But I think he was asking about imaging of intrinsic fluorescence of protein crystals…
I like your idea about gel imaging, though. JPK From: Hughes, Jon [mailto:jon.hug...@bot3.bio.uni-giessen.de] Sent: Wednesday, April 05, 2017 8:23 AM To: Keller, Jacob <kell...@janelia.hhmi.org>; CCP4BB@JISCMAIL.AC.UK Subject: AW: [ccp4bb] UVEX UV Fluorescence for ethidium bromide you need a 590nm (50-100nm FWHH) bandpass interference filter. ccd chips are sensitive to infrared and transilluminators produce a lot of it! best j Von: Keller, Jacob [mailto:kell...@janelia.hhmi.org] Gesendet: Mittwoch, 5. April 2017 14:16 An: Hughes, Jon <jon.hug...@bot3.bio.uni-giessen.de<mailto:jon.hug...@bot3.bio.uni-giessen.de>>; CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK> Betreff: RE: [ccp4bb] UVEX UV Fluorescence Why 590 nm? BP, SP, LP? I would have thought 390 nm LP or similar--was it a typo? JPK From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Hughes, Jon Sent: Wednesday, April 05, 2017 6:43 AM To: CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK> Subject: [ccp4bb] AW: [ccp4bb] UVEX UV Fluorescence you can save a fortune by just fitting a 590nm interference filter to the front of a ccd camera attached to a PC + a UV/B transilluminator. i set this up in my lab for about €1000 total 15 years ago and we've used it everyday since. cheers jon Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von Cyprian Cukier Gesendet: Mittwoch, 5. April 2017 12:26 An: CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK> Betreff: [ccp4bb] UVEX UV Fluorescence Dear All, We are about to buy a new imaging system for our laboratories and we consider UVEX UV Fluorescence Imaging from MD. Can anyone provide some comments about this equipment (eg. is it reliable, robust, no technical issues, low-maintenance etc.)? All comments will be appreciated. Thanks, Cyprian
