Re: [ccp4bb] Interesting pattern on a crystallization drop

[Artem Evdokimov]

Neat!

Looks like multiple adjacent bubbles that were initially touching but
eventually shrunk down to the central cores - the connectors are protein
filaments (skin on the bubbles) left over from when bubbles had contact
points.

Artem

On Wed, Mar 27, 2019, 19:39 Marshall, Bevan (Manufacturing, Parkville)
 wrote:

> Looked up the condition on C6 (https://c6.csiro.au/C6.asp) and that
> condition is found in both Index and JCSG screens as well as Classics II.
>
>
>
>
>
> *Bevan Marshall*
> Staff Scientist | Collaborative Crystallisation Centre
> *Manufacturing*
> CSIRO
>
> E bevan.marsh...@csiro.aut +61 3 9662 7492
> 343-351 Royal Parade, Parkville, VIC 3052
> www.csiro.au | https://c3.csiro.au
> CSIRO acknowledges the Traditional Owners of the lands that we live and
> work on across Australia and pays its respect to Elders past and present.
>
> *PLEASE NOTE*
> The information contained in this email may be confidential or privileged.
> Any unauthorised use or disclosure is prohibited. If you have received this
> email in error, please delete it immediately and notify the sender by
> return email. Thank you. To the extent permitted by law, CSIRO does not
> represent, warrant and/or guarantee that the integrity of this
> communication has been maintained or that the communication is free of
> errors, virus, interception or interference.
>
> *Please consider the environment before printing this email.*
>
>
>
> *From:* CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] *On Behalf Of 
> *LEGRAND
> Pierre
> *Sent:* Thursday, 28 March 2019 9:13 AM
> *To:* CCP4BB@JISCMAIL.AC.UK
> *Subject:* Re: [ccp4bb] Interesting pattern on a crystallization drop
>
>
>
> Dear Beatriz,
>
>
>
> Nice drops :-))
>
> Could it be that there is a reaction going on in these drops ?
>
> The conditions are quite "exotic" with possibilities of coordination or
> oxydoreduction (Co2+/Co3+) or polymerization...
>
> Do you have reductants with the protein buffer ?
>
> Is the protein an enzyme or a metalloprotein ?
>
> Just some ideas.
>
>
>
> Best wishes,
>
> Pierre
>
>
> --
>
> *De :* CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] de la part de Beatriz
> Gomes Guimaraes [beatriz.guimar...@fiocruz.br]
> *Envoyé :* mercredi 27 mars 2019 19:44
> *À :* CCP4BB@JISCMAIL.AC.UK
> *Objet :* [ccp4bb] Interesting pattern on a crystallization drop
>
> Dear all,
>
>
>
> I would like to share with you a surprising pattern I found when examining
> some crystallization plates (attached figures).
>
>
>
> It is less obvious looking the photos, but apparently the "lines" are
> formed by precipitated protein and there are some "bubbles" with small
> drops inside. I wish they were microcrystals but I do not think this is
> the case.
>
> I was suprised by the symmetry !
>
>
>
> And it is not completely random because for the same condition the
> difference between the two drops are : protein alone ("hexagon") and
> protein + ligand ("rhombus")
>
>
>
> crystallization condition is:
>
> 0.01 M Cobalt(II) chloride hexahydrate
>
> 0.1 M Tris pH 8.5
>
> 20% w/v Polyvinylpyrrolidone K 15
>
>
>
> Have you seen anything similar before?
>
>
>
> Thank you for your comments!
>
> Beatriz
>
>
>
>
>
> --
> Beatriz Guimarães
> Laboratory of Structural Biology and Protein Engineering
> Instituto Carlos Chagas - ICC / FIOCRUZ Paraná
> Rua Prof. Algacyr Munhoz Mader, 3775   Bloco C
> CIC 81350-010
> Curitiba - PR, Brasil
> Tel.:+55(41)3316-3225/2104-3438
>
>
> --
>
> To unsubscribe from the CCP4BB list, click the following link:
> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1
>
>
> --
>
> To unsubscribe from the CCP4BB list, click the following link:
> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1
>
> --
>
> To unsubscribe from the CCP4BB list, click the following link:
> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1
>



To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1

Re: [ccp4bb] Interesting pattern on a crystallization drop

[Marshall, Bevan (Manufacturing, Parkville)]

Looked up the condition on C6 (https://c6.csiro.au/C6.asp) and that condition 
is found in both Index and JCSG screens as well as Classics II.


