Have to do primer walking in a cdna library first. Artem
On Jul 24, 2017 7:26 AM, "syed ibrahim" < 0000048c02cac012-dmarc-requ...@jiscmail.ac.uk> wrote: Hello All I am interested in cloning a gene from a plant. I searched the database only partial sequence is available, ie: for 160 residues only. The full length of the protein is around 570 residues. I designed forward primer and I have no clue to design reverse primer. Any help Thank you Syed