[ccp4bb] Position available: Senior Technical Officer (Macromolecular Crystallisation) - Monash University, Australia

[Jennifer Huynh]

Dear All

We are looking to recruit a Senior Technical Officer for the Monash
Macromolecular Crystallisation Facility (MMCF) at Monash University,
Australia.

The MMCF is a highly specialised Core Facility of Monash Structural Biology
Platform. It delivers access to a fully automated platform for the
high-throughput crystallisation of biological macromolecules.

As a senior technical officer, you will  be responsible for overseeing and
delivering high quality technical services to support the operations of the
MMCF. You will also perform a range of significant and complex technical
activities that play a critical role in supporting the delivery of the
outcomes of the facility. This includes overseeing and undertaking testing,
equipment maintenance, developing operating procedures, data analysis,
while ensuring a compliant and safe operating environment.

You will be a subject matter expert and provide strategic and operational
advice to clients and deliver efficient technical services with a focus on
operational excellence.

If you believe you can fulfil these requirements, you are strongly
encouraged to apply.

See:
http://careersmanager.pageuppeople.com/513/cw/en/job/561493/senior-technical-officer-macromolecular-crystallisation?=11299

Enquiries or any questions: jennifer.hu...@monash.edu

Closing date: May 28, 2017.

Kind regards
Jennifer

-- 
*JENNIFER HUYNH*
*Senior Administration Coordinator *
*Office of Prof. Jamie Rossjohn, FAA FLSW FMedSci*
*ARC Australian Laureate Fellow*

*Department of Biochemistry & Molecular Biology/ *
*School of Biomedical Sciences*
Monash University
Ground floor, Building 76, Clayton Campus
19 Innovation Walk
Clayton VIC 3800
Australia

T: +61 3 990 29252
F: +61 3 990 55645
E: jennifer.hu...@monash.edu 
W: http://research.med.monash.edu.au/rossjohn/

Twitter: @RossjohnLab
Facebook: www.facebook.com/pages/Rossjohn-Lab/1460515330888135
*Please note: I work part-time Tuesdays & Wednesdays in the office*

Re: [ccp4bb] Different loop conformations

[Eleanor Dodson]

well - Q1. I think you can observe it..
And there is no format problems in modelling several residues as
conformation A and B
Eleanor

On 16 May 2017 at 19:33, Sam Tang  wrote:

> Dear all
>
> Sorry for the slightly off-topic thread.
>
> We are lucky enough to have recently solved a protein-ligand structure in
> P1 space to 2.4 A by molecular replacement using the apo-protein as model.
> The protein is known to have a flexible loop region of about 20 amino acid
> long. In the apo-protein (model), density for > half of this loop are not
> seen.
>
> It seems interesting to us that, in the complex structure, although still
> incomplete, there seems to be loop densities at two distinct conformations
> (named up and down, which point to opposite directions almost 120 degree)
> -- when we fit the loop to the up conformation, difference map shows green
> blots at the down conformation, and vice versa.
>
> What we feel puzzled are:
> (1) Is it possible, at this resolution, for the two conformations be
> observed? Or is there any similar case we can make reference to?
>
> (2) If the densities below to two conformations of the loop, how should we
> model it?  Add alternate conformation function in Coot doesn't seem
> suitable. Or should we make up (and eventually deposit) two models
> showcasing the two conformations?
>
> Many thanks in advance for your attention!
>
> Regards
>
> Sam
>
>
>

[ccp4bb] Different loop conformations

[Sam Tang]

Dear all

Sorry for the slightly off-topic thread.

We are lucky enough to have recently solved a protein-ligand structure in
P1 space to 2.4 A by molecular replacement using the apo-protein as model.
The protein is known to have a flexible loop region of about 20 amino acid
long. In the apo-protein (model), density for > half of this loop are not
seen.

