Hi Guy, in the current EMBOSS there are 2 wrappers for eprimer: #### eprimer3 Pick PCR primers and hybridization oligos eprimer32 Pick PCR primers and hybridization oligos ####
eprimer3 is for primer3 version 2.3.x (works for me with 2.3.6) and eprimer32 is for the older version 2.2.3 So if you want to use the new primer3 version (2.3.5), the symbolic link is not needed. Just put primer3_core in the path and use eprimer3. Kind regards, David. -----Ursprüngliche Nachricht----- Von: emboss-boun...@lists.open-bio.org [mailto:emboss-boun...@lists.open-bio.org] Im Auftrag von Guy Bottu Gesendet: 13 February 2014 18:17 An: emboss@lists.open-bio.org Betreff: [EMBOSS] problem with eprimer32 Dear all, I installed on my computer EMBOSS version 6.6.0.0 and tried to make eprimer32 work. I installed the last version of Primer3 (version 2.3.5) and I put a logical link in the bin directory of EMBOSS (primer32_core -> .../primer3_core). When I try to run it, I get : Pick PCR primers and hybridization oligos Whitehead primer3_core program output file [emboss_001.eprimer32]: Error: thermodynamic approach chosen, but path to thermodynamic parameters not specified What could be missing ? The EMBOSS eprimer32 manual does not say anything beyond the need to have eprimer3_core in the path. Regards, Guy Bottu --- Dit e-mailbericht bevat geen virussen en malware omdat avast! Antivirus-bescherming actief is. http://www.avast.com _______________________________________________ EMBOSS mailing list EMBOSS@lists.open-bio.org http://lists.open-bio.org/mailman/listinfo/emboss _______________________________________________ EMBOSS mailing list EMBOSS@lists.open-bio.org http://lists.open-bio.org/mailman/listinfo/emboss
