Hi ccp4 experts, I have collected diffraction images to 0.96 Angstrom resolution to the edge of the detector. One data set give me the full completeness and below i have pasted my statistic values got from XDS.
I have cut off data at 1.12 A which seems to be quite nice regarding all necessary parameter to consider. But the problem is the Rfree is 0.176 and Rwork is 0.151 but the maps look very good. Even If I cut off the data to 1.15 Angstrom the R factors not improved. The space group of my complex is P21212. I used anisotropic Bfactors, add Hydrogen on and also used TLS but unfortunately i can not reduce able to reduce R factor in a good way. so, could you people kindly give me some suggestion how can i improve my data quality. RESOLUTION NUMBER OF REFLECTIONS COMPLETENESS R-FACTOR R-FACTOR COMPARED I/SIGMA R-meas CC(1/2) Anomal SigAno Nano LIMIT OBSERVED UNIQUE POSSIBLE OF DATA observed expected Corr 1.12 104562 21143 21268 99.4% 37.0% 37.8% 103982 4.04 41.4% 91.8* 0 0.767 15016 1.04 86588 21777 23100 94.3% 98.4% 95.8% 84962 1.44 113.0% 74.1* -1 0.700 10431 I would be thankful for your valuable comments AFSHAN
