Perhaps you should try finding buffer conditions and protein concentration that pushes the self-association equilibrium to one particular oligomeric state.
Sent from Jack's iPad On Sep 12, 2013, at 9:53 AM, "Debasish Kumar Ghosh" <dkgh...@cdfd.org.in> wrote: > Hi, > > I am working with a protein which can assume different oligomerization forms, > starting from monomers to trimers and even penta-decamers. We conformed this > by Native PAGE and HPLC studies. The protein's theoretical monomeric > molecular weight is 14.6 KDa (pI - 5.9) and it has some 140 amino acids with > high Glutamic acid (24), Lysine (10) and Arginine (13) content. I have tried > to crystallize it but not getting any hit as far now. > Previous study showed that this protein gets some stability by Calcium ion. > With the calcium chloride conditions, I am getting spherical shaped > structures, but not sure what are they; calcium chloride crystals or protein > crystals. Can protein crystals be spherical in shape, specially when the > protein behaves like an oligomer? > Also please let me know what is the minimum protein concentration required to > obtain crystal for such small protein (if there is any empirical rule/idea). > Any suggestion will be highly appreciated. > > Thanks and regards, > Debasish Kumar Ghosh > > CSIR- Junior Research Fellow (PhD Scholar) > C/o: Dr. Akash Ranjan > Computational and Functional Genomics Group > Centre for DNA Fingerprinting and Diagnostics > Hyderabad, INDIA > > Email(s): dkgh...@cdfd.org.in, dgho...@gmail.com > Telephone: 0091-9088787619 (M), 0091-40-24749396 (Lab) > Lab URL: > http://www.cdfd.org.in/labpages/computational_functional_genomics.html
