[ccp4bb] Announcement of the Sixth International Workshop on X-ray Radiation Damage to Biological,Crystalline Samples. SSRL 11-13th March 2010.
The Sixth International Workshop on X-ray Radiation Damage to Biological Crystalline Samples will be held at the Stanford Synchrotron Radiation Laboratory from 13:00 March 11th to 13:00 March 13th 2010. This series of workshops was originally concerned with the effects of radiation damage during investigation of protein structures by X-ray crystallography. Other techniques of structural biology are now being included to ensure greater information exchange. The workshop will therefore be of interest to all those using ionising radiation to examine biological structures at the molecular and cellular level. Topics under discussion will include: 1: Reducing and Mitigating Radiation Damage 2: Radiation chemistry and radiolysis of biological molecules 3: Progress in the understanding of radiation damage in MX 4: Correcting for the effects of radiation damage 5: Reduction of metalloproteis and study of protein function 6: Radiation Damage in Electron Microscopy and Electron Crystallography 7: Pulsed Sources: Radiation damage issues. 8: Complementary (or non-crystallographic) methods to study radiation damage The organizers are Ana Gonzales, Elspeth Garman, Colin Nave, Sean McSweeney, Raimond Ravelli, Gerd Rosenbaum, Soichi Wakatsuki, and Martin Weik. Anyone who would like to participate should e-mail Elspeth Garman (elspeth.gar...@bioch.ox.ac.uk mailto:elsp...@biop.ox.ac.uk) who will forward further information to interested parties once it is available. -- **NOTE NEW E-MAIL ADDRESS AND PHONE NUMBER Elspeth F. Garman, President, British Crystallographic Association Professor of Molecular Biophysics, University of Oxford Postal address: Laboratory of Molecular Biophysics, Department of Biochemistry, University of Oxford, Tel: (44)-1865-613297 South Parks Road, FAX: (44)-1865-613201 OXFORD, OX1 3QU, U.K. E-mail: elspeth.gar...@bioch.ox.ac.uk http://www.biop.ox.ac.uk/www/garman/gindex.html -
[ccp4bb] Request for def.site for HKL2000 for ESRF-BM14 MAR 225 CCD
Dear All, I am looking for def.site file for HKL2000 used in ESRF-BM14 beam line. We collected the data using MAR 225 CCD detector. The following link does not seem to work for me. http://www.esrf.fr/exp_facilities/BM14/runexperiment/bm14-def.site We need this file to process our data in the home lab. If anybody has one, can you please share with us. Thanking you With regards S.Karthikeyan * Dr.Karthikeyan Subramanian, Ph.D Scientist Institute of Microbial Technology (CSIR) Sector 39-A, Chandigarh - 160036, INDIA email:skart...@imtech.res.in, skarthi...@gmail.com *
Re: [ccp4bb] Request for def.site for HKL2000 for ESRF-BM14 MAR 225 CCD
Dear All, I got the file. Thanks a lot for the same. I apologize for posting a 'non-CCP4' question to the CCP4BB. With regards S.Karthikeyan Dear All, I am looking for def.site file for HKL2000 used in ESRF-BM14 beam line. We collected the data using MAR 225 CCD detector. The following link does not seem to work for me. http://www.esrf.fr/exp_facilities/BM14/runexperiment/bm14-def.site We need this file to process our data in the home lab. If anybody has one, can you please share with us. Thanking you With regards S.Karthikeyan * Dr.Karthikeyan Subramanian, Ph.D Scientist Institute of Microbial Technology (CSIR) Sector 39-A, Chandigarh - 160036, INDIA email:skart...@imtech.res.in, skarthi...@gmail.com *
[ccp4bb] Linux flavour and hard disks
Dear List, I'm planning to migrate soon from Red Hat Linux 7.0 on an HP xw6000 workstation with dual Xeon processor. Please, any suggestion for the best Linux flavour to get the most out of today's crystallographic software? I've seen that both Ubuntu and Fedora are quite common. Also I'm in doubt about the following: will it be safer to use two mirror hard disks (as I'm doing now) or to use one HD for the software and one for the data? And, finally, please, what HD size is today most reasonable (big, but still fast enough)? Thanks a lot, Claudia Claudia Scotti Dipartimento di Medicina Sperimentale Sezione di Patologia Generale Universita' di Pavia Piazza Botta, 10 27100 Pavia Italia Tel. 0039 0382 986335/8/1 Facs 0039 0382 303673 _ More than messages–check out the rest of the Windows Live™. http://www.microsoft.com/windows/windowslive/
Re: [ccp4bb] Linux flavour and hard disks
