-metal soaking ? if yes, what's the reason? > Thank you in advance. > There's a nice discussion of heavy atom chemistry and why ammonium ions can be problematic in Greg Petsko's review from the 80's:
Methods Enzymol. 1985;114:147-56 The bottom line is that amines (and ammonium is an amine) can participate in coordination chemistry for certain metals. If memory serves some of this is also covered in Blundell and Johnson (? not sure about that, I don't have my copy at hand). Good luck, Pat --------------------------------------------------------------------------------------- Patrick J. Loll, Ph. D. Professor of Biochemistry & Molecular Biology Director, Biochemistry Graduate Program Drexel University College of Medicine Room 10-102 New College Building 245 N. 15th St., Mailstop 497 Philadelphia, PA 19102-1192 USA (215) 762-7706 pat.l...@drexelmed.edu
