What about proteins where the crystals don't diffract well? I've found
in my teaching experience that students take your advice much more
seriously when they see it work on 3.5A data than they do when they see
it work on 1.5 A data.
-James Holton
MAD Scientist
On 6/17/2021 7:40 AM, Tanner, John J. wrote:
We developed a senior undergraduate biochemistry lab around an acid
phosphatase from Francisella tularensis (FtHAP).
https://pubmed.ncbi.nlm.nih.gov/27980518/
<https://pubmed.ncbi.nlm.nih.gov/27980518/>
The enzyme is easy to purify and crystallize, and the crystals
diffract well. L-tartrate and phosphate ion are inexpensive
inhibitors, which can be included in crystallization (3IT0/1). We have
the students grow crystals and collect X-ray diffraction data
remotely. Then they view maps in Coot. The enzyme assay is a simple
colorimetric test with p-nitrophenylphosphate as the substrate.
--
John J. Tanner
Professor of Biochemistry and Chemistry
Associate Chair of Biochemistry
Department of Biochemistry
University of Missouri
117 Schweitzer Hall
503 S College Avenue
Columbia, MO 65211
Phone: 573-884-1280
Email: tanne...@missouri.edu <mailto:tanne...@missouri.edu>
https://cafnrfaculty.missouri.edu/tannerlab/
<https://cafnrfaculty.missouri.edu/tannerlab/>
Lab: Schlundt Annex rooms 3,6,9, 203B, 203C
Office: Schlundt Annex 203A
*From: *CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> on behalf of P. H
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*Date: *Wednesday, June 16, 2021 at 5:19 PM
*To: *CCP4BB@JISCMAIL.AC.UK <CCP4BB@JISCMAIL.AC.UK>
*Subject: *[ccp4bb] Looking for proteins for undergraduate
biochemistry lab
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Hello All,
We are looking for some candidate proteins for an undergraduate level
advanced biochemistry lab. They should be expressed in bacteria,
simple enough to purify and it will be nice to perform some simple
characterization experiments(binding assays, enzymatic assays).
Any suggestions?
Thank you in advance.
Prerna gupta
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