I've used both Fedora (RH 9 through Fedora 8) and Ubuntu 9.04 for crystallogrpahy. Both are fine distros. Ubuntu was a little better working out of the box with all the hardware, and maintenance of the restricted Nvidia drivers is simpler. For some reason, Wine compatibility was better, too. Those are the principal reasons I switched from Fedora to Ubuntu. On the other hand, I had fewer problems with managing NFS shares with my Fedora boxes, but that may just be familiarity with the required commands. Both Fedora and Ubuntu seem to be supported by the major crystallographic software folks. Personally, I put the OS on a smaller HD, and the home directories for the lab on a larger HD. Data resides on a separate Linux server with a large HD. Currently, I'm running the OS on the master and client workstations on 200-300 Gbyte HDs (overkill--they are only using 4-30 Gbyte, you could go smaller), and the home directory is on a 400 Gbyte HD (about 30% full). The data server has a 500 Gbyte HD (and should probably be upgraded to 1 Tbyte). The home directories, data, and crystallographic software directories are all backed up to a backup server (500 Gbyte) daily via rsync, with the currrent and last three versions available. Quite frankly, HDs are so cheap right now, it doesn't cost much to install big drives for data storage. I could use RAID, but my system is pretty simple and seems to provide sufficient protection. Cheers. Claudia Scotti wrote: Dear List, I'm planning to migrate soon from Red Hat Linux 7.0 on an HP xw6000 workstation with dual Xeon processor. Please, any suggestion for the best Linux flavour to get the most out of today's crystallographic software? I've seen that both Ubuntu and Fedora are quite common. Also I'm in doubt about the following: will it be safer to use two mirror hard disks (as I'm doing now) or to use one HD for the software and one for the data? And, finally, please, what HD size is today most reasonable (big, but still fast enough)? Thanks a lot, Claudia Claudia Scotti Dipartimento di Medicina Sperimentale Sezione di Patologia Generale Universita' di Pavia Piazza Botta, 10 27100 Pavia Italia Tel. 0039 0382 986335/8/1 Facs 0039 0382 303673 check out the rest of the Windows Live™. More than mail–Windows Live™ goes way beyond your inbox. More than messages -- Roger S. Rowlett Professor Department of Chemistry Colgate University 13 Oak Drive Hamilton, NY 13346 tel: (315)-228-7245 ofc: (315)-228-7395 fax: (315)-228-7935 email: rrowl...@colgate.edu
Re: [ccp4bb] Linux flavour and hard disks
Dear Claudia, I use Ubuntu and I am happy with it, cannot say if it is the best. It is relatively easy to maintain which is what you want. As for HD, try and determine how much space a typical project would need and how many concurrent projects there might be. Data from images would be the most space consuming, software would probably not amount to much. This then gives you a minimum size of HD. The speed of the disk is perhaps less important because writing to disk is always slow. It is important to have enough memory so that programs can be held entirely in memory. Having to use swap space slows the whole machine. Disk partitions (of the same disk) is something worth discussing. If you have everything on one partition there is a danger that it will be filled and that this will impact on the performance. I always have the / partition separate which need only be 5-10GB, a /usr partition which could contain most of the software (30GB on the machine here) and a /home partition (for user accounts). You also need a swap partition, this should be 2 to 3 times the memory size. Maybe somebody knows the optimum size. The most important thing which is easily overlooked is backups. Having a mirrored disk or a RAID array is one way of reducing corrupted data. Hot swapable RAID arrays can protect you against disk failure. However if the building burns down and there is no offsite backup then you are screwed. Certainly having a second disk on which hourly/daily backups are done is a good idea. Getting some offsite backups, whether tapes or networked disks, is a must. Adam On Mon, 24 Aug 2009, Claudia Scotti wrote: Dear List, I'm planning to migrate soon from Red Hat Linux 7.0 on an HP xw6000 workstation with dual Xeon processor. Please, any suggestion for the best Linux flavour to get the most out of today's crystallographic software? I've seen that both Ubuntu and Fedora are quite common. Also I'm in doubt about the following: will it be safer to use two mirror hard disks (as I'm doing now) or to use one HD for the software and one for the data? And, finally, please, what HD size is today most reasonable (big, but still fast enough)? Thanks a lot, Claudia Claudia Scotti Dipartimento di Medicina Sperimentale Sezione di Patologia Generale Universita' di Pavia Piazza Botta, 10 27100 Pavia Italia Tel. 0039 0382 986335/8/1 Facs 0039 0382 303673 _ More than messages?check out the rest of the Windows Live?. http://www.microsoft.com/windows/windowslive/
Re: [ccp4bb] Linux flavour and hard disks
