Re: [base] BASE 2 Illumina arrays

2007-07-31 Thread Nicklas Nordborg
Nicklas Nordborg wrote:
 Jeremy Davis-Turak wrote:
 I have implemented the incorporation of Illumina data into our BASE 
 system, along with a raw data importer.  Our version requires that the 
 gene_profile.csv be split up into separate files, which we have done 
 with an R script.  This can also be done manually in excel, or feasibly 
 in a BASE script. 

 Nicklas, how do I share the plugin configurations with others?
 
 I have created a ticket in our trac system for this issue:
 http://base.thep.lu.se/ticket/486
 
 Everyone are welcome to comment and it is also possible to attach files 
 to it. Note that you have to be logged in before you can comment or 
 upload files. Use base/base as username/password.
 
 I think the best solution would be to have a BASE plugin that can split 
 the file automatically and the import the raw data in one go. If that is 
 possible or not I don't know since I have no knowledge of the file format.

We have planned to include the Illumina import plug-in in the next 
release (2.4). We have not been able to find any good description of the 
file format or example data files. Does anybody out there have any 
information to help us implement this?

We definitely need one or more example data files that we can use for 
testing. The files will be put in a protected repository that is only 
available to the core developers. It would also be nice to have a more 
formal description, or at least a short overview, of the file format.

Since it is only 3 weeks to go before 2.4 is released we need any 
information as soon as possible (this week!).

/Nicklas

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[base] svn check out of BASE2 WS enabled

2007-07-31 Thread Virginie Garcia
Hi all,

I try to download to webservices enabled version of BASE with svn as 
mentioned on the plugins page 
(http://baseplugins.thep.lu.se/wiki/se.lu.thep.webservices_clients).
When I check out the web services branch (svn co 
http://lev.thep.lu.se/repository/base/branches/webservices 
BASE-webservices) , I always get the same error :

svn: REPORT request failed on '/repository/base/!svn/vcc/default'
svn: REPORT of '/repository/base/!svn/vcc/default': 400 Bad Request 
(http://lev.thep.lu.se)

Does anyboby have already tried to get this version ?

Thanks

Virginie


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Re: [base] svn check out of BASE2 WS enabled

2007-07-31 Thread Nicklas Nordborg
Virginie Garcia wrote:
 Hi all,
 
 I try to download to webservices enabled version of BASE with svn as 
 mentioned on the plugins page 
 (http://baseplugins.thep.lu.se/wiki/se.lu.thep.webservices_clients).
 When I check out the web services branch (svn co 
 http://lev.thep.lu.se/repository/base/branches/webservices 
 BASE-webservices) , I always get the same error :
 
 svn: REPORT request failed on '/repository/base/!svn/vcc/default'
 svn: REPORT of '/repository/base/!svn/vcc/default': 400 Bad Request 
 (http://lev.thep.lu.se)
 
 Does anyboby have already tried to get this version ?

I just tried it and it works for me. I searched the net for similar 
problems and most of them seemed to be related to the user beeing behind 
a proxy that doesn't pass on all types of requests. Check for example 
this link:
http://lists.ipython.scipy.org/pipermail/ipython-dev/2005-September/001737.html

A suggested workaround is to change the http:// to https://

/Nicklas

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Re: [base] svn check out of BASE2 WS enabled

2007-07-31 Thread Virginie Garcia
OK. It works fine with https.

Thanks a lot.

Virginie


Nicklas Nordborg a écrit :
 Virginie Garcia wrote:
   
 Hi all,

 I try to download to webservices enabled version of BASE with svn as 
 mentioned on the plugins page 
 (http://baseplugins.thep.lu.se/wiki/se.lu.thep.webservices_clients).
 When I check out the web services branch (svn co 
 http://lev.thep.lu.se/repository/base/branches/webservices 
 BASE-webservices) , I always get the same error :

 svn: REPORT request failed on '/repository/base/!svn/vcc/default'
 svn: REPORT of '/repository/base/!svn/vcc/default': 400 Bad Request 
 (http://lev.thep.lu.se)

 Does anyboby have already tried to get this version ?
 

