[ccp4bb] Postdoctoral fellow position (Houston, TX)

[Zheng, Lei]

A NIH-funded postdoctoral position is available immediately in the Department 
of Biochemistry and Molecular Biology at University of Texas McGovern Medical 
School in the laboratory of Dr. Lei Zheng to study the structure of a membrane 
phosphatase protein and its novel regulatory mechanism. The project involves 
X-ray crystallography, single-particle cryo-electron microscopy, and 
fluorescence spectroscopy.

The ideal candidate should have a recent Ph.D. in Structural Biology, 
Biochemistry or Microbiology with demonstrated experience in protein 
expression, purification and characterization. Experience with membrane protein 
will be a great asset.

The laboratory is located in Texas Medical Center, the largest campus for 
medical research in the world, which provides outstanding environments and 
opportunities for academic development.  Houston is a highly 
culture-diversified city with affordable living costs. Salary will be based on 
NIH standard and individual experience.
Interested applicants should send their CV and the names of three references to 
Dr. Lei Zheng (lei.zh...@uth.tmc,edu).





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[ccp4bb] Searching similar local structural conformations

[Zheng, Lei]

Dear CCP4ers,

We identify a potential ion binding site formed by four residues in a 
structure. I want to search if any other structures have a similar local 
residual geometry. Is there any programs to perform such a searching? For 
example, to search in Protein Data Bank using coordinates of the binding site 
residues? I appreciate your suggestions.

Happy New Year!
Lei





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[ccp4bb] Postdoc position to study membrane protein structure

[Zheng, Lei]

Postdoctoral Position at University of Texas McGovern Medical School at Houston
A postdoctoral position is available in the Department of Biochemistry and 
Molecular Biology at University of Texas McGovern Medical School in the 
laboratory of Dr. Lei Zheng to study the structure and regulatory mechanism of 
mammalian sodium/calcium exchanger proteins using X-ray crystallography and 
cryo-electron microscopy.
Sodium/calcium exchanger proteins are involved in maintaining Ca2+ homeostasis 
in most mammalian tissues and play essential roles in cardiac 
contraction-relaxation and neuronal transmission.  The successful candidate 
will perform protein expression and purification using insect cell systems, and 
use cryo-electron microscopy and single particle analysis approaches to study 
the structure of exchanger complex with small drug inhibitors. The fellow will 
also use X-ray crystallography and other biochemical approaches to study a 
novel regulatory mechanism of exchangers mediated by accessory proteins newly 
identified in our lab.
The ideal candidate should have a recent Ph.D. in Structural Biology and 
Biochemistry with demonstrated experience in protein expression, purification 
and characterization. Experience with membrane protein and insect cell culture 
will be a great asset.  The fellow will have an opportunity to work other 
groups in the department to learn cryo-Electron Microcopy.

The laboratory is located in Texas Medical Center, the largest campus for 
medical research in the world, which provides outstanding environments for 
academic development.  Houston is a highly culture-diversified city with 
affordable living costs. Salary will be based on NIH standard and individual 
experience.

Interested applicants should send their CV and three letters of reference to 
Dr. Lei Zheng (lei.zh...@uth.tmc,edu).


Lei Zheng, Ph.D.
Associate Professor
Center for Membrane Biology
Department of Biochemistry and Molecular Biology
University of Texas McGovern Medical School
6431 Fannin Street, MSB 6.218 (office), 6.408 (lab)
Houston, TX 77030
Tel: 713-500-6083 (office), -7451 (lab)



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[ccp4bb] Postdoc position to study membrane protein structure

[Zheng, Lei]

The position

A NIH-funded postdoctoral position is immediately available in the Center for 
Membrane Biology, Department of Biochemistry and Molecular Biology, University 
of Texas McGovern Medical School at Houston 
(https://med.uth.edu/bmb/research-centers/center-for-membrane-biology/). In 
collaboration with the group of Dr. John Spudich, the fellow will work in the 
lab of Dr. Lei Zheng to study the structure of channelrhodopsin proteins using 
X-ray crystallography and cryo-EM approaches. Channelrhodopsin proteins have 
become extremely powerful tools in optogenetic studies. The candidate will have 
an opportunity to work with new channelrhodopsin proteins recently identified 
in Dr. Spudich’s lab to study the molecular mechanisms of their novel ion 
conductivity and light-induced channel gating. See below link for project info: 
https://www.ncbi.nlm.nih.gov/pubmed/26113638 . The lab is located in the Texas 
Medical Center, the largest medical campus in the world, with great resources 
and environment for biomedical research. Houston is the 4th populous city in 
the United States and is highly international and dynamic.

