Hi Florian - I've used Geneious for a few years now and been pleased with it. Also a "freemium" business model: Basic version is free, and Pro version price depends on the term and type of license (student, academic/government, or commercial). I find the Basic version suits my limited molecular biology needs pretty well. They also have occasional "Geneious Days" (today happens to be one!) when the Basic version can use all the features of the Pro version.
Available for Linux/Mac/Windows in both 32- and 64-bit. http://www.geneious.com/ -- Jared Sampson Xiangpeng Kong Lab NYU Langone Medical Center 550 First Ave MSB 329 New York, NY 10016 212-263-7898 On Sep 27, 2011, at 2:32 PM, Luca Jovine wrote: Hi Florian, Have a look at Serial Cloner - it's free, runs on OS X, Linux and Windows, and is really quite powerful - including the ability to export single or multiple sequences to FASTA format text files (however, it can only align two sequences at the time I'm afraid): http://serialbasics.free.fr/Serial_Cloner.html HTH, Luca ---------------------------------------------------------------- Luca Jovine, Ph.D. Assistant Professor & EMBO Young Investigator Karolinska Institutet Department of Biosciences and Nutrition & Center for Biosciences Hälsovägen 7, SE-141 83 Huddinge, Sweden Voice: +46.(0)8.524-81136 FAX: +46.(0)8.6081-501 E-mail: luca.jov...@ki.se<mailto:luca.jov...@ki.se> W3: http://jovinelab.org<http://jovinelab.org/> ---------------------------------------------------------------- On 27 Sep 2011, at 17:42 , Florian Schmitzberger wrote: Dear All, What type of software are people commonly using these days for vector/plasmid map editing, making/visualizing vector maps, and aligning (small to medium size) DNA sequencing data? Preferably, it should not be too expensive and be able to write text files, readable by other programs. I am familiar with VectorNTI, which is great for vector visualization and editing; but I find it somewhat expensive. Sequencher seems good to quickly align DNA sequences (such as from DNA sequencing) with templates, but is not free. I have been using ApE for while for alignments, but aligning many sequences is more cumbersome than in Sequencher; I have not tested if Sequencher is good at visualizing and editing plasmid maps. Ideally, I would like to have a single program for both purposes (vector editing and DNA sequence comparison). Does something like that exist? What are the alternatives to above programs? Thank you in advance. Florian ----------------------------------------------------------- Florian Schmitzberger, PhD Biological Chemistry and Molecular Pharmacology Harvard Medical School 250 Longwood Avenue, Seeley G. Mudd 123 Boston, MA 02115, US Tel: 001 617 432 5603 </PRE> <html> <body> ------------------------------------------------------------<br /> This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email.<br /> ================================= </body> </html> <PRE>
