-Caveat Lector-

Dave Hartley
http://www.Asheville-Computer.com/dave

===================

Date: Wed, 5 Jan 2000 16:29:13
From: j.e. cummins <[EMAIL PROTECTED]>


The GM bacteria used to "purify" radioactive waste sites cause ionic
mercury to be released as volatile mercury metal. Such bacteria have
probably escaped into the environment to convert more ionic mercury in
geologic strata. Such mercury released has begun to cause major problems
in cold places like Canada, Alaska, and the northern continental states.
The Arctic is facing tragic injury to the top of the food chain including
people, polar bears, beluga, narwhal,walrus, etc. Those victims have begun
to show birth defects and nerve damage from mercury in their only source
of food. The question is" who in the hell told the US department of energy
that it was O.K.?
 ======================

Articles Nature Biotechnology

January 2000 Volume 18 Number 1 pp 85 - 90



Hassan Brim n.1, Sara C. McFarlan n.2, James K. Fredrickson n.3, Kenneth
W. Minton n.1, Min Zhai n.1, Lawrence P. Wackett n.2 & Michael J. Daly n.1

 1. Department of Pathology, Uniformed Services University of the Health
Sciences, Bethesda, MD 20814.
 2. Department of Biochemistry, Biological Process Technology Institute
and Center for Biodegradation Research and Informatics, Gortner
Laboratory, University of Minnesota, St. Paul, MN 55108.
 3. Pacific Northwest National Laboratory, Richland, WA 99352.

Correspondence should be addressed to M J Daly.
e-mail:[EMAIL PROTECTED]


We have developed a radiation resistant bacterium for the treatment of
mixed radioactive wastes containing ionic mercury. The high cost of
remediating radioactive waste sites from nuclear weapons production has
stimulated the development of bioremediation strategies using Deinococcus
radiodurans, the most radiation resistant organism known. As a frequent
constituent of these sites is the highly toxic ionic mercury (Hg) (II), we
have generated several D. radiodurans strains expressing the cloned Hg
(II) resistance gene (merA) from Escherichia coli strain BL308. We
designed four different expression vectors for this purpose, and compared
the relative advantages of each. The strains were shown to grow in the
presence of both radiation and ionic mercury at concentrations well above
those found in radioactive waste sites, and to effectively reduce Hg (II)
to the less toxic volatile elemental mercury. We also demonstrated that
different gene clusters could be used to engineer D. radiodurans for
treatment of mixed radioactive wastes by developing a strain to detoxify
both mercury and toluene. These expression systems could provide models to
guide future D. radiodurans engineering efforts aimed at integrating
several remediation functions into a single host.



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