The candidate

Qualified candidates should have a recent Ph.D. degree and a solid and 
documented background in structural biology, biochemistry and/or biophysics. 
Experience with membrane protein is a big advantage.  A successful candidate is 
expected to be highly skilled and self-motivated and is willing to work in a 
collaborative environment.

Interested candidates should send a CV, one page research experience summary 
and contact information for three references to lei.zh...@uth.tmc.edu. Salary 
will compensate based on NIH standard and candidate’s experience. Starting date 
is negotiable.




Lei Zheng, Ph.D.
Associate Professor
Center for Membrane Biology
Department of Biochemistry and Molecular Biology
University of Texas McGovern Medical School
6431 Fannin Street, MSB 6.218 (office), 6.408 (lab)
Houston, TX 77030
Tel: 713-500-6083 (office), -7451 (lab)

[ccp4bb] A postdoctoral position in University of Texas Houston Medical School

[Zheng, Lei]

The position

A NIH-funded postdoctoral position is immediately available in Center for 
Membrane Biology, Department of Biochemistry and Molecular Biology, University 
of Texas McGovern Medical School at Houston ( 
https://med.uth.edu/bmb/research-centers/center-for-membrane-biology/  ). In 
collaboration with Dr. John Spudich’s group, the researcher will work in the 
lab of Dr. Lei Zheng to study the structure of channel rhodopsin proteins using 
X-ray crystallography and cryo-electron microscopy approaches. See the link 
paper for the project info https://www.ncbi.nlm.nih.gov/pubmed/26113638

The candidate

Qualified candidates should have a recent Ph.D. degree and a solid and 
documented background in structural biology, biochemistry and/or biophysics. 
Candidates with extensive experience of membrane protein expression, 
purification are highly encouraged to apply. He/she also need to be 
self-motivated and is willing to work in a collaborative environment.

Interested candidates should send a CV, one page research experience summary 
and contact information for three references to 
lei.zh...@uth.tmc.edu . Salary will compensate 
based on NIH standard and candidate’s experience. Starting date is negotiable.



Lei Zheng, Ph.D.
Associate Professor
Center for Membrane Biology
Department of Biochemistry and Molecular Biology
University of Texas McGovern Medical School
6431 Fannin Street, MSB 6.218 (office), 6.408 (lab)
Houston, TX 77030
Tel: 713-500-6083 (office), -7451 (lab)

[ccp4bb] Postdoctoral position

[Zheng, Lei]

A postdoctoral position is available immediately in the University of Texas 
Houston Medical School, Department of Biochemistry and Molecular Biology, 
Center for Membrane Biology. The successful applicant will focus on structural 
determination of membrane transporter protein using X-ray crystallography and 
cryo-electron microscopy. The ideal candidate is expected to have a recent PhD 
in structural biology with demonstrated records of structural determination 
using crystallization or single particle analysis techniques. He/she should 
have experience on protein expression and purification and standard molecular 
biology techniques.  Experience with insect cell or mammalian cell cultures is 
a plus.  To apply, please send their CV and names of three references to Lei 
Zheng (lei.zh...@uth.tmc.edu)

[ccp4bb] Postdoc position

[Zheng, Lei]