I've just set up two home computers for crystallographic work. I decided to play around, and set one up with Ubuntu and the other one with Fedora core 11. Both did OK but I'd say that I had to perform marginally less tweaking with Fedora than with Ubuntu. As far as disk space - you can cram together almost any number of hard drives using Logical Volume Management. And then there are numerous RAID versions - both hardware and software (my two cents: don't get involved in nerdy discussions of which one is 'better' - just get the one that is easier for you). As long as you're backed up it really does not matter which hard drive configuration you choose. I recommend running updates with whatever you like for software - and also cloning your hard drives using CloneZilla or any other tool so that in the event of system drive failure you can just literally swap a cloned drive in and your computer won't know the difference! Artem Dear List, I'm planning to migrate soon from Red Hat Linux 7.0 on an HP xw6000 workstation with dual Xeon processor. Please, any suggestion for the best Linux flavour to get the most out of today's crystallographic software? I've seen that both Ubuntu and Fedora are quite common. Also I'm in doubt about the following: will it be safer to use two mirror hard disks (as I'm doing now) or to use one HD for the software and one for the data? And, finally, please, what HD size is today most reasonable (big, but still fast enough)? Thanks a lot, Claudia Claudia Scotti Dipartimento di Medicina Sperimentale Sezione di Patologia Generale Universita' di Pavia Piazza Botta, 10 27100 Pavia Italia Tel. 0039 0382 986335/8/1 Facs 0039 0382 303673 _ More than messagescheck out the rest of the Windows Live. http://www.microsoft.com/windows/windowslive/
Re: [ccp4bb] Linux flavour and hard disks
On Aug 24, 2009, at 7:49 AM, ar...@xtals.org wrote: my two cents: don't get involved in nerdy discussions Here's a useful summary: http://bandcamp.tv/linux-demotivators/
Re: [ccp4bb] Linux flavour and hard disks
-Original Message- From: owner-ccp...@jiscmail.ac.uk [mailto:owner-ccp...@jiscmail.ac.uk] On Behalf Of Adam Ralph Sent: 24 August 2009 15:25 To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Linux flavour and hard disks You also need a swap partition, this should be 2 to 3 times the memory size. Maybe somebody knows the optimum size. Hi, here: https://help.ubuntu.com/community/SwapFaq it explains that 1 to 2 times memory size is optimal. However note that this calculation assumes you are going to use the hibernate to disk feature which allows you to restart the machine in exactly the state it was when you last hibernated (and which obviously needs to use an amount of disk space equal to the memory size). First, this assumes that you are actually going to use hibernate to disk (it's clearly very handy for laptops, but probably irrelevant for servers which are rarely turned off). Second it assumes that the hibernate to disk feature does actually use the swap partition for this purpose: I believe some systems use a dedicated hibernate partition, so then you wouldn't need to allocate extra swap space. So in some cases you could reduce the swap partition to 1 times memory size. It's hardly worth arguing about anyway, the difference in disk usage is likely to be 1% of the total, so probably most people won't even consider the options and just go with the default! Cheers -- Ian Disclaimer This communication is confidential and may contain privileged information intended solely for the named addressee(s). It may not be used or disclosed except for the purpose for which it has been sent. If you are not the intended recipient you must not review, use, disclose, copy, distribute or take any action in reliance upon it. If you have received this communication in error, please notify Astex Therapeutics Ltd by emailing i.tic...@astex-therapeutics.com and destroy all copies of the message and any attached documents. Astex Therapeutics Ltd monitors, controls and protects all its messaging traffic in compliance with its corporate email policy. The Company accepts no liability or responsibility for any onward transmission or use of emails and attachments having left the Astex Therapeutics domain. Unless expressly stated, opinions in this message are those of the individual sender and not of Astex Therapeutics Ltd. The recipient should check this email and any attachments for the presence of computer viruses. Astex Therapeutics Ltd accepts no liability for damage caused by any virus transmitted by this email. E-mail is susceptible to data corruption, interception, unauthorized amendment, and tampering, Astex Therapeutics Ltd only send and receive e-mails on the basis that the Company is not liable for any such alteration or any consequences thereof. Astex Therapeutics Ltd., Registered in England at 436 Cambridge Science Park, Cambridge CB4 0QA under number 3751674
[ccp4bb] phaser troubles
