Dear all I have got my se-phase structure with ccp4-CRANK2-SAD, and its Rfree is 0.26 when CRANK2 output, but the Rfree is higher to 0.36 when I refine it with refmac or phenix-refinement. does it mean I got a wrong phase, or I didn't got the right parameters when refinement.BTW, the resolution is 2.1A.THANKS A LOT
Best Regards shijun
