Not quite what you were looking for, but there was just that paper from Alexander McPherson abut dye binding. They show that some of the dyes seem to bind really tight to the protein, but are invisible in the structure. Maybe there are also some interesting references in there. Sorry haven't got around to read through it by myself, except for the abstract so far. Cheers Christian
Investigation into the binding of dyes within protein crystals. <https://www.ncbi.nlm.nih.gov/pubmed/30198893> *McPherson A*, Larson SB. Acta Crystallogr F Struct Biol Commun. 2018 Sep 1;74(Pt 9):593-602. doi: 10.1107/S2053230X18010300. Epub 2018 Sep 3. PMID:30198893 On Tue, Sep 11, 2018 at 1:42 PM Tobias Beck <tobiasb...@gmail.com> wrote: > Thanks for the replies I have received so far (on and off list). > > I would like to emphasize an important aspect: What about crystallization > trials where the ingredients do not show up, but cannot be omitted for the > crystallization, e.g. buffers to control pH, as mentioned in one response - > I am also thinking of specific ions, that are required, but are disordered > - any general reference for this? > > > Can't you just point out that crystallization is a purification method > Yes, that would be one way to put it. > > Thanks, Tobias. > > > On Tue, Sep 11, 2018 at 12:38 PM Patrick Shaw Stewart < > patr...@douglas.co.uk> wrote: > >> >> Can't you just point out that crystallization is a purification method? >> E.g. look at the number of proteins in the Sigma catalogue that have been >> purified by crystallization. Clearly crystallization often excludes other >> solutes in the mix. >> >> Best wishes, Patrick >> >> >> On Tue, 11 Sep 2018 at 08:41, Tobias Beck <tobiasb...@gmail.com> wrote: >> >>> Dear all, >>> >>> I am looking for some general references regarding the fact that for a >>> crystallization condition not all ingredients of the crystallization >>> cocktail will show up in the crystal structure. Ions could be disordered, >>> buffer components located in the solvent regions, etc. >>> >>> I think this is rather common knowledge, but maybe there is, especially >>> regarding protein crystallization, a more general reference (apart from >>> text books on solubility) for this and I am just not using the right search >>> terms... >>> >>> Thank you! >>> >>> Best, Tobias. >>> -- >>> ___________________________________________ >>> >>> Dr. Tobias Beck >>> - independent group leader - >>> RWTH Aachen University >>> Institute of Inorganic Chemistry >>> Landoltweg 1, office: 304N >>> 52056 Aachen, Germany >>> phone: +49-241-80-90057 >>> fax: +49-241-80-99003 >>> web: http://www.ac.rwth-aachen.de/extern/beck/ >>> ___________________________________________ >>> >>> >>> ------------------------------ >>> >>> To unsubscribe from the CCP4BB list, click the following link: >>> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 >>> >> >> >> -- >> patr...@douglas.co.uk Douglas Instruments Ltd. >> Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK >> Directors: Peter Baldock, Patrick Shaw Stewart >> >> http://www.douglas.co.uk >> Tel: 44 (0) 148-864-9090 US toll-free 1-877-225-2034 >> Regd. England 2177994, VAT Reg. GB 480 7371 36 >> > > > -- > ___________________________________________ > > Dr. Tobias Beck > - independent group leader - > RWTH Aachen University > Institute of Inorganic Chemistry > Landoltweg 1, office: 304N > 52056 Aachen, Germany > phone: +49-241-80-90057 > fax: +49-241-80-99003 > web: http://www.ac.rwth-aachen.de/extern/beck/ > ___________________________________________ > > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