Hi This is probably an obvious one but I'm having trouble getting Phaser to run. It simply says the status is starting and will not give me more information in the log file. I suspect the problem has to do with the F and Sigma F values but I am not sure what to assign them as? Any help would be appreciated Sylvia htmlpfont face = verdana size = 0.8 color = navyThis communication is intended for the addressee only. It is confidential. If you have received this communication in error, please notify us immediately and destroy the original message. You may not copy or disseminate this communication without the permission of the University. Only authorized signatories are competent to enter into agreements on behalf of the University and recipients are thus advised that the content of this message may not be legally binding on the University and may contain the personal views and opinions of the author, which are not necessarily the views and opinions of The University of the Witwatersrand, Johannesburg. All agreements between the University and outsiders are subject to South African Law unless the University agrees in writing to the contrary./font/p/html image001.jpg
Re: [ccp4bb] phaser troubles
Hi, is the problem related to one of the problems listed below? (taken from the CCP4i troubleshooting page http://www.ccp4.ac.uk/ccp4i/trouble_shooting.html ): If I recall correctly, once I saw similar behaviour when my $CCP4I_TCLTK/tclsh was pointing to an incorrect directory. I hope that helps, Mark Jobs run but are reported as STARTING in the Job Window = When CCP4i runs a job it starts a separate process and this process reports back to the main CCP4i on progress - returning the status RUNNING, REMOTE,FINISHED or FAILED. However sometimes after jobs are reported as STARTING, the status never gets updated (even though the job runs or fails). There are several known problems which cause this to happen: On Windows systems, this may be a symptom of having spaces in directory or file names. Having spaces causes a variety of problems, and is best avoided. In older versions of the gui (pre CCP4 4.1) the communication between the two processes used the Tcl/Tk command send which didn't not work in Tcl/Tk built with the default options. As of CCP4 4.1 send is no longer used so this problem shouldn't arise. Check your version of CCP4 and upgrade, if necessary. When running jobs, CCP4i needs to be able to find the tclsh command in the directory pointed to by the CCP4I_TCLTK environment variable. For Unix/Linux systems this is set when you source the ccp4.setup file. If tclsh is not present then jobs may start but never actually run. Check that $CCP4I_TCLTK/tclsh exists, and edit your ccp4.setup file to point to the correct directory if it doesn't. This problem will also be observed if localhost is not defined in the system /etc/hosts. In this case a number of other symptoms will also be observed, for example 1) a warning message about running scripts being unable to connect to the server port; 2) neither the Run and view com file nor the Kill job options work. The fix is to make sure that localhost is defined in the /etc/hosts file. 2009/8/24 Sylvia Fanucchi sylvia.fanuc...@wits.ac.za Hi This is probably an obvious one but I’m having trouble getting Phaser to run. It simply says the status is “starting” and will not give me more information in the log file. I suspect the problem has to do with the F and Sigma F values but I am not sure what to assign them as? Any help would be appreciated Sylvia This communication is intended for the addressee only. It is confidential. If you have received this communication in error, please notify us immediately and destroy the original message. You may not copy or disseminate this communication without the permission of the University. Only authorized signatories are competent to enter into agreements on behalf of the University and recipients are thus advised that the content of this message may not be legally binding on the University and may contain the personal views and opinions of the author, which are not necessarily the views and opinions of The University of the Witwatersrand, Johannesburg. All agreements between the University and outsiders are subject to South African Law unless the University agrees in writing to the contrary. -- Mark Brooks, IBBMC, UMR8619 - Bâtiment 430, Université de Paris-Sud, 91405 Orsay, France. Tel: (33) 169157968 Fax: (33) 169853715 Skype: markabrooks
Re: [ccp4bb] Linux flavour and hard disks
