Re: [ccp4bb] cryo condition

[Boaz Shaanan]

Hi, Paraton oil worked nicely for me for these conditions.           Boaz    Boaz Shaanan, Ph.D.                                         Dept. of Life Sciences                                      Ben-Gurion University of the Negev                          Beer-Sheva 84105                                            Israel                                                                                                                  E-mail: bshaa...@bgu.ac.il Phone: 972-8-647-2220  Skype: boaz.shaanan                  Fax:   972-8-647-2992 or 972-8-646-1710                          From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Faisal Tarique [faisaltari...@gmail.com] Sent: Thursday, May 23, 2013 12:42 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] cryo condition Dear all Can anybody tell me the appropriate cryo condition for the crystals obtained in 2M Ammonium sulphate and tris pH8.5..I tried to add 10% glycerol to it but still the ice ring is forming.. Thanx in advance -- Regards Faisal School of Life Sciences JNU