Dear Manoj, I'm happy to answer your questions and have a look at your data. Maybe we can take this off-list and you report back if and when you solve your structure.
By the way, I'm processing native data collected with a MetalJet at the moment. The anomalous signal is even weaker at 1.35 Å but can be useful. All best. Andreas On Tue, Apr 3, 2018 at 5:36 PM, Manoj Saxena <manoj.sax...@upr.edu> wrote: > Thank you!, Andreas and all others who replied. > I have seen your tutorial and that's what I was referring to. > Maybe I can consult you later when I screen some samples and have an idea > of signal strength? > > Regards > Manoj > > > 2018-04-03 10:55 GMT-04:00 Andreas Förster <andreas.foers...@dectris.com>: > >> Dear Manoj, >> >> providing your crystals diffract to at least 2.5 Å and the sulfurs are >> ordered, you should be able to solve your structure without too many >> problems. As a first experiment, I would recommend collecting 360 degrees >> of data. Process these with XDS and the FRIEDEL'S_LAW= FALSE option and >> see how much anomalous signal CORRECT.LP reports, if you've got stars in >> the values of the Anomal Corr column, you're on the right track. Try to >> phase with SHELXD, following the advice on http://shelx.uni-goettingen >> .de/tutorials.php. >> >> If you don't succeed, you might need higher multiplicity. If you have a >> kappa or chi goniometer, you can reorient the crystal and collect another >> 360 degrees and another 360 degrees, and so on. Merge all data in XSCALE >> until you can solve the structure. >> >> I once wrote a little tutorial for doing S-SAD on the home source that >> you might want to follow. http://www.imperial.ac.uk/x-ra >> y-crystallography/learning-more/sulphur-sad/ With an HPC detector (or >> even an image plate) you'd getter better data than with a CCD detector >> and be able to solve your structure quicker. (Disclaimer: I work for a >> company that makes HPC detectors.) I consider the home source a great >> place to solve structures by native SAD. >> >> All best. >> >> >> Andreas >> >> >> >> On Tue, Apr 3, 2018 at 4:26 PM, Manoj Saxena < >> 00001d16aa30e8a1-dmarc-requ...@jiscmail.ac.uk> wrote: >> >>> Hi All, >>> >>> I am writing to seek advice on doing sulphur SAD data collection >>> at Cu based home source for a protein that is 12 KDa and has 6 S atoms. >>> I have seen some links online and some references but would be grateful >>> if >>> you can share your know-how for success with this. >>> Like what multiplicity of data would be good to aim for and >>> data processing tips. >>> Inputs from people who have tried and failed would also be highly >>> appreciated. >>> >>> Thank you >>> Manoj Saxena >>> University of Puerto Rico >>> >>> >>> >> >> >> -- >> <https://www.dectris.com> >> Andreas Förster, Ph.D. >> MX Application Scientist, Scientific Sales >> Phone: +41 56 500 21 00 <+41%2056%20500%2021%2000> | Direct: +41 56 500 >> 21 76 <+41%2056%20500%2021%2076> | Email: andreas.foers...@dectris.com >> DECTRIS Ltd. | Taefernweg 1 >> <https://maps.google.com/?q=Taefernweg+1+%C2%A0+%7C+5405+Baden-Daettwil+%C2%A0+%7C%C2%A0Switz+erland&entry=gmail&source=g> >> >> <https://maps.google.com/?q=Taefernweg+1+%C2%A0+%7C+5405+Baden-Daettwil+%C2%A0+%7C%C2%A0Switz+erland&entry=gmail&source=g>| >> 5405 Baden-Daettwil >> <https://maps.google.com/?q=Taefernweg+1+%C2%A0+%7C+5405+Baden-Daettwil+%C2%A0+%7C%C2%A0Switz+erland&entry=gmail&source=g> >> >> <https://maps.google.com/?q=Taefernweg+1+%C2%A0+%7C+5405+Baden-Daettwil+%C2%A0+%7C%C2%A0Switz+erland&entry=gmail&source=g> >> | Switz >> <https://maps.google.com/?q=Taefernweg+1+%C2%A0+%7C+5405+Baden-Daettwil+%C2%A0+%7C%C2%A0Switz+erland&entry=gmail&source=g> >> erland >> <https://maps.google.com/?q=Taefernweg+1+%C2%A0+%7C+5405+Baden-Daettwil+%C2%A0+%7C%C2%A0Switz+erland&entry=gmail&source=g> >> >> <https://maps.google.com/?q=Taefernweg+1+%C2%A0+%7C+5405+Baden-Daettwil+%C2%A0+%7C%C2%A0Switz&entry=gmail&source=g> >> | >> <https://maps.google.com/?q=Taefernweg+1+%C2%A0+%7C+5405+Baden-Daettwil+%C2%A0+%7C%C2%A0Switz&entry=gmail&source=g> >> www.dectris.com >> >> [image: LinkedIn] <https://www.linkedin.com/company/5067919> >> [image: facebook] >> <https://www.facebook.com/pages/Dectris-Ltd/623855944369304> >> <https://twitter.com/DECTRIS_News> >> >> >> >> >> >> Confidentiality Note: This message is intended only for the use of the >> named >> recipient(s) and may contain confidential and/or privileged information. 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