On Dec 20, 2012, at 8:16 PM, dengzq1987 <dengzq1...@gmail.com> wrote:
> Hi all, > recently, i perform an EMSA experiment.when i prepared the DNA with a 5' > poly(G)-tailed ,i find that it formed guanine tetraplexes .how can i prevent > this happen,or has any method to disrupt the guanine tetraplexes structure? > any suggestion is appreciation. > > > dengzq Unless it has some other, more stable structure to compete it out, you will have this problem persist. Even GTP in solution will do this. Bill William G. Scott Professor Department of Chemistry and Biochemistry and The Center for the Molecular Biology of RNA 228 Sinsheimer Laboratories University of California at Santa Cruz Santa Cruz, California 95064 USA