It seems interesting to us that, in the complex structure, although still
incomplete, there seems to be loop densities at two distinct conformations
(named up and down, which point to opposite directions almost 120 degree)
-- when we fit the loop to the up conformation, difference map shows green
blots at the down conformation, and vice versa.

What we feel puzzled are:
(1) Is it possible, at this resolution, for the two conformations be
observed? Or is there any similar case we can make reference to?

(2) If the densities below to two conformations of the loop, how should we
model it?  Add alternate conformation function in Coot doesn't seem
suitable. Or should we make up (and eventually deposit) two models
showcasing the two conformations?

Many thanks in advance for your attention!

Regards

Sam

[ccp4bb] Cryo-EM School2017 in Pavia: Deadline Extended, but few places remaining

[Federico Forneris]

Dear all,

Few places are still available for the AIC School "Bridging the gap between
Cryo-EM and Crystallography". http://www.cristallografia.org/aicschool2017 

We have decided to extend the bursary registration deadline until May 30th,
2017. We wish to remind applicants that have received official sponsorship
from the International Union for Crystallography (IUCr) which allows us to
offer travel bursaries and awards to outstanding participants based on their
CV and motivation letters.

 

This school will offer the opportunity to young researchers in biochemistry
or structural biology at the PhD/postdoctoral level to approach the
fundamental questions arising from the cryo-EM experiment setup. Central
topics will include evaluation sample suitability for analysis and its
preparation, choice of appropriate instrumentation for single particle,
tomography or electron diffraction approaches, data processing and
interpretation of results. A showcase of recent results will contour the
tutorial sessions.

 

We are confident that this event will be an excellent opportunity for many
scientists who want to approach and learn the basics of cryo-electron
microscopy. 

 

For information, program and registration details please visit
http://www.cristallografia.org/aicschool2017. More specific enquiries can be
discussed via e-mail to my address or to aicsch...@cristallografia.org 

 

We look forward to hosting you in Pavia and discuss about Cryo-EM in
September.

On behalf of all school organizers,

Very best wishes,

F.

 

Federico Forneris, PhD


The Armenise-Harvard Laboratory of Structural Biology

Dept. Biology and Biotechnology
University of Pavia

Via Ferrata, 9

I-27100 Pavia - ITALY
  http://fornerislab.unipv.it

 

 

 

 

[ccp4bb] PhD Positions at the University of Dundee

[Paul Fyfe (Staff)]

The University of Dundee has several PhD positions open for immediate 
applications. Project areas cover informatics, structural biology, medicinal 
and biological chemistry and molecular biology.

For details go to: 
https://www.findaphd.com/search/PhDDetails.aspx?CAID=3490_content=buff

Closing date for applications is Friday 2nd of June 2017

The University of Dundee is a registered Scottish Charity, No: SC015096

[ccp4bb] Postdoctoral Position available at The Institute of Cancer Research, London UK

[Rob Van Montfort]

