Don't forget to try pure water.
On 05/02/2024 10:44, Savvas Savvides wrote:
Dear Kavya,
we encountered this issue for a protein complex at 95 mg/mL involving
Human Serum Albumin (HSA) and a designed protein binder (Alphabody) as
described in Pannecoucke et al. 2021 Sci Adv 7 (13),
DOI:10.1126/sciadv.abe1682.
The breakthrough in that case came from crystallization experiments at
different temperatures (14C, 21C and 37C), with 37C coming out as the
clear winner.
best wishes
Savvas
——
Prof. Savvas Savvides
VIB Center for Inflammation Research
Ghent University, Dept. of Biochemistry & Microbiology
Technologiepark 71, 9052 Ghent, Belgium
Email: savvas.savvi...@ugent.be ; savvas.savvi...@irc.vib-ugent.be
Phone: +32 (0)472 928 519 (mobile) ; +32 (0)9 331 36 60 (office)
Web:_https://www.irc.ugent.be/index.php/groups/savvides-unit_
On 5 Feb 2024, at 11:27, kavyashreem <kavyashr...@instem.res.in> wrote:
Dear All,
Has anyone worked on a protein which is highly soluble even at 80mg/ml?
We have one such candidate, which does not precipitate even at
80mg/ml instead forms phase separated globules in crystallization
plate, which eventually hardens over a period of 1 to 1.5 months
(which is florescent under UV microscope.)
We tried screening at different pH, but failed to get any hits.
Since we got few conditions in which the phase separated globules
solidified, we focused on them and expanded with 120mg/ml protein,
still there were not visible precipitates except for the phase
separation. This has been a challenging target so far. We have tried
with different constructs, which unfortunately are not soluble!
Does POMs help in such cases? Or do you have any other suggestion.
Thank you
Regards
Kavya
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