Hi Claudia, There is an option that has not been mentioned yet and has a very obvious advantage: CentOS. I am mentioning this because it is identical in functionality to Red Hat and therefore it will take the least of your time to move to a new system. We like CentOS because it is derived from Red Hat, no surprises, no unexpected bugs. And unlike Red Hat, it is completely free and you don't need to talk to the Red Hat people (which I found to be completely impossible). As far as configuration goes, this is what we do: - Each workstation has a small (100GB or less) drive for the OS. Nothing else. - We have a network-attached disk farm that holds X-ray images on one share, programs on another, and personal data on a third. The advantage is that you need to maintain everything only in one place and it does not matter where you are working - the data and programs are transparently available to all workstations. - We have two storage devices, one primary, one backup. All data get backed up hourly (X-ray images) or daily (user data) or weekly (Programs). We currently have 2TB of space and probably will go to 4TB soon. You do need to make a careful analysis of what your needs are. We determined at the time that network traffic is never a limiting factor. Disk access can be somewhat of a limit, say when you have multiple people processing raw X-ray images at the same time, but that never happens in our group. So in the end we found that our limiting factor was C PU power and this is where we focused our attention to make an efficient system. There are slight advantages to each flavor of Linux, but my personal argument (as care taker) is that it should take as little time as possible to maintain your setup (because maintaining Linux systems is a necessity but not really what we wish to do). It should also be very reliable - it should always work and you want to make a careful plan to deal with disaster recovery - disks fail sooner or later and yes, I have had numerous users who accidentally did 'rm *' when they did not mean to do that... That, of course, works equally well in all Linux flavors. Mark -Original Message- From: Claudia Scotti claudiasco...@hotmail.com To: CCP4BB@JISCMAIL.AC.UK Sent: Mon, Aug 24, 2009 7:30 am Subject: [ccp4bb] Linux flavour and hard disks Dear List, I'm planning to migrate soon from Red Hat Linux 7.0 on an HP xw6000 workstation with dual Xeon processor. Please, any suggestion for the best Linux flavour to get the most out of today's crystallographic software? I've seen that both Ubuntu and Fedora are quite common. Also I'm in doubt about the following: will it be safer to use two mirror hard disks (as I'm doing now) or to use one HD for the software and one for the data? And, finally, please, what HD size is today most reasonable (big, but still fast enough)? Thanks a lot, Claudia=0 A Claudia Scotti Dipartimento di Medicina Sperimentale Sezione di Patologia Generale Universita' di Pavia Piazza Botta, 10 27100 Pavia Italia Tel. 0039 0382 986335/8/1 Facs 0039 0382 303673 check out the rest of the Windows Live™. More than mail–Windows Live™ goes way beyond your inbox. More than messages=
[ccp4bb] shape complementarity
Dear All, Could someone please suggest me program(s) to get the shape complementarity index for a protein complex structure of mine? Many thanks -- Amit Sharma, Ph.D. Research Associate, Department of Biology, University of York, YO10 5DD UK
Re: [ccp4bb] shape complementarity
amit sharma wrote: Dear All, Could someone please suggest me program(s) to get the shape complementarity index for a protein complex structure of mine? Many thanks sc, by Michael Lawrence: http://www.ccp4.ac.uk/dist/html/sc.html (included in ccp4 distro)
Re: [ccp4bb] phaser troubles
Hi, Sylvia: Did you also install Phenix in your machine? Phenix contains another version of phaser and will alias phaser to the phaser in Phenix. Try to type which phaser. If your command is not aliased to the phaser in your ccp4 version. Just use runview com file in the ccp4 and type in the phaser with the ccp4 directory (for example, /your directory/ccp4-6.1.1/ccp4-6.1.1/ccp4i/bin/phaser/). Meng-Chiao Joseph Ho Postdoc Dept. of Biochemistry Albert Einstein College of Medicine On Mon, 2009-08-24 at 18:03 +0200, Sylvia Fanucchi wrote: Hi This is probably an obvious one but I’m having trouble getting Phaser to run. It simply says the status is “starting” and will not give me more information in the log file. I suspect the problem has to do with the F and Sigma F values but I am not sure what to assign them as? Any help would be appreciated Sylvia This communication is intended for the addressee only. It is confidential. If you have received this communication in error, please notify us immediately and destroy the original message. You may not copy or disseminate this communication without the permission of the University. Only authorized signatories are competent to enter into agreements on behalf of the University and recipients are thus advised that the content of this message may not be legally binding on the University and may contain the personal views and opinions of the author, which are not necessarily the views and opinions of The University of the Witwatersrand, Johannesburg. All agreements between the University and outsiders are subject to South African Law unless the University agrees in writing to the contrary.