Bevan Marshall
Staff Scientist | Collaborative Crystallisation Centre
Manufacturing
CSIRO
E bevan.marsh...@csiro.auT +61 3 9662 7492
343-351 Royal Parade, Parkville, VIC 3052
www.csiro.au | https://c3.csiro.au
CSIRO acknowledges the Traditional Owners of the lands that we live and work on 
across Australia and pays its respect to Elders past and present.
PLEASE NOTE
The information contained in this email may be confidential or privileged. Any 
unauthorised use or disclosure is prohibited. If you have received this email 
in error, please delete it immediately and notify the sender by return email. 
Thank you. To the extent permitted by law, CSIRO does not represent, warrant 
and/or guarantee that the integrity of this communication has been maintained 
or that the communication is free of errors, virus, interception or 
interference.
Please consider the environment before printing this email.

From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of LEGRAND 
Pierre
Sent: Thursday, 28 March 2019 9:13 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Interesting pattern on a crystallization drop

Dear Beatriz,

Nice drops :-))
Could it be that there is a reaction going on in these drops ?
The conditions are quite "exotic" with possibilities of coordination or 
oxydoreduction (Co2+/Co3+) or polymerization...
Do you have reductants with the protein buffer ?
Is the protein an enzyme or a metalloprotein ?
Just some ideas.

Best wishes,
Pierre


De : CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] de la part de Beatriz Gomes 
Guimaraes [beatriz.guimar...@fiocruz.br]
Envoyé : mercredi 27 mars 2019 19:44
À : CCP4BB@JISCMAIL.AC.UK
Objet : [ccp4bb] Interesting pattern on a crystallization drop

Dear all,



I would like to share with you a surprising pattern I found when examining some 
crystallization plates (attached figures).



It is less obvious looking the photos, but apparently the "lines" are formed by 
precipitated protein and there are some "bubbles" with small drops inside. I 
wish they were microcrystals but I do not think this is the case.

I was suprised by the symmetry !



And it is not completely random because for the same condition the difference 
between the two drops are : protein alone ("hexagon") and protein + ligand 
("rhombus")


crystallization condition is:

0.01 M Cobalt(II) chloride hexahydrate

0.1 M Tris pH 8.5

20% w/v Polyvinylpyrrolidone K 15



Have you seen anything similar before?



Thank you for your comments!

Beatriz




--
Beatriz Guimarães
Laboratory of Structural Biology and Protein Engineering
Instituto Carlos Chagas - ICC / FIOCRUZ Paraná
Rua Prof. Algacyr Munhoz Mader, 3775   Bloco C
CIC 81350-010
Curitiba - PR, Brasil
Tel.:+55(41)3316-3225/2104-3438



To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1



To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1



To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1

Re: [ccp4bb] Interesting pattern on a crystallization drop

[LEGRAND Pierre]

Dear Beatriz,

Nice drops :-))
Could it be that there is a reaction going on in these drops ?
The conditions are quite "exotic" with possibilities of coordination or 
oxydoreduction (Co2+/Co3+) or polymerization...
Do you have reductants with the protein buffer ?
Is the protein an enzyme or a metalloprotein ?
Just some ideas.

Best wishes,
Pierre


De : CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] de la part de Beatriz Gomes 
Guimaraes [beatriz.guimar...@fiocruz.br]
Envoyé : mercredi 27 mars 2019 19:44
À : CCP4BB@JISCMAIL.AC.UK
Objet : [ccp4bb] Interesting pattern on a crystallization drop


Dear all,


I would like to share with you a surprising pattern I found when examining some 
crystallization plates (attached figures).


It is less obvious looking the photos, but apparently the "lines" are formed by 
precipitated protein and there are some "bubbles" with small drops inside. I 
wish they were microcrystals but I do not think this is the case.

I was suprised by the symmetry !


And it is not completely random because for the same condition the difference 
between the two drops are : protein alone ("hexagon") and protein + ligand 
("rhombus")


crystallization condition is:

0.01 M Cobalt(II) chloride hexahydrate

0.1 M Tris pH 8.5

20% w/v Polyvinylpyrrolidone K 15


Have you seen anything similar before?


