Re: [ccp4bb] Interesting pattern on a crystallization drop
Thank you all for the interesting comments and information about bubbles
behavior and dancing droplets!
Unfortunately, for now, I won´t be able to further investigate the process (by
reproducing the crystallization setup under a camera as suggested by Philippe
Dumas), but I will keep this in mind.
Meanwhile, I keep trying to get crystals!
All the best,
Beatriz
--
Beatriz Guimarães
Laboratory of Structural Biology and Protein Engineering
Instituto Carlos Chagas - ICC / FIOCRUZ Paraná
Rua Prof. Algacyr Munhoz Mader, 3775 Bloco C
CIC 81350-010
Curitiba - PR, Brasil
Tel.:+55(41)3316-3225/2104-3438
De: CCP4 bulletin board em nome de DUMAS Philippe
(IGBMC)
Enviado: quinta-feira, 28 de março de 2019 12:48
Para: CCP4BB@JISCMAIL.AC.UK
Assunto: Re: [ccp4bb] Interesting pattern on a crystallization drop
Le Jeudi 28 Mars 2019 12:03 CET, jai mohan
<0cab66323371-dmarc-requ...@jiscmail.ac.uk> a écrit:
A friend of mine (Angel Piñeiro) just suggested to me the "Maragoni effect",
which is likely active in the dancing droplets from Stanford and might explain
the "explosion" into separate bubbles
Indeed, one can see on Wikipedia a video illustrating how the contact of fluids
with different surface tensions can have big effects...
> May be, I do co-relate your crystal pic with Manu Prakash at Stanford on his
> work on Dancing Droplets, briefing the surface tension and evaporation ^ the
> rule of two component fluids. # Since your precipitant contain PVP a shape
> controlling agent
> #https://med.stanford.edu/news/all-news/2015/03/researchers-solve-mystery-of-the-dancing-droplets.html
>
> Best wishes
> S.M.Jaimohan PhD
> On Thursday, 28 March, 2019, 1:54:23 pm IST, Sergei Strelkov
> wrote:
>
> #yiv3861306982 #yiv3861306982
> --P{margin-top:0;margin-bottom:0;}#yiv3861306982
> Artem (and Beatriz),
>
>
>
>
>
> Me bad, could have thought about that! I think you are right.
>
>
>
>
> There were initially bubbles in each drop (7 in one case, 4 in the other).
>
> At some point the bubbles exploded (it was an instantaneous process, not just
> shrinking).
>
>
>
>
> Kind regards,
>
> Sergei
>
>
>
>
> Prof. Sergei V. Strelkov Laboratory for Biocrystallography Department of
> Pharmaceutical Sciences, KU Leuven O, Campus Gasthuisberg, Herestraat 49
> bus 822, 3000 Leuven, Belgium Phone: +32 16 33 08 45, mobile: +32 486 29 41
> 32 Lab pages: http://pharm.kuleuven.be/BiocrystallographyFrom: CCP4 bulletin
> board on behalf of Artem Evdokimov
>
> Sent: Thursday, March 28, 2019 1:07
> To: CCP4BB@JISCMAIL.AC.UK
> Subject: Re: [ccp4bb] Interesting pattern on a crystallization drop Neat!
> Looks like multiple adjacent bubbles that were initially touching but
> eventually shrunk down to the central cores - the connectors are protein
> filaments (skin on the bubbles) left over from when bubbles had contact
> points.
> Artem
> On Wed, Mar 27, 2019, 19:39 Marshall, Bevan (Manufacturing, Parkville)
> wrote:
>
>
> Looked up the condition on C6 (https://c6.csiro.au/C6.asp) and that condition
> is found in both Index and JCSG screens as well as Classics II.
>
>
>
>
>
> Bevan Marshall
> Staff Scientist | Collaborative Crystallisation Centre
> Manufacturing
> CSIRO
>
> E bevan.marsh...@csiro.aut +61 3 9662 7492
> 343-351 Royal Parade, Parkville, VIC 3052
> www.csiro.au|https://c3.csiro.au<http://www.csiro.au|https://c3.csiro.au>
> CSIRO acknowledges the Traditional Owners of the lands that we live and work
> on across Australia and pays its respect to Elders past and present.
>
> PLEASE NOTE
> The information contained in this email may be confidential or privileged.
> Any unauthorised use or disclosure is prohibited. If you have received this
> email in error, please delete it immediately and notify the sender by return
> email. Thank you. To the extent permitted by law, CSIRO does not represent,
> warrant and/or guarantee that the integrity of this communication has been
> maintained or that the communication is free of errors, virus, interception
> or interference.
