Well, since 1000 are a sample, 100 sampled from those is a somewhat more
manageable sample.

As to using, say, an 80% cutoff is a valid procedure, one question is what
you're doing this for. I'd be tempted to just show or report the
distribution of K, lambda, and p values and talk about what they mean in
terms of your biological question: "We don't know the tree exactly, but
it's clear that for most feasible trees, related things are similar" or
something like that. If it's a question of scaling trees for later
analyses, you can just (using a script, or built in functions) choose the
particular scaling to be appropriate for each tree, and so the distribution
across them doesn't matter in that case.

Best,
Brian

_______________________________________________________________________
Brian O'Meara, http://www.brianomeara.info, especially Calendar
<http://brianomeara.info/calendars/omeara/>, CV
<http://brianomeara.info/cv/>, and Feedback
<http://brianomeara.info/teaching/feedback/>

Associate Professor, Dept. of Ecology & Evolutionary Biology, UT Knoxville
Associate Head, Dept. of Ecology & Evolutionary Biology, UT Knoxville
Associate Director for Postdoctoral Activities, National Institute for
Mathematical & Biological Synthesis <http://www.nimbios.org> (NIMBioS)
Communication Director, Society of Systematic Biologists

On Thu, Dec 8, 2016 at 11:26 AM, Carturan, Bruno <bruno.cartu...@ubc.ca>
wrote:

> Hello R-sig-phylo community,
>
> I am trying to measure the phylogenetic signal of functional traits with
> the Blomberg’s K and Pagel’s λ methods. My problem is that I don’t have one
> phylogenetic tree but 1000 (the trees were created by first combining
> different molecular, morphologic and taxonomic trees, then by conducting a
> MCMC analysis and finally by selecting the 1000 trees – work made by Huang
> and Roy 2015).
>
> Is there a way to proceed to test the significance of the K and λ values
> for each trait with these 1000 trees?
>
> An idea I have is to run the test and obtain a p value for each of the
> 1000 trees, which would give me a distribution of p values, and then
> consider that if 80% (for instance) of the p values are significant, the K
> or λ value is significant. This is computationally very demanding (I have
> 735 species) and I don’t know if this could be considered as a valid
> procedure.
>
> I am looking forward for you answer.
>
> Bruno Carturan
> PhD Candidate
> Complex Environmental Systems Lab
> University of British Columbia
> Okanagan Campus
>
>
> ________________________________________
> From: Liam J. Revell [liam.rev...@umb.edu]
> Sent: Wednesday, December 07, 2016 6:54 AM
> To: Carturan, Bruno
> Subject: Re: A question about phylogenetic signal significance with 1000
> trees
>
> Bruno.
>
> This is an interesting question. Since this doesn't pertain specifically
> to phytools, perhaps you should pose it to the R-sig-phylo email
> list-serve. You may get a good answer there.
>
> All the best,
>
> Liam J. Revell, Associate Professor of Biology
> University of Massachusetts Boston
> web: http://faculty.umb.edu/liam.revell/
> email: liam.rev...@umb.edu
> blog: http://blog.phytools.org
>
> On 12/6/2016 6:16 PM, Carturan, Bruno wrote:
> > Hello Dr. Revell,
> >
> >
> >
> > I am a PhD student from the Complex Environmental Systems Lab, from the
> > University of British Columbia and I would like to ask you question.
> >
> >
> >
> > I am trying to measure the phylogenetic signal of functional traits with
> > the Blomberg’s K and Pagel’s λ methods. My problem is that I don’t have
> > one phylogenetic tree but 1000 (the trees were created by first
> > combining different molecular, morphologic and taxonomic trees, then by
> > conducting a MCMC analysis and finally by selecting the 1000 trees –
> > work made by Huang and Roy 2015).
> >
> >
> >
> > Is there a way to proceed to test the significance of the K and λ values
> > for each trait with these 1000 trees?
> >
> >
> >
> > An idea I have is to run the test and obtain a p value for each of the
> > 1000 trees, which would give me a distribution of p values, and then
> > consider that if 80% (for instance) of the p values are significant, the
> > K or λ value is significant. This is computationally very demanding (I
> > have 735 species) and I don’t know if this could be considered as a
> > valid procedure.
> >
> >
> >
> > I am looking forward for you answer.
> >
> >
> >
> > Best regards,
> >
> >
> > Bruno Carturan
> > PhD Candidate
> > Complex Environmental Systems Lab
> > University of British Columbia
> > Okanagan Campus
> > <http://www.ubc.ca/okanagan/>
>
> _______________________________________________
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> https://stat.ethz.ch/mailman/listinfo/r-sig-phylo
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