-----BEGIN PGP SIGNED MESSAGE----- Hash: SHA1 >> So the question is: if I ask for 5'UTR+upstream-800, shouldn't I >> always be getting at least 800 bp of upstream sequence, irrespective >> of the absence/presence of the 5'UTR?
The upstream/downstream generator adds extra bases relative to the start/end of the feature selected (5'UTR, 3'UTR, gene flank, etc.). If that feature does not exist, then there is no coordinate to start taking extra bases from, and so you can't get additional sequence. cheers, Richard -----BEGIN PGP SIGNATURE----- Version: GnuPG v1.4.2.2 (GNU/Linux) Comment: Using GnuPG with Mozilla - http://enigmail.mozdev.org iD8DBQFGjOUd4C5LeMEKA/QRAkjJAJ9E3B+BCpTUQw0rAo9WKAQW4OMsHgCfTgR3 ib09Bb2sjzi7cbH+iM5doyI= =H8RE -----END PGP SIGNATURE-----