>
> Please consider the environment before printing this email.
>
>
>
> From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK]On Behalf Of LEGRAND
> Pierre
> Sent: Thursday, 28 March 2019 9:13 AM
> To: CCP4BB@JISCMAIL.AC.UK
> Subject: Re: [ccp4bb] Interesting pattern on a crystallization drop
>
>
>
> Dear Beatriz,
>
>
>
> Nice drops :-))
>
> Could it be that there is a reaction going on in these drops ?
>
> The conditions are quite "exotic" with possibilities of coordination or
> oxydoreduction (Co2+/Co3+
Re: [ccp4bb] Interesting pattern on a crystallization drop
Le Jeudi 28 Mars 2019 12:03 CET, jai mohan
<0cab66323371-dmarc-requ...@jiscmail.ac.uk> a écrit:
A friend of mine (Angel Piñeiro) just suggested to me the "Maragoni effect",
which is likely active in the dancing droplets from Stanford and might explain
the "explosion" into separate bubbles
Indeed, one can see on Wikipedia a video illustrating how the contact of fluids
with different surface tensions can have big effects...
> May be, I do co-relate your crystal pic with Manu Prakash at Stanford on his
> work on Dancing Droplets, briefing the surface tension and evaporation ^ the
> rule of two component fluids. # Since your precipitant contain PVP a shape
> controlling agent
> #https://med.stanford.edu/news/all-news/2015/03/researchers-solve-mystery-of-the-dancing-droplets.html
>
> Best wishes
> S.M.Jaimohan PhD
> On Thursday, 28 March, 2019, 1:54:23 pm IST, Sergei Strelkov
> wrote:
>
> #yiv3861306982 #yiv3861306982
> --P{margin-top:0;margin-bottom:0;}#yiv3861306982
> Artem (and Beatriz),
>
>
>
>
>
> Me bad, could have thought about that! I think you are right.
>
>
>
>
> There were initially bubbles in each drop (7 in one case, 4 in the other).
>
> At some point the bubbles exploded (it was an instantaneous process, not just
> shrinking).
>
>
>
>
> Kind regards,
>
> Sergei
>
>
>
>
> Prof. Sergei V. Strelkov Laboratory for Biocrystallography Department of
> Pharmaceutical Sciences, KU Leuven O, Campus Gasthuisberg, Herestraat 49
> bus 822, 3000 Leuven, Belgium Phone: +32 16 33 08 45, mobile: +32 486 29 41
> 32 Lab pages: http://pharm.kuleuven.be/BiocrystallographyFrom: CCP4 bulletin
> board on behalf of Artem Evdokimov
>
> Sent: Thursday, March 28, 2019 1:07
> To: CCP4BB@JISCMAIL.AC.UK
> Subject: Re: [ccp4bb] Interesting pattern on a crystallization drop Neat!
> Looks like multiple adjacent bubbles that were initially touching but
> eventually shrunk down to the central cores - the connectors are protein
> filaments (skin on the bubbles) left over from when bubbles had contact
> points.
> Artem
> On Wed, Mar 27, 2019, 19:39 Marshall, Bevan (Manufacturing, Parkville)
> wrote:
>
>
> Looked up the condition on C6 (https://c6.csiro.au/C6.asp) and that condition
> is found in both Index and JCSG screens as well as Classics II.
>
>
>
>
>
> Bevan Marshall
> Staff Scientist | Collaborative Crystallisation Centre
> Manufacturing
> CSIRO
>
> E bevan.marsh...@csiro.aut +61 3 9662 7492
> 343-351 Royal Parade, Parkville, VIC 3052
> www.csiro.au|https://c3.csiro.au
> CSIRO acknowledges the Traditional Owners of the lands that we live and work
> on across Australia and pays its respect to Elders past and present.
>
> PLEASE NOTE
> The information contained in this email may be confidential or privileged.
> Any unauthorised use or disclosure is prohibited. If you have received this
> email in error, please delete it immediately and notify the sender by return
> email. Thank you. To the extent permitted by law, CSIRO does not represent,
> warrant and/or guarantee that the integrity of this communication has been
> maintained or that the communication is free of errors, virus, interception
> or interference.
>
> Please consider the environment before printing this email.
