Briefly, this is not the way that Coot wants you to do it. Coot wants you to "Replace Residue" (under modelling). Make sure that your restraints for the modified residue have the group DNA (or RNA), not monomer.
Paul. ________________________________________ From: Mailing list for users of COOT Crystallographic Software [c...@jiscmail.ac.uk] On Behalf Of Mary Varughese [elizabethvj1...@gmail.com] Sent: 02 June 2010 11:19 To: COOT@JISCMAIL.AC.UK Subject: To mutate non-standard nucleic acids We are working on modified nucleic acids. Using a pdb file of a modified nucleic acid, I want to cut and change the positions of the residues in the nucleic acid chain. I tried it with calculate--> model/fit/refine-->rotate/translate zone, although we cut and place it at the correct distance no bond is forming. Is there any way i can do this?
