Nb, dissolution of BeCl2 in water is quite exothermic and releases HCl vapor, you will want to prepare that stock in a fume hood.
Best wishes Kevin From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> on behalf of Matthew BOWLER <mbow...@embl.fr> Date: Thursday, January 4, 2024 at 2:19 AM To: CCP4BB@JISCMAIL.AC.UK <CCP4BB@JISCMAIL.AC.UK> Subject: Re: [ccp4bb] How to make GDP.BeF3 solution ? Dear Firdous, beryllium fluoride is actually a ground state analogue of GTP as trifluoroberyllate is tetrahedral. To get a transition sate analogue you need either AlF4- or MgF3-. Preparation of these complexes is very easy. The great advantage of metal fluoride transition state analogue and ground state analogue complexes is the fact that all components are present in solution and readily self-assemble in the active site forming stable enzyme complexes that are relevant to the catalytic cycle. The inorganic metal fluoride salts (AlF3, and MgF2) are too insoluble to use; therefore, the fluoride anion and metal cation components must be added from separate stock solutions. Both ammonium fluoride and sodium fluoride are suitable as the source of fluoride and are readily soluble in water. Metal chlorides can be easily dissolved in water at high concentration (0.5 M) and the solutions conserved at -20°C. One of the critical aspects in preparing metal fluoride enzyme complexes is the pH of the resulting solution. In particular, solutions of AlCl3 and BeCl2 are highly acidic (pH 2) samples should be prepared in 100 mM unbuffered Tris base. The optimized sequence of component addition is to add fluoride to the prepared buffer first, then the metal chloride stock. Hope this helps, Matt On 02/01/2024 18:53, Firdous Tarique wrote: Hi I would appreciate it if someone could share with me a step by step protocol for making a stable GDP.BeF3 solution which is often used as a transition state analogue for structural studies of a protein complex ? Or a vendor from where it can be purchased directly. Regards Firdous ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 -- Matthew Bowler Project Leader MASSIF1@ESRF European Molecular Biology Laboratory 71 avenue des Martyrs CS 90181 F-38042 Grenoble France =================================================== Tel: +33 (0) 4.76.20.76.37 Fax: +33 (0) 4.76.20.71.99 http://www.embl.fr/ http://www.esrf.eu/MASSIF1 https://twitter.com/id30_massif1 https://www.embl.org/people/person/mbowler/ =================================================== ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
