Hi,
I observed a very similar hexagonal arrangement of electron density blobs
in one of my structures recentely and asked the CCP4bb community to help
me explain it. Unfortunately, noone could come up with some satisfactory
explanation, so I gave up on interpreting this rogue density. What was
certain beyond doubt is that it was somehow caused by the presence of
cobalt ions. Do you, by chance, also have cobalt or similar ions present
in your crystallization condition?
Regards,
Joern
******************************************
Address:
Joern Krausze
Molecular Structural Biology
Helmholtz Centre for Infection Research
Inhoffenstrasse 7
38124 Braunschweig
Germany
Email: joern.krau...@helmholtz-hzi.de
Phone: +49 (0)531 6181 7023 (office)
+49 (0)531 6181 7020 (lab)
******************************************
On Mon, 4 Feb 2013, Gang Dong wrote:
Dear all,
Here are some “hexmeric” densities we observed in our 1.6-A resolution 2Fo-Fc
map. They are located in between two dimers. Although 7 waters would fit nicely
in the densities,
we are not sure whether they might be something else (metals?). Any suggestions
are welcome. Thanks! Gang
[IMAGE]
__________________________________
Gang Dong, PhD
Junior Group Leader
Max F. Perutz Laboratories (MFPL)
Dr. Bohrgasse 9/3
A-1030 Vienna, Austria
Phone: +43-1-4277-61625
FAX: +43-1-4277-9616
http://www.mfpl.ac.at/mfpl-group/group/dong.html
________________________________
Helmholtz-Zentrum für Infektionsforschung GmbH | Inhoffenstraße 7 | 38124
Braunschweig | www.helmholtz-hzi.de
Vorsitzende des Aufsichtsrates: MinDir’in Bärbel Brumme-Bothe,
Bundesministerium für Bildung und Forschung
Stellvertreter: Rüdiger Eichel, Abteilungsleiter Niedersächsisches Ministerium
für Wissenschaft und Kultur
Geschäftsführung: Prof. Dr. Dirk Heinz; Ulf Richter, MBA
Gesellschaft mit beschränkter Haftung (GmbH)
Sitz der Gesellschaft: Braunschweig
Handelsregister: Amtsgericht Braunschweig, HRB 477