Thank you for your comments!

Beatriz



--
Beatriz Guimarães
Laboratory of Structural Biology and Protein Engineering
Instituto Carlos Chagas - ICC / FIOCRUZ Paraná
Rua Prof. Algacyr Munhoz Mader, 3775   Bloco C
CIC 81350-010
Curitiba - PR, Brasil
Tel.:+55(41)3316-3225/2104-3438



To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1



To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1

Re: [ccp4bb] Interesting pattern on a crystallization drop

[Beatriz Gomes Guimaraes]

Dear all,


some more details:

drops were set up by a robot (Mosquito), 200 nL prot+ 200 nL reservoir on a 
96-Well sitting drop iQ Plate (TTPlabtech). They are not really bubbles. I have 
the impression that the lines are all around creating these "empty rounds" 
(maybe "exploded bubbles"?)  with the small drop inside.


Beatriz


--
Beatriz Guimarães
Laboratory of Structural Biology and Protein Engineering
Instituto Carlos Chagas - ICC / FIOCRUZ Paraná
Rua Prof. Algacyr Munhoz Mader, 3775   Bloco C
CIC 81350-010
Curitiba - PR, Brasil
Tel.:+55(41)3316-3225/2104-3438

De: Ailong Ke 
Enviado: quarta-feira, 27 de março de 2019 17:06:01
Para: Beatriz Gomes Guimaraes
Cc: CCP4BB@JISCMAIL.AC.UK
Assunto: Re: [ccp4bb] Interesting pattern on a crystallization drop

Hi Beatriz,

Were the drops set up by hand or by a robot? If the latter, what kind of robot? 
are they hanging drops or sitting drops, and do the bubbles form on the glass 
surface of at the bottom of your drop?

AK

On Mar 27, 2019, at 2:44 PM, Beatriz Gomes Guimaraes 
mailto:beatriz.guimar...@fiocruz.br>> wrote:

Dear all,

I would like to share with you a surprising pattern I found when examining some 
crystallization plates (attached figures).

It is less obvious looking the photos, but apparently the "lines" are formed by 
precipitated protein and there are some "bubbles" with small drops inside. I 
wish they were microcrystals but I do not think this is the case.
I was suprised by the symmetry !

And it is not completely random because for the same condition the difference 
between the two drops are : protein alone ("hexagon") and protein + ligand 
("rhombus")

crystallization condition is:
0.01 M Cobalt(II) chloride hexahydrate
0.1 M Tris pH 8.5
20% w/v Polyvinylpyrrolidone K 15

Have you seen anything similar before?

Thank you for your comments!
Beatriz


--
Beatriz Guimarães
Laboratory of Structural Biology and Protein Engineering
Instituto Carlos Chagas - ICC / FIOCRUZ Paraná
Rua Prof. Algacyr Munhoz Mader, 3775   Bloco C
CIC 81350-010
Curitiba - PR, Brasil
Tel.:+55(41)3316-3225/2104-3438


To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1





To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1

Re: [ccp4bb] Interesting pattern on a crystallization drop

[Ailong Ke]

Hi Beatriz,

Were the drops set up by hand or by a robot? If the latter, what kind of robot? 
are they hanging drops or sitting drops, and do the bubbles form on the glass 
surface of at the bottom of your drop?

AK

On Mar 27, 2019, at 2:44 PM, Beatriz Gomes Guimaraes 
mailto:beatriz.guimar...@fiocruz.br>> wrote:

Dear all,

I would like to share with you a surprising pattern I found when examining some 
crystallization plates (attached figures).

It is less obvious looking the photos, but apparently the "lines" are formed by 
precipitated protein and there are some "bubbles" with small drops inside. I 
wish they were microcrystals but I do not think this is the case.
I was suprised by the symmetry !

And it is not completely random because for the same condition the difference 
between the two drops are : protein alone ("hexagon") and protein + ligand 
("rhombus")

crystallization condition is:
0.01 M Cobalt(II) chloride hexahydrate
0.1 M Tris pH 8.5
20% w/v Polyvinylpyrrolidone K 15

Have you seen anything similar before?

