Hi I used to have that problem. A senior histologist from BioCare recommended to air dry the slides overnight and then bake them. I have not had the problem since. Rachel
From: Ken M via Histonet <histonet@lists.utsouthwestern.edu> Sent: Wednesday, January 31, 2024 6:07 PM To: richard cartun <rcar...@sbcglobal.net>; histonet@lists.utsouthwestern.edu; Foshey, Annette <afos...@mcw.edu> Subject: Re: [Histonet] Tissue falling off slides 1) Try tap water 2) Try hydrophilic IHC slides, (I will send you samples for free) Ken kdea...@hotmail.com<mailto:kdea...@hotmail.com> -----Original Message----- From: richard cartun via Histonet <histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu>> Sent: Wednesday, January 31, 2024 10:29 AM To: histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu>; Foshey, Annette <afos...@mcw.edu<mailto:afos...@mcw.edu>> Subject: Re: [Histonet] Tissue falling off slides Hi Annette! Do you put anything else in your water bath besides deionized water? Richard CartunMorphologic Proteomics, LLC(860) 490-7633 On Tuesday, January 30, 2024 at 03:14:50 PM EST, Foshey, Annette via Histonet <histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu>> wrote: I work with whole mount prostate blocks that I cut and stain for IHC manually. I use superfrost plus slides that I purchase from the Brain Research Laboratories. I airdry the slides after sectioning and then they are placed in a rack and dried in a 60 degree oven overnight. On some of the slides the tissue still lifts off the slides usually it is where there is tumor. Any suggestions would be greatly appreciated. Kindest Regards, Annette Foshey, HT Histology Technologist, Precision Medicine Laboratory (PML) Phone: (414) 955-7537 | E-mail: afos...@mcw.edu<mailto:afos...@mcw.edu<mailto:afos...@mcw.edu%3cmailto:afos...@mcw.edu>> Medical College of Wisconsin | 8701 W Watertown Plank Road, Milwaukee, WI 53226 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu<mailto:Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet<http://lists.utsouthwestern.edu/mailman/listinfo/histonet> _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu<mailto:Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet<http://lists.utsouthwestern.edu/mailman/listinfo/histonet> _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu<mailto:Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet<http://lists.utsouthwestern.edu/mailman/listinfo/histonet> Disclaimer The information contained in this communication from the sender is confidential. It is intended solely for use by the recipient and others authorized to receive it. If you are not the recipient, you are hereby notified that any disclosure, copying, distribution or taking action in relation of the contents of this information is strictly prohibited and may be unlawful. This email has been scanned for viruses and malware, and may have been automatically archived by Mimecast Ltd, an innovator in Software as a Service (SaaS) for business. Providing a safer and more useful place for your human generated data. Specializing in; Security, archiving and compliance. To find out more visit the Mimecast website. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
