Certainly not unprecedented, or even that unusual (I remember making gels from BSA and IgG solutions during grad school rotations). Gel formation usually requires crosslinking, so consider whether you might be getting adventitious disulfide bond formation. Pat
On 30 Aug 2011, at 11:31 AM, aidong wrote: > Dear Buddies, > > Sorry for bothering you with an off-ccp4 question. We recently are > experiencing a very strange phenomena. A couple of protein preps with > reasonably high concentration (10-20mg/ml) become a jelly after storages for > overnight or a couple of days at 4C. All of them have been purified by gel > filtration. Some of these proteins behave like this from very first preps > but some of them had been very kind to us previously. We have googled > extensively in CCP4BB and www but it appears this only happens to us. It > would be highly appreciated that you could exchange their experiences or > provide your suggestions. > > Aidong Han, Ph.D > > Department of Biomedical Sciences > School of Life Sciences > Xiamen University > Xiamen, Fujian 361005 > China > Phone: 0592-218-8172 (O) > 0592-218-8173 (L) > Web: http://life.xmu.edu.cn/adhanlab/