Thank you for your comments!
Beatriz


--
Beatriz Guimarães
Laboratory of Structural Biology and Protein Engineering
Instituto Carlos Chagas - ICC / FIOCRUZ Paraná
Rua Prof. Algacyr Munhoz Mader, 3775   Bloco C
CIC 81350-010
Curitiba - PR, Brasil
Tel.:+55(41)3316-3225/2104-3438


To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1





To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1

Re: [ccp4bb] Interesting pattern on a crystallization drop

[Keller, Jacob]

It's a crystal with a very large lattice.

JPK

+
Jacob Pearson Keller
Research Scientist / Looger Lab
HHMI Janelia Research Campus
19700 Helix Dr, Ashburn, VA 20147
Desk: (571)209-4000 x3159
Cell: (301)592-7004
+

The content of this email is confidential and intended for the recipient 
specified in message only. It is strictly forbidden to share any part of this 
message with any third party, without a written consent of the sender. If you 
received this message by mistake, please reply to this message and follow with 
its deletion, so that we can ensure such a mistake does not occur in the future.

From: CCP4 bulletin board  On Behalf Of Beatriz Gomes 
Guimaraes
Sent: Wednesday, March 27, 2019 2:44 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Interesting pattern on a crystallization drop


Dear all,



I would like to share with you a surprising pattern I found when examining some 
crystallization plates (attached figures).



It is less obvious looking the photos, but apparently the "lines" are formed by 
precipitated protein and there are some "bubbles" with small drops inside. I 
wish they were microcrystals but I do not think this is the case.

I was suprised by the symmetry !



And it is not completely random because for the same condition the difference 
between the two drops are : protein alone ("hexagon") and protein + ligand 
("rhombus")


crystallization condition is:

0.01 M Cobalt(II) chloride hexahydrate

0.1 M Tris pH 8.5

20% w/v Polyvinylpyrrolidone K 15



Have you seen anything similar before?



Thank you for your comments!

Beatriz




--
Beatriz Guimarães
Laboratory of Structural Biology and Protein Engineering
Instituto Carlos Chagas - ICC / FIOCRUZ Paraná
Rua Prof. Algacyr Munhoz Mader, 3775   Bloco C
CIC 81350-010
Curitiba - PR, Brasil
Tel.:+55(41)3316-3225/2104-3438



To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1



To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1

Re: [ccp4bb] Interesting pattern on a crystallization drop

[Newman, Janet (Manufacturing, Parkville)]

I'm guessing quite big drops on a somewhat hydrophobic surface? Also guessing 
that the protein foams quite a bit, and that there were 6 (or four) more or 
less equal size bubbles that took up almost the whole drop when it was set up. 
Bubbles tend to pack quite efficiently 
(https://physicsworld.com/a/getting-to-the-bottom-of-foamy-physics/), giving 
the base symmetry that stayed when the bubbles burst. Wondering if the lines 
are from the collapse of the thin protein/polymer skin covering the surface of 
the bubbles?


lovely and thought provoking images - thanks!


Janet

[https://physicsworld.com/wp-content/uploads/2013/05/bubbles.jpg]

Getting to the bottom of foamy physics – Physics 
World
physicsworld.com
Researchers in the US have created a new mathematical model to describe the 
complex evolution of foamy bubbles – something that has proved fiendishly 
difficult to model thanks to the hugely varying length and time scales 
involved. Their computed results closely match theoretical models as well as ...




Janet Newman
Principal Scientist / Director, Collaborative Crystallisation Centre (C3)
CSIRO Material Science and Engineering
343 Royal Parade
Parkville.  VIC. 3052
Australia
Tel +613 9662 7326
Email janet.new...@csiro.au



From: CCP4 bulletin board  on behalf of Beatriz Gomes 
Guimaraes 
Sent: Thursday, March 28, 2019 5:44 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Interesting pattern on a crystallization drop


Dear all,


I would like to share with you a surprising pattern I found when examining some 
crystallization plates (attached figures).


It is less obvious looking the photos, but apparently the "lines" are formed by 
precipitated protein and there are some "bubbles" with small drops inside. I 
wish they were microcrystals but I do not think this is the case.

I was suprised by the symmetry !


