Dear crystallographers,
I have solved a structure of a glucose
binding protein of CE4 family. When I try to solve the structure using the
same CE4 family enzyme as search model, it failed for many case. Finally, I
solved the with a same family enzyme used as search model. As soon as I
solved the structure, I superposed my final refined model with structures
of CE4 family enzymes which did not produce the good molecular replacement
solution for my enzyme. I found that all are having (Beta/alpha)7 fold and
superpose very well with my model. Whereas, some loop region are not
superpose very well. My doubt is why molecular replacement failed thought
over-all fold is same?.
--
*Dhanasekaran Varudharasu*
Post-Doctoral Fellow
Department of Oral Biology
Rutgers school of Dental Medicine
Rutgers Biomedical and Health Sciences
Newark, NJ 07103
USA
