Dear community,
the autonomous data collection beamline MASSIF-1 at the ESRF has now
been running for 3 years and is highly successful. The service is
available for all types of sample mount and crystals, from screening
initial hits from crystallisation screens through to the collection of
hundreds of data sets from well characterised systems.
Recent developments:
* We now pay for dewar shipping to and from the ESRF for all peer
reviewed academic experiments on MASSIF-1 (1 dewar per shift or 3
dewars for 24 hours). See http://www.esrf.eu/dewar for details.
* Beam size is now adapted dynamically to the best diffraction volume
in each crystal.
* Multi position data collection and helical data collection now
available.
* You can now apply for completely automated high throughput fragment
screening and data collection (in collaboration with the HTX lab at
EMBL Grenoble) via iNEXT, see http://www.inext-eu.org/access/
Essential points:
1. Time is not scheduled but booked on-line when you need it via an
online calendar in consultation with your BAG responsible - you can book
regular slots or at short notice when you get crystals
2. If you are not part of a BAG you are welcome to apply for rapid
access time here http://www.esrf.eu/MXnon-BAGproposal
3. No restriction on sample type - a rapid X-ray centring routine scans
the full sample mount to locate and centre crystals (crystals <20um are
regularly seen) If there is a crystal there MASSIF will find it!
4. Data collections can be tailored to each sample (resolution, space
group, beam size, redundancy, SAD and more) via the ISPyB diffraction
plan (http://www.esrf.fr/MXPressE)
5. Full results available in real time via ISPyB
6. No need to come to the beamline
7. Time deducted as a function of the number of samples processed
MASSIF-1 has a beam of 100 x 65 um (HxV), that can be defined by
apertures from 50 um down to 10 um, and has a fixed energy of 12.8 keV
with a flux of ~3 x 10e12 ph/s and a PILATUS3 2M detector.
The service has now been running for 3 years and is proving very
popular, we have now processed over 30,000 crystals and many structures
have been solved de novo by SAD. Details of the process and the beamline
can be found in some recent publications:
Svensson, O., Monaco, S., Popov, A. N., Nurizzo, D. & Bowler, M. W.
(2015). The fully automatic characterization and data collection from
crystals of biological macromolecules, Acta Cryst. D71, 1757-1767,
http://dx.doi.org/10.1107/S1399004715011918
Bowler, M.W., Nurizzo, D., et al. (2015). MASSIF-1: A beamline dedicated
to the fully automatic characterisation and data collection from
crystals of biological macromolecules, J. Synchrotron Rad. 22,
1540-1547, http://dx.doi.org/10.1107/S1600577515016604.
Bowler, M.W., Svensson, O. & Nurizzo, D. (2016): Fully automatic
macromolecular crystallography: the impact of MASSIF-1 on the optimum
acquisition and quality of data, Crystallography Reviews,
http://dx.doi.org/10.1080/0889311X.2016.1155050.
Nurizzo, D., Bowler M.W., et al. (2016) RoboDiff: combining a sample
changer and goniometer for highly automated macromolecular
crystallography experiments Acta Cryst D72, 966-975,
http://dx.doi.org/10.1107/S205979831601158X
For any further questions please don't hesitate to contact me
(mbow...@embl.fr) or Didier Nurizzo (nuri...@esrf.fr).
Best wishes, Matthew.
Web:http://www.esrf.eu/MASSIF1
Mail: mass...@esrf.fr
FAQ:http://www.esrf.eu/MASSIF_FAQ
--
Matthew Bowler
Synchrotron Crystallography Group
European Molecular Biology Laboratory
71 avenue des Martyrs
CS 90181 F-38042 Grenoble
France
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Tel: +33 (0) 4.76.20.76.37
Fax: +33 (0) 4.76.20.71.99
http://www.embl.fr/
http://www.esrf.eu/MASSIF1
https://twitter.com/id30_massif1
http://www.embl.fr/research/unit/mccarthy/members/index.php?s_personId=CP-60015559
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