And it is not completely random because for the same condition the difference 
between the two drops are : protein alone ("hexagon") and protein + ligand 
("rhombus")


crystallization condition is:

0.01 M Cobalt(II) chloride hexahydrate

0.1 M Tris pH 8.5

20% w/v Polyvinylpyrrolidone K 15


Have you seen anything similar before?


Thank you for your comments!

Beatriz



--
Beatriz Guimarães
Laboratory of Structural Biology and Protein Engineering
Instituto Carlos Chagas - ICC / FIOCRUZ Paraná
Rua Prof. Algacyr Munhoz Mader, 3775   Bloco C
CIC 81350-010
Curitiba - PR, Brasil
Tel.:+55(41)3316-3225/2104-3438



To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1



To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1

Re: [ccp4bb] Interesting pattern on a crystallization drop

[Beatriz Gomes Guimaraes]

Hi Philippe

I do not know yet if it is reproducible. And protein function has nothing to do 
with polymerization or something ... it is a RNA interacting protein, small and 
usually monomeric.

I will try to find a better one for the April 1st... but it will be hard!

Best regards,

Beatriz


--
Beatriz Guimarães
Laboratory of Structural Biology and Protein Engineering
Instituto Carlos Chagas - ICC / FIOCRUZ Paraná
Rua Prof. Algacyr Munhoz Mader, 3775   Bloco C
CIC 81350-010
Curitiba - PR, Brasil
Tel.:+55(41)3316-3225/2104-3438



De: DUMAS Philippe (IGBMC) 
Enviado: quarta-feira, 27 de março de 2019 16:34
Para: Beatriz Gomes Guimaraes
Cc: CCP4BB@JISCMAIL.AC.UK
Assunto: Re: [ccp4bb] Interesting pattern on a crystallization drop


Le Mercredi 27 Mars 2019 19:44 CET, Beatriz Gomes Guimaraes 
 a écrit:

In four days from now, I (everyone) would have taken this as a Photoshop joke...
Quite amazing! Is it reproducible?
What is the protein? Tubulin ? ::))
Philippe Dumas


> Dear all,
>
>
> I would like to share with you a surprising pattern I found when examining 
> some crystallization plates (attached figures).
>
>
> It is less obvious looking the photos, but apparently the "lines" are formed 
> by precipitated protein and there are some "bubbles" with small drops inside. 
> I wish they were microcrystals but I do not think this is the case.
>
> I was suprised by the symmetry !
>
>
> And it is not completely random because for the same condition the difference 
> between the two drops are : protein alone ("hexagon") and protein + ligand 
> ("rhombus")
>
>
> crystallization condition is:
>
> 0.01 M Cobalt(II) chloride hexahydrate
>
> 0.1 M Tris pH 8.5
>
> 20% w/v Polyvinylpyrrolidone K 15
>
>
> Have you seen anything similar before?
>
>
> Thank you for your comments!
>
> Beatriz
>
>
>
> --
> Beatriz Guimarães
> Laboratory of Structural Biology and Protein Engineering
> Instituto Carlos Chagas - ICC / FIOCRUZ Paraná
> Rua Prof. Algacyr Munhoz Mader, 3775   Bloco C
> CIC 81350-010
> Curitiba - PR, Brasil
> Tel.:+55(41)3316-3225/2104-3438
>
> 
>
> To unsubscribe from the CCP4BB list, click the following link:
> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1









To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1

Re: [ccp4bb] Interesting pattern on a crystallization drop

[IGBMC]

Le Mercredi 27 Mars 2019 19:44 CET, Beatriz Gomes Guimaraes 
 a écrit:

In four days from now, I (everyone) would have taken this as a Photoshop joke...
Quite amazing! Is it reproducible?
What is the protein? Tubulin ? ::))
Philippe Dumas