The Institute of Cancer Research, London, is one of the world’s most 
influential cancer research institutes, with an outstanding record of 
achievement dating back more than 100 years. We provided the first convincing 
evidence that DNA damage is the basic cause of cancer, laying the foundation 
for the now universally accepted idea that cancer is a genetic disease. Today, 
The Institute of Cancer Research (ICR) leads the world at isolating 
cancer-related genes and discovering new targeted drugs for personalised cancer 
treatment. Under the leadership of our Chief Executive, Professor Paul Workman 
FMedSci, the ICR is ranked as the UK’s leading academic research centre. 
Together with our partner The Royal Marsden, we are rated in the top four 
cancer centres globally.The ICR is committed to attracting, developing and 
retaining the best minds in the world to join us in our mission – to make the 
discoveries that defeat cancer.
The Cancer Research UK Cancer Therapeutics Unit (CTU), within the Division of 
Cancer Therapeutics, is a multidisciplinary 'bench to bedside' centre, 
comprising around 160 staff dedicated to the discovery and development of novel 
therapeutics for the treatment of cancer. The Cancer Therapeutics Unit’s 
exciting goal is to discover high quality small molecule drug candidates and to 
progress these to clinical trial. All the scientific disciplines are in place 
to make this possible, including medicinal chemistry, biology, drug metabolism 
and clinical specialists who focus on new molecular targets emerging from human 
genome and ground breaking cell biology research.
A postdoctoral position is now available in the Hit Discovery and Structural 
Design Team within the CTU (Ref. 1646606). The team combines assay development 
and small-molecule high-throughput screening with fragment-based hit discovery, 
biophysical assays and X-ray crystallography. These methodologies are 
underpinned by state-of-the art protein expression, purification and protein 
characterisation capabilities allowing the generation of large quantities of 
high quality recombinant proteins. The successful candidate will work on an 
exciting early stage target in CTU’s drug discovery portfolio. The postdoctoral 
training fellow will establish the successful expression, purification and 
characterisation of the protein target, using contemporary expression systems, 
including insect and mammalian cells, and purification methods available within 
the team. They will establish the in-house crystallisation and structure 
determination and subsequently carry out the structural determination of 
protein-inhibitor complexes. In addition, the post-holder is expected to 
initiate the development of biochemical and biophysical assays to characterise 
the protein target and to establish compound screening assays. The successful 
candidate will be an integral member of a multidisciplinary project team and 
will interact closely with the biologists, computational chemists, medicinal 
chemists and structural biologists and will therefore be expected to work 
across the two sites in Chelsea, London and Sutton, Surrey.
Applicants must have a PhD in a biological or physical science, and experience 
in macromolecular crystallography (to include protein biochemistry, protein 
crystallisation, & protein crystallography). Experience in insect 
cell/mammalian cell expression, assay development, and/or biophysics will be 
highly advantageous. The starting salary for this position will be up to 
£35,538p.a. inclusive (based on previous experience) and the post is offered on 
a fixed term contract of 1 year.
Further information about these positions can be found in the full job 
description at www.icr.ac.uk. Informal enquiries to 
rob.vanmontf...@icr.ac.uk - but applications 
must be submitted on-line (www.icr.ac.uk). Closing date 
is June 11th.

Dr. Rob van Montfort
Team Leader Hit Discovery and Structural Design
Joint Interim Head of Division of Structural Biology
Divisions of Cancer Therapeutics and Structural Biology
The Institute of Cancer Research
15 Cotswold Road
Sutton SM2 5NG
UK

Tel:
+44-(0)20-8722-4364 (Sutton)
+44-(0)20-7153-5142 (Chelsea)
Email: rob.vanmontf...@icr.ac.uk







The Institute of Cancer Research: Royal Cancer Hospital, a charitable Company 
Limited by Guarantee, Registered in England under Company No. 534147 with its 
Registered Office at 123 Old Brompton Road, London SW7 3RP.

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[ccp4bb] Senior Support Scientist position at Diamond Light Source beamline B24

[Maria Harkiolaki]

Dear all,


We are seeking to recruit a Senior Support Scientist to join the B24 cryo X-ray 
tomography team.


The post holder is expected to help with the operation and development of the 
beamline through contributing to user support, participation in the in-house 
research and development programme and general house-keeping tasks (instrument 
support,  maintaining webpages and up keep of beamline documentation).

The post holder is also expected to aid in the design, construction, 
installation and commissioning of beamline modules.


Candidates should ideally have an excellent understanding of current biomedical 
research requirements, experience in cellular microscopy (preferably 
cryo-microscopy of biological samples) and be familiar with synchrotron 
radiation beamlines. Experience in fluorescence microscopy as well as life 
science research (tissue culture, sample handling and processing) and logistics 
is desirable. Training will be provided where necessary.


You can find further information at 
http://www.diamond.ac.uk/Careers/Vacancies/All/050_17_CH.html

This is a 2 year fixed full time post and the application deadline is the 11th 
of June, 2017.

Kind regards,

Maria



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