To add: I think what Dr. Garman means by "match" is not necessarily to
set the two flow rates to the same value. The optimal settings will
depend on the size, shape, and even orientation of your nozzle. You
need to fiddle with the outer stream flow rate to find the one that
minimizes the turbulence.
Mounting up a large, long-necked nylon loop with a big drop of liquid in
it makes an excellent vibrometer. You can often see the loop vibration
under the video microscope if your frame rate is high enough. And, of
course, the most systematic, sensitive and relevant assay is to put a
crystal in it and collect some data:
https://doi.org/10.1107/S0021889808032536
-James Holton
MAD Scientist
On 7/3/2020 3:09 AM, Elspeth Garman wrote:
Yes, if you don’t match the inner and outer stream rates you get
turbulent flow at the boundary between them and ice build up on the
sample.
Best wishes
Elspeth
*From:*CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> *On Behalf Of
*Marcus Winter
*Sent:* 03 July 2020 09:29
*To:* CCP4BB@JISCMAIL.AC.UK
*Subject:* [ccp4bb] flow rate for cooling stream?
Dear Patrick, Herman,
Regarding the Cryostream, then referring to the Cryostream 800
brochure indicates that the N2 (gas) flow rates employed are in the
range of 5 - 10 */litres/* / minute, … and, I guess, therefore
something similar for the outer dry air 'shield' stream. Obviously,
it’s best to check directly with the manufacturers (Oxford
Cryosystems) directly on the details.
All The Best,
Marcus Winter
(Rigaku)
-----Original Message-----
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
Schreuder, Herman /DE
Sent: Friday, July 03, 2020 7:44 AM
To: CCP4BB@JISCMAIL.AC.UK <mailto:CCP4BB@JISCMAIL.AC.UK>
Subject: [ccp4bb] AW: [EXTERNAL] [ccp4bb] flow rate for cooling stream?
Dear Patrick,
if I recall correctly, our systems run at 10-15 ml/min (gas). I will
check on Monday when I am back in the lab.
The original cryostreams would run for several day's on a tank of
liquid nitrogen. However, they had significant hardware to dry the
nitrogen and to ensure a constant flow.
Best, Herman
-----Ursprüngliche Nachricht-----
Von: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK
<mailto:CCP4BB@JISCMAIL.AC.UK>> Im Auftrag von Patrick Loll
Gesendet: Donnerstag, 2. Juli 2020 22:03
An: CCP4BB@JISCMAIL.AC.UK <mailto:CCP4BB@JISCMAIL.AC.UK>
Betreff: [EXTERNAL] [ccp4bb] flow rate for cooling stream?
EXTERNAL : Real sender is owner-ccp...@jiscmail.ac.uk
<mailto:owner-ccp...@jiscmail.ac.uk>
Sorry, way off topic:
Does anyone have an estimate for the flow rate one would typically use
for the cold nitrogen stream passing over a protein crystal in a
standard data collection?
Background: Our nitrogen “generator” has gone belly-up and the vendor
no longer services it, so I’m testing the feasibility of using the
boil-off from a liquid nitrogen tank to provide the gas to support a
short data collection (this nitrogen gas would serve as the feedstock
into our helium cryostat). But I don’t know the flow rate required, so
I can’t calculate if one tank has enough nitrogen to support a day or
so of data collection. There are flow meters for the warm and cold
stream on the nitrogen generator, but these flow meters have no
apparent units anywhere on them, so I have no idea of the rate at
which the gas would be consumed.
Thanks for any useful tidbits.
And for those of you in the US, best wishes for a happy “Holy crap,
even MORE fireworks?!?!!” Day
Pat
---------------------------------------------------------------------------------------
Patrick J. Loll, Ph. D.
Professor of Biochemistry & Molecular Biology Drexel University
College of Medicine Room 10-102 New College Building
245 N. 15th St., Mailstop 497
Philadelphia, PA 19102 USA
(215) 762-7706
pjl...@gmail.com <mailto:pjl...@gmail.com>
pj...@drexel.edu <mailto:pj...@drexel.edu>
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