One postdoctoral position funded by NIH is available immediately at the Center 
for Membrane Biology, Department of Biochemistry and Molecular Biology, The 
University of Texas Houston Medical School (http://www.uth.tmc.edu/cmb/). The 
fellow will focus on structural determination of membrane proteins as a 
function of membrane phospholipid composition. Specifically, we have 
established that half of the transmembrane domains of lactose permease of E. 
coli are inverted with respect to the plane of the membrane bilayer in cells 
lacking the major phospholipid phosphatidylethanolamine (see Annu Rev 
Biochem.http://www.ncbi.nlm.nih.gov/pubmed/19489728?ordinalpos=1itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum
 78:515-40, 2009 for a review). The funded project is to obtain a 
high-resolution structure of the mis-oriented permease by either 2D or 3D 
crystallography. The ideal candidate should be a self-motivated individual and 
have a recent PhD degree in structural biology or biochemistry with 
demonstrated protein crystallization, structural determination and associated 
software experience. The laboratory is located in the Texas Medical Center 
campus, which provides an excellent environment for academia and research. The 
lab is fully equipped for protein x-ray crystallographic study, including a 
Graphon LCP crystallization robot, a Rigaku rotating anode X-ray home source 
and immediate access to the Berkeley ALS beamline 4.2.2 in the framework of the 
molecular biology consortium. To apply or request further information, please 
directly contact: Dr. William Dowhan (E-mail: 
william.dow...@uth.tmc.edumailto:lei.zh...@uth.tmc.edu). The application 
should include a current resume and the names and addresses of three references.

[ccp4bb] Postdoc position

[Zheng, Lei]

One postdoctoral position funded by NIH is available immediately at the Center 
for Membrane Biology, Department of Biochemistry and Molecular Biology, The 
University of Texas Houston Medical School (http://www.uth.tmc.edu/cmb/). The 
fellow will focus on structural determination of important membrane proteins 
involved in lipid metabolism and signaling. The fellow will also work with 
other lipid experts in the center for structural and functional 
characterization.
The ideal candidate should be a self-motivated individual and have a recent PhD 
degree in structural biology or biochemistry. Decent experiences with standard 
molecular biology technique, protein crystallization and structural 
determination approaches are expected.
The laboratory locates in the Texas Medical Center campus which provides an 
excellent environment for academia and research. The lab is fully equipped for 
protein x-ray crystallographic study, including a Graphon LCP crystallization 
robot, a Rigaku rotating anode X-ray home source and immediate access to the 
Berkeley ALS beamline 4.2.2 in the framework of the molecular biology 
consortium.
To apply or request further information, please contact: Dr. Lei Zheng (E-mail: 
lei.zh...@uth.tmc.edu). The application should include a current resume and the 
names and addresses of three referees.

[ccp4bb] Available postdoctoral position

[Zheng, Lei]

One postdoctoral position is available immediately at the Center for Membrane 
Biology, Department of Biochemistry and Molecular Biology, The University of 
Texas Houston Medical School (http://www.uth.tmc.edu/cmb/). The fellow will 
focus on structural determination of important membrane proteins involved in 
lipid metabolism and signaling. The fellow will also work with other lipid 
experts in the center for structural and functional characterization.

The ideal candidate should be a self-motivated individual and have a recent PhD 
degree in structural biology or biochemistry. Decent experiences with standard 
molecular biology technique, protein crystallization and structural 
determination approaches are expected.

The laboratory locates in the Texas Medical Center campus which provides an 
excellent environment for academia and research. The lab is fully equipped for 
protein x-ray crystallographic study, including a Graphon LCP crystallization 
robot, a Rigaku rotating anode X-ray home source and immediate access to the 
Berkeley ALS beamline 4.2.2 in the framework of the molecular biology 
consortium.

To apply or request further information, please contact: Dr. Lei Zheng (E-mail: 
lei.zh...@uth.tmc.edumailto:lei.zh...@uth.tmc.edu). The application should 
include a current resume and the names and addresses of three referees.

[ccp4bb] Search local structural conformation

[Zheng, Lei]

Hi CCP4ers,

I want to search any local structural similarity in the pdb bank with an enzyme 
binding site (8 residues). Can anyone suggest any tools to do this kind of 
local structural conformation searching?
Thank you,
Lei  

[ccp4bb] UV microscope

[Zheng, Lei]

Hi CCP4ers,

I am looking for an affordable UV microscope (not used for automation).  I 
would appreciate it if you would like to share your experience.