 I just tried it and it works for me. I searched the net for similar 
 problems and most of them seemed to be related to the user beeing behind 
 a proxy that doesn't pass on all types of requests. Check for example 
 this link:
 http://lists.ipython.scipy.org/pipermail/ipython-dev/2005-September/001737.html

 A suggested workaround is to change the http:// to https://

 /Nicklas

   

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Re: [base] BASE 2 Illumina arrays

2007-07-31 Thread Jeremy Davis-Turak
Hi Nicklas,

I uploaded some data files to the Ticket.

Here is a brief summary of what the data looks like:

1)  Annotation data: CSV file.  It's too bad that it's a CSV, because
some of the fields contain commas!

2)  Data: (header is on ~ line 8)
a) For each set of chips that are processed at the same time, there is
one resulting file.  Thus, if you did two rat chips (each of which has
12 arrays on them), you would have 24 arrays contained in one file.
b) Depending on the settings of the software at the time of scanning,
you can have somewhere from 1-8 data columns per array (I don't know
the exact range, but I know that it's variable).
c)  The first column contains the probe IDs, the rest of them are data.
d) Each data column name is a concatenation of 3 things:
   i)  The data type (i.e. 'AVG_Signal' or 'BEAD_STDEV')
  ii) The chip number (10 digits)
   iii) A capital letter indicating the position of the array on the
chip (i.e. A-F for human, A-H for mouse, or A-L  for rat.)
   EXAMPLE: the first 8 columns in my rat file are:

AVG_Signal-1677718123_A
BEAD_STDEV-1677718123_A
Avg_NBEADS-1677718123_A 
Detection-1677718123_A  
AVG_Signal-1677718123_B 
BEAD_STDEV-1677718123_B 
Avg_NBEADS-1677718123_B 
Detection-1677718123_B

 and a number of columns later, they transition smoothly to the next chip:

Avg_NBEADS-1677718123_L 
Detection-1677718123_L  
AVG_Signal-1677718142_A 
BEAD_STDEV-1677718142_A 


In my R script, you have to hard-code the number of data columns per
array, and the number of arrays per chip.

Thanks for making this plugin!

Jeremy



On 7/31/07, Nicklas Nordborg [EMAIL PROTECTED] wrote:
 Nicklas Nordborg wrote:
  Jeremy Davis-Turak wrote:
  I have implemented the incorporation of Illumina data into our BASE
  system, along with a raw data importer.  Our version requires that the
  gene_profile.csv be split up into separate files, which we have done
  with an R script.  This can also be done manually in excel, or feasibly
  in a BASE script.
 
  Nicklas, how do I share the plugin configurations with others?
 
  I have created a ticket in our trac system for this issue:
  http://base.thep.lu.se/ticket/486
 
  Everyone are welcome to comment and it is also possible to attach files
  to it. Note that you have to be logged in before you can comment or
  upload files. Use base/base as username/password.
 
  I think the best solution would be to have a BASE plugin that can split
  the file automatically and the import the raw data in one go. If that is
  possible or not I don't know since I have no knowledge of the file format.

 We have planned to include the Illumina import plug-in in the next
 release (2.4). We have not been able to find any good description of the
 file format or example data files. Does anybody out there have any
 information to help us implement this?

 We definitely need one or more example data files that we can use for
 testing. The files will be put in a protected repository that is only
 available to the core developers. It would also be nice to have a more
 formal description, or at least a short overview, of the file format.

 Since it is only 3 weeks to go before 2.4 is released we need any
 information as soon as possible (this week!).

 /Nicklas

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 basedb-users@lists.sourceforge.net
 unsubscribe: send a mail with subject unsubscribe to
 [EMAIL PROTECTED]


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