Hi, I have spent too many hours trying to get eprimer3 to work on my Mac, and am out of ideas.
I installed primer3 2.3.5 on Mac. I can run it successfulyl with command line. When I try and run eprimer3 I get errors. >From the emboss README: ... primer3 from the Whitehead institute must be installed in one or two versions. EMBOSS application eprimer3 launches primer3_core and expects this to be version 1.x of the program. EMBOSS application eprimer32 launches primer32_core and expects this to be a renamed primer3_core from version 2.x of the program. primer3's configuration files also need to be installed. The original application looks by default in /opt/primer3_config ... $ eprimer32 -sequence=AAAA Pick PCR primers and hybridization oligos Died: eprimer32 uses external program 'primer32_core' which is not in the PATH or defined as EMBOSS_PRIMER32_CORE Part of the 'primer3' package, version 2.2.3, available from the Whitehead Institute. See: http://primer3.sourceforge.net/ The primer3_core application must be renamed to primer32_core ... This suggested to me that emboss expects primer32_core to be named primer32_core, not primer3_core as indicated above. renaming the program to primer32_core, or typing eprimer3 into the command line produce the same error: ... $ eprimer3 -sequence=AAAA Pick PCR primers and hybridization oligos Error: Failed to open filename 'AAAA' Error: Unable to read sequence 'AAAA' Died: eprimer3 terminated: Bad value for '-sequence' and no prompt .... I get this error regardless of what I put as the sequence, or even if I input a file. Does anyone have suggestions or potential diagnostics? Thanks Mark _______________________________________________ EMBOSS mailing list EMBOSS@lists.open-bio.org http://lists.open-bio.org/mailman/listinfo/emboss