> Dear all,
>
>
> I would like to share with you a surprising pattern I found when examining 
> some crystallization plates (attached figures).
>
>
> It is less obvious looking the photos, but apparently the "lines" are formed 
> by precipitated protein and there are some "bubbles" with small drops inside. 
> I wish they were microcrystals but I do not think this is the case.
>
> I was suprised by the symmetry !
>
>
> And it is not completely random because for the same condition the difference 
> between the two drops are : protein alone ("hexagon") and protein + ligand 
> ("rhombus")
>
>
> crystallization condition is:
>
> 0.01 M Cobalt(II) chloride hexahydrate
>
> 0.1 M Tris pH 8.5
>
> 20% w/v Polyvinylpyrrolidone K 15
>
>
> Have you seen anything similar before?
>
>
> Thank you for your comments!
>
> Beatriz
>
>
>
> --
> Beatriz Guimarães
> Laboratory of Structural Biology and Protein Engineering
> Instituto Carlos Chagas - ICC / FIOCRUZ Paraná
> Rua Prof. Algacyr Munhoz Mader, 3775   Bloco C
> CIC 81350-010
> Curitiba - PR, Brasil
> Tel.:+55(41)3316-3225/2104-3438
>
> 
>
> To unsubscribe from the CCP4BB list, click the following link:
> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1







To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1

[ccp4bb] CCP-EM Spring Symposium - registration closes this Friday (29th March)

[Tom Burnley - UKRI STFC]

Dear all,

Just a quick reminder that registration for the Spring Symposium will close 
this Friday (29th March).

Full details and talk titles can be found here:

http://www.cvent.com/d/xbqsqk

The Spring Symposium aims to provide a forum to highlight state of the art 
developments in computational cryo-EM and related themes as well as showcasing 
outstanding recent applications. We aim to promote an inclusive, friendly 
atmosphere welcoming both old and new to the community and there is the 
opportunity to present your work via a poster or a selected talk.

Topics include instrument technology, sample preparation, image processing, 
single particle reconstruction, tomography and model building.

The conference will be held 29th April - 1st May at the University of 
Nottingham, UK & the first day (29th April) will include the Biological 
Cryo-imaging User Meeting for eBIC & B24.

Confirmed speakers & talk titles include:

John Rubinstein (University of Toronto) - CryoEM for scarce and unstable 
membrane protein complexes
Sjors Scheres (MRC-LMB) - Developments in RELION-3

David Bhella (University of Glasgow) - The Scottish Centre for Macromolecular 
Imaging - early adopters of the JEOL CryoARM300
Thomas Braun (University of Basel) - TBC
Doryen Bubeck (Imperial College London) - CryoEM of the membrane attack complex 
reveals how proteins cross lipid bilayers
Felix Weis (EMBL) - How to get the maximum out of your cryo-EM data collection 
session
Wanda Kukulski (MRC-LMB) - Cellular membrane architectures studied by 
correlative microscopy and electron cryo-tomography
Stephen Muench (University of Leeds) - New approaches to grid preparation in 
cryo Electron Microscopy
Stefan Pfeffer (University of Heidelberg) - Structural insights into the 
co-translational machinery for protein folding and maturation
Shaun Rawson (Harvard Medical School) - Rapid evaluation of EM datasets
Sonja Welsch (Thermo Fisher Scientific) - News on Thermo Fisher Scientific’s 
2019 developments for cryoTEM
Pamela Williams (Astex Pharmaceuticals) - TBC

Michele Darrow (TTP Labtech) - Chameleon: Next Generation Sample Preparation 
for CryoEM based on Spotiton
Giulia Weissenberger (CryoSol) - VitroJet: Next Generation Sample Preparation 
for cryo-EM

Plus: what's new at CCP-EM, EBI and eBIC, and selected talks from submitted 
abstracts.

Scientific organisers:
John Briggs (MRC-LMB)
Rebecca Thompson (University of Leeds)

Registration site:
http://www.cvent.com/d/xbqsqk

Registration fees:
PI/Industrial: £225.00
Student/PostDoc: £175.00

We have also ensured reasonably priced accommodation is available from 
Nottingham University (£60/room/night; available on registration).

We hope to see you there!

Best wishes,

Tom, Colin and the rest of the CCP-EM team




To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1

[ccp4bb] Postdoctoral fellowships to study Type 4 Secretion System at Umeå University, Sweden

[Ronnie Berntsson]

Dear all,

The Berntsson lab at the department of Medical Biochemistry and Biophysics at 
Umeå University, Sweden, is looking to recruit up to two postdoctoral fellows 
(2-year fellowships, funded by the Kempe Foundation) to study the structural 
and functional aspects of Type 4 Secretion Systems. These large protein 
complexes are responsible for horizontal gene transfer between bacteria. As 
such, they facilitate the spread of, among other genes, antibiotic resistance 
between bacteria, both intra- and interspecies.

