Dear Phil,

I remember one case where reading a sequence file in PIR format failed, although I did my best to have the right format. It turned out, that the error message about the "wrong PIR format" was misleading - the real cause was a non-standard amino acid letter, in this case a "X". Maybe, you could check your sequence for this ...

Best regards,

Dirk.


Am 12.08.11 11:16, schrieb Phil Evans:
I was missing the semicolon, but it still fails

On 12 Aug 2011, at 10:12, Antony Oliver wrote:

Interesting iPhone formatting things going on...  Let's try again...

First line is "greater than symbol">  followed by some text about your protein 
then closed with a semicolon. The next line is blank. The next line contains your amino acid 
sequence, which you can also close with an optional asterisk.

Sent from my iPhone

On 12 Aug 2011, at 10:06, "Antony Oliver"<antony.oli...@sussex.ac.uk>  wrote:

PIR is fairly similar to Fasta, from addled memory the format is...

protein name;
----empty line----
MPREIL...rest of amino acid sequence with an optional asterisk to mark the 
sequence end.

Tony

Sent from my iPhone

On 12 Aug 2011, at 09:14, "Phil Evans"<p...@mrc-lmb.cam.ac.uk>  wrote:

Can anyone get this server to work? For me it keeps complaining that my 
sequence file is not a PIR file. The file looks OK to me, but I've never really 
understood what a PIR file is

Phil

On 12 Aug 2011, at 01:39, Kevin Jin wrote:

Should we really have some crystallographers to review and qc those structures 
before the formal releasing?  JCSG has set a very good mechanism for this issue.

There is a sever for self check.

http://smb.slac.stanford.edu/jcsg/QC/






On Thu, Aug 11, 2011 at 4:58 PM, Jacob Keller<j-kell...@fsm.northwestern.edu>  
wrote:
I think they fudged the data in this paper...

JPK

On Thu, Aug 11, 2011 at 6:30 PM, David Schuller<dj...@cornell.edu>  wrote:
link: http://iai.asm.org/cgi/reprint/IAI.05661-11v1

Ferric C. Fang&  Arturo Casadevall
Retracted Science and the Retraction Index
Infec. Immun. doi:10.1128/IAI.05661-11

Abstract: Articles may be retracted when their findings are no longer
considered trustworthy due to scientific misconduct or error, they
plagiarize previously published work, or are found to violate ethical
guidelines. Using a novel measure that we call the “retraction index,” we
found that the frequency of retraction varies among journals and shows a
strong correlation with the journal impact factor.
...

(with special attention to Figure 1, Retraction Index vs. Impact Factor)


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                            David J. Schuller
                            modern man in a post-modern world
                            MacCHESS, Cornell University
                            schul...@cornell.edu



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