I follow the method outlined by Nick with one additional step, after selecting the new residue you can run through a sidechain rotamer library using the forward arrows at the bottom of the window. You also have a choice to use backbone dependant or backbone independant rotamers.
Andrew On 4/18/06, Noinaj <noi...@uky.edu> wrote: > > Orla, > > This is simply, for the most part. Here is what I do. > > Load your *.pdb file. Then under WIZARD in the menu bar, select > MUTAGENESIS. Then pretty much, follow directions. In the PyMOL VIEWER > window, it will ask you to ' pick a residue.' Once you pick a residue, > select the type of residue you want to mutate to by left clicking on [No > Mutation] button and holding down (under Mutagenesis). This brings up a > list of residues that you can then select from. Once you select the residue > you want, hit APPLY, then DONE. If everything was done correctly, you > should see your mutated residue. > > There are probably other ways to do this, but I hope this helps. If you > would like screenshots of the steps, just let me know. > > > > Cheers, > Nick > > > > > ----- Original Message ----- > From: Orla O'Sullivan > To: pymol-users@lists.sourceforge.net > Sent: Tuesday, April 18, 2006 11:31 AM > Subject: [PyMOL] mutations > > > > > Hi all > > I'm a newbie to Pymol and so far have been very impressed. However I have > tried to mutate some residues in my structure and have hit a brick wall. > > Has anyone done this with pymol and let me know how to do it? > > > > Regards > > > > Orla > > > > Dr.Orla O'Sullivan > > Research Officer > > Biotechnology > > > > > > Moorepark Food Research Centre > Teagasc > > Moorepark > Fermoy, Co. Cork > Ireland > > > > > Tel: +353 - (0)25 - 42344 > >