The overall goals of these projects are to structurally and functionally 
characterize the DNA processing proteins of G+ Type 4 Secretion Systems. Parts 
of this project, for work on the T4SS from the pCF10 plasmid from Enterococcus 
faecalis, has already been started in the lab, but we are now also planning on 
expanding to other model systems. The projects will involve molecular biology, 
biochemistry and structural biology techniques, with an early emphasis on the 
functional characterization of these proteins and their complexes, followed by 
structural determination.

Qualifications
The applicants must possess a PhD, or another diploma deemed equivalent to a 
PhD, within molecular biology, biochemistry, structural biology or a related 
field. Furthermore, the applicant must have practical experience and expertise 
of cloning and protein production and purification in bacteria. The applicant 
should preferably be experienced with functional studies of (membrane) proteins 
using various molecular biology and biochemical methods, such as ITC, SPR and 
fluorescence spectroscopy. Previous experience of working with gram-positive 
bacteria and membrane proteins is an advantage, as is previous experience of 
X-ray crystallography and electron microscopy. The applicants must have a very 
good level of English, both written and spoken. The applicants are also 
expected to be good team players, but should also be able to work independently.

Application
The application should consist of the following:

  1.  A motivation letter (max 1 A4), where you highlight why you want to join 
the lab and study T4SSs. This letter must also include your contact information.
  2.  The Curriculum Vitae of the applicant, including a list of published 
peer-reviewed articles.
  3.  Copies of the PhD diploma (or equivalent).
  4.  Contact details for three references, of which one should be your PhD 
supervisor.

The Berntsson lab is part of the Integrated Structural Biology community at 
Umeå University, consisting of 14 research groups that use, and have access to, 
high-field NMR, X-ray crystallography (including regular synchrotron access), 
world-class cryoEM and computation biology with a high performance computing 
centre.

The duration of the fellowship is 2 years. Your application should be written 
in English and prepared as a single package in PDF format, to be submitted to 
ronnie.bernts...@umu.se. Make sure to use the 
subject line: “postdoc application 2019-04” in the application email. Submit 
the application on April 25, 2019 at the latest. The top ranked candidates will 
be contacted within three weeks from the closing date for an interview. 
Starting date according to agreement. For more information about the research 
or other details, do not hesitate to contact Ronnie Berntsson.

Email: ronnie.bernts...@umu.se
Webpage: https://www.biostruct.umu.se/principal-investigators/ronnie-berntsson/

--
Ronnie Berntsson, PhD
Associate Senior Lecturer
Wallenberg Molecular Medicine Fellow
Department of Medical Biochemistry and Biophysics
Umeå University
90187 Umeå
Sweden

e-mail: ronnie.bernts...@umu.se
phone: +46 90 7865235
web: https://www.biostruct.umu.se/principal-investigators/ronnie-berntsson/








To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1

Re: [ccp4bb] Some kind of bug, Can't perform invert selection through file menu in subset selection for micrographs

[Tom Burnley - UKRI STFC]

Dear Suchi,


For questions on Relion (and other EM related things) best to try the CCP-EM 
mailing list (and whilst your reposting I'll try and reproduce your bug on my 
mac ):


https://www.jiscmail.ac.uk/CCPEM

Best wishes,


Tom


From: CCP4 bulletin board  on behalf of Suchi Goel 

Sent: 27 March 2019 02:49:24
To: ccp4bb
Subject: [ccp4bb] Some kind of bug, Can't perform invert selection through file 
menu in subset selection for micrographs

[Screen Shot 2019-03-27 at 11.45.21.png]
[Screen Shot 2019-03-27 at 11.42.19.png]Hi Everyone,

I work on imac and I am kind of stuck at the stage where I want to optimize FOM 
for autopicking. I tried to select subset of micrographs as manual suggested 
but the file tab does not show invert selection to only select a few later. I 
can't unselect all the micrographs already ticked for selection. Could someone 
suggest any fix to that. Pics attached above.

Best Regards,
Suchi Goel



To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1



To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1