Re: [ccp4bb] phaser troubles
On Mon, Aug 24, 2009 at 12:30 PM, Joseph Ho j...@medusa.bioc.aecom.yu.eduwrote: Did you also install Phenix in your machine? Phenix contains another version of phaser and will alias phaser to the phaser in Phenix. No, it will be called phenix.phaser. The Phenix environment scripts ($PHENIX/phenix_env*) do not alias anything; they only add the directory containing the command-line launchers to PATH. If used properly, you shouldn't see any conflicts with CCP4 or anything else.
[ccp4bb] FPLC for sale
Dear All: Please see attached is an ad for our AKTAprime plus FPLC system which is only 2 years old, used a dozen times and in excellent condition. Thanks, Natalie Roy FPLC for sale.doc Description: MS-Word document
[ccp4bb] Sketcher and Refmac monomer library construction
CCP4bbers, I am trying to do a restrained refinement in refmac using a rhenium tricarbonyl (RTC) fragment similar to the one in PDB 1I53. My understanding of how to proceed is to take the PDB description of the file, read it into sketcher, and then run libcheck to create the various cif and lib files in my local directory. Assuming I can get this to work (see below), then refmac should recognize the monomer files in the local directory from the residue name and use the proper restraints? Or do I need to explicitly read in the lib file(s) (e.g., libcheck_RTC.cif) in the LibIn field in CCP4i? I can read in the RTC PDB file below into sketcher and change the CO bonds to triple (they come up double on read-in), but then running libcheck dutifully makes the carbons sp2 and adds riding hydrogens. Agh. Running libcheck at the command line does the same thing with or without the HFLAG set. I would be grateful for any assistance/suggestions. I thought this would be an easy task... :) I must be missing something obvious. BTW, the RTC monomer in the HIC-UP server is incorrect: it is meridional instead of (the correct) facial geometry. THe PDB file below is correct. ATOM 1976 RE RTC B 905 39.199 36.612 61.900 1.00 20.26 RE ATOM 1977 O1 RTC B 905 41.664 36.504 60.063 1.00 20.75 O ATOM 1978 O2 RTC B 905 40.911 37.753 64.220 1.00 20.54 O ATOM 1979 O3 RTC B 905 39.925 33.794 62.879 1.00 20.49 O ATOM 1980 C1 RTC B 905 40.757 36.541 60.750 1.00 20.06 C ATOM 1981 C2 RTC B 905 40.269 37.327 63.349 1.00 20.00 C ATOM 1982 C3 RTC B 905 39.667 34.827 62.518 1.00 20.04 C Cheers, -- Roger S. Rowlett Professor Department of Chemistry Colgate University 13 Oak Drive Hamilton, NY 13346 tel: (315)-228-7245 ofc: (315)-228-7395 fax: (315)-228-7935 email: rrowl...@mail.colgate.edu
[ccp4bb] How to work around strange naming XYZIN convention?
Not sure this is the right place to ask, but... I'm trying to chain some ccp4 programs together via a script. It creates temporary files with arbitrary names based on the process id. The problem is that when I try to pass one of these files to the next program in the chain, as in: tlsanl XYZIN $tempfile EOF ... EOF The ccp4 libraries seem to expand this to be an input file named $tempfile.brk Is there any way to make the programs _not_ append a useless extension .brk in a scripted environment? -- Ethan A Merritt Biomolecular Structure Center University of Washington, Seattle 98195-7742
Re: [ccp4bb] Linux flavour and hard disks
On Aug 24, 2009, at 8:32 AM, William G. Scott wrote: On Aug 24, 2009, at 7:49 AM, ar...@xtals.org wrote: my two cents: don't get involved in nerdy discussions Here's a useful summary: So it's freebsd then?
[ccp4bb] FPLC for sale *PRICE*
Hi everyone, Thank you for all the replies to the ad I posted about the FPLC for sale, we are asking $11,000 CAD for the system and all of its components, but we are willing to negotiate price. The UV lamp is also 2 years old but only been used about a dozen times. Shipping will be discussed with those who are interested because it will be dependant on the location of the buyer whether or not it's included in the price. Thanks! Nat
