Hi, This might look as a very fundamental question. I have a dataset of crystal structures better than 3.5Ang resolution. For a qualitative analysis, I want to compare the residue-wise B-factors in these structures, but due to different procedures adopted in refinement and scaling, I understand that these values cannot be compared in a raw manner.
Can someone suggest appropriate normalization methods that could be used for scaling these B-factors for a relevant and meaningful comparison? All the files have isotropic B-factor values and there are no ANISOU entries in any of the files. Thanks Asmita