Thank you,
Lei

[ccp4bb] A postdoc position

[Zheng, Lei]

A postdoctoral position to study membrane protein structure

One postdoctoral position is available immediately at the Center for Membrane 
Biology, Department of Biochemistry and Molecular Biology, University of Texas 
Health Science Center at Houston. (http://www.uth.tmc.edu/cmb/)

The successful candidate will focus on a well-established membrane protein 
structural biology project.  You will work on structural determination of an 
important ion transporter protein by x-ray crystallography. The protein 
expression and crystallization methods for this protein have been 
well-developed.  You will be responsible to optimize the current 
crystallization conditions using standard membrane protein techniques and other 
novel optimization approaches developed in the laboratory.  You will also have 
an opportunity to work with other expertise in the center to study its 
functional characterization. (Any request for the project details is welcome.)

The ideal applicant should be a self-motivated individual and have a recent PhD 
degree in structural biology, biochemistry or molecular biology. Strong 
background in protein crystallization and structural determination is expected.

The laboratory locates in Texas Medical Center campus which provides an 
excellent environment for academia and research. Our lab is fully equipped with 
all aspects of molecular biology, protein biochemistry and protein x-ray 
crystallography, including a Rigaku rotating anode X-ray home source and 
immediate access to the Berkeley ALS beamline 4.2.2 in the framework of the 
molecular biology consortium.

To apply, please send a current resume and the names and addresses of three 
referees to: Dr. Lei Zheng, Center for Membrane Biology, University of Texas 
HSC, 6431 Fannin St., Houston, TX 77030, USA. E-mail: lei.zh...@uth.tmc.edu
Thank you!


Lei Zheng, Ph.D
Assistant professor
Center for Membrane Biology
Department of Biochemistry and Molecular Biology
University of Texas Medical School at Houston
6431 Fannin St. MSB 6.218
Houston, TX-77030
Tel.: (713)500-6083
Fax: (713)500-0545

[ccp4bb] Postdoctoral position in The University of Texas at Houston

[Zheng, Lei]

A postdoctoral position in structural determination of the membrane
protein
 
 
One postdoctoral position is available immediately at the Center for
Membrane Biology, Department of Biochemistry and Molecular Biology, The
University of Texas Health Science Center at Houston.
(http://www.uth.tmc.edu/cmb/)
 
The successful candidate will focus on the crystallization and
structural determination of a well-characterized ion transporter
protein. She/He will also have an opportunity to work with other
expertise in the Center to study its functional characterization and
lipid regulation. The ideal applicant should be a highly motivated
individual and have a recent PhD degree in structural biology,
biochemistry or molecular biology. Strong background in molecular
biology, protein purification and crystallization is expected. Any
experience with membrane protein will be a plus. 
 
Our laboratory locates in Texas Medical Center campus which provides an
excellent environment for academia and research. The lab is fully
equipped with all aspects of molecular biology, protein biochemistry and
protein crystallography. Routine access to ALS beamline 4.2.2 has been
granted by Molecular Biology Consortium, a remote ALS data collection
system installed in the lab enables instant data collection.
 
To apply, please send a current resume and the names and addresses of
three referees to: Dr. Lei Zheng, Center for Membrane Biology, The
University of Texas Medical School at Houston, 6431 Fannin St., Houston,
TX 77030, USA.

 

 

--

 

Lei Zheng, Ph.D.

Assistant Professor 

Center for Membrane Biology

Department of Biochemistry and Molecular Biology

University of Texas Medical School at Houston

6431 Fannin St. MSB 6.130

Houston, TX 77030

Tel: 713-500-6083 (office), -7451 (lab)

Fax: 713-500-0545

[ccp4bb] problem on protein precipitation

[Zheng, Lei]

Hi ccp4ers,

 

Sorry for this out-topic question:

Recently we have a membrane protein expressed, after solubilized with
detergent and purified from IMAC, the protein looks beautiful in SEC.
However, it completely precipitates after the 2-3 days storage in 4
degree. We supplement 2 mM DTT in the new elute from IMAC, the protein
looks happy during weeks at 4 degree. However, it starts to form an
invisible aggregate (verified from SEC) during the protein concentration
by Centricon. I know this is not uncommon problem for both soluble and
membrane proteins and wonder if anyone has any tip and experience to
overcome this problem.

The protein pI is 8.6, buffer used is pH 7.6. Glycerol is always present
during the purification. We do have high salt (500mM) in the buffer.

 

Thank you for you input in advance,

Lei