>
>
>
> From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK]On Behalf Of LEGRAND
> Pierre
> Sent: Thursday, 28 March 2019 9:13 AM
> To: CCP4BB@JISCMAIL.AC.UK
> Subject: Re: [ccp4bb] Interesting pattern on a crystallization drop
>
>
>
> Dear Beatriz,
>
>
>
> Nice drops :-))
>
> Could it be that there is a reaction going on in these drops ?
>
> The conditions are quite "exotic" with possibilities of coordination or
> oxydoreduction (Co2+/Co3+) or polymerization...
>
> Do you have reductants with the protein buffer ?
>
> Is the protein an enzyme or a metalloprotein ?
>
> Just some ideas.
>
>
>
> Best wishes,
>
> Pierre
>
>
>
> De : CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] de la part de Beatriz Gomes
> Guimaraes [beatriz.guimar...@fiocruz.br]
> Envoyé : mercredi 27 mars 2019 19:44
> À : CCP4BB@JISCMAIL.AC.UK
> Objet : [ccp4bb] Interesting pattern on a crystallization drop
>
> Dear all,
>
>
>
> I would like to share with you a surprising pattern I found when examining
> some crystallization plates (attached figures).
>
>
>
> It is less obvious looking the photos, but apparently the "lines" are formed
> by precipitated protein and there are some "bubbles" with small drops
> inside.I wish they were microcrystals bu
Re: [ccp4bb] Interesting pattern on a crystallization drop
May be, I do co-relate your crystal pic with Manu Prakash at Stanford on his
work on Dancing Droplets, briefing the surface tension and evaporation ^ the
rule of two component fluids. # Since your precipitant contain PVP a shape
controlling agent
#https://med.stanford.edu/news/all-news/2015/03/researchers-solve-mystery-of-the-dancing-droplets.html
Best wishes
S.M.Jaimohan PhD
On Thursday, 28 March, 2019, 1:54:23 pm IST, Sergei Strelkov
wrote:
#yiv3861306982 #yiv3861306982 --P{margin-top:0;margin-bottom:0;}#yiv3861306982
Artem (and Beatriz),
Me bad, could have thought about that! I think you are right.
There were initially bubbles in each drop (7 in one case, 4 in the other).
At some point the bubbles exploded (it was an instantaneous process, not just
shrinking).
Kind regards,
Sergei
Prof. Sergei V. Strelkov Laboratory for Biocrystallography Department of
Pharmaceutical Sciences, KU Leuven O, Campus Gasthuisberg, Herestraat 49 bus
822, 3000 Leuven, Belgium Phone: +32 16 33 08 45, mobile: +32 486 29 41 32 Lab
pages: http://pharm.kuleuven.be/BiocrystallographyFrom: CCP4 bulletin board
on behalf of Artem Evdokimov
Sent: Thursday, March 28, 2019 1:07
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Interesting pattern on a crystallization drop Neat!
Looks like multiple adjacent bubbles that were initially touching but
eventually shrunk down to the central cores - the connectors are protein
filaments (skin on the bubbles) left over from when bubbles had contact points.
Artem
On Wed, Mar 27, 2019, 19:39 Marshall, Bevan (Manufacturing, Parkville)
wrote:
Looked up the condition on C6 (https://c6.csiro.au/C6.asp) and that condition
is found in both Index and JCSG screens as well as Classics II.
Bevan Marshall
Staff Scientist | Collaborative Crystallisation Centre
Manufacturing
CSIRO
E bevan.marsh...@csiro.aut +61 3 9662 7492
343-351 Royal Parade, Parkville, VIC 3052
www.csiro.au|https://c3.csiro.au
CSIRO acknowledges the Traditional Owners of the lands that we live and work on
across Australia and pays its respect to Elders past and present.
PLEASE NOTE
The information contained in this email may be confidential or privileged. Any
unauthorised use or disclosure is prohibited. If you have received this email
in error, please delete it immediately and notify the sender by return email.
Thank you. To the extent permitted by law, CSIRO does not represent, warrant
and/or guarantee that the integrity of this communication has been maintained
or that the communication is free of errors, virus, interception or
interference.
Please consider the environment before printing this email.
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK]On Behalf Of LEGRAND
Pierre
Sent: Thursday, 28 March 2019 9:13 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Interesting pattern on a crystallization drop
Dear Beatriz,
Nice drops :-))
Could it be that there is a reaction going on in these drops ?
The conditions are quite "exotic" with possibilities of coordination or
oxydoreduction (Co2+/Co3+) or polymerization...
Do you have reductants with the protein buffer