Put some alcohol in the water bath - it will reduce the surface tension. Maybe that will help. j
-----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of John Shelley Sent: Tuesday, November 10, 2009 09:25 To: histonet@lists.utsouthwestern.edu Cc: Betsy Woessner; Kathryn Gizzi Subject: [Histonet] Tissue falling off slides Good morning histonetters!! I received a project from an outside source to process and embed mouse hearts for this group. From what I could gather the specimens had varying times in 4% paraformaldehyde from 24-48 hours all the way up to 4 months. Once processed and embedded the specimens where returned to the group to cut. They are using superfrost plus slides from Fisherbrand and from what I could see the sections were coming off fine. The only thing I did notice is that when they put the slide in the water bath the tissue seemed to be repelled which from experience means that too much coating on slide and a hydrophobic environment is create. I asked them to look for slides from a different lot and cut additional sections and from those sections I asked them to air-dry only and others I asked for them to place in oven for 30-45 mins. at 56 to 60 degrees or until they did not see any more water droplets. They said the sections that they placed in the oven stayed on a little longer in the deparaffinizing steps but still fell off. I know that it could be processing also but the specimens looked great when I embedded them. Any suggestions will be great, sometimes when your right on top of it you lose sight of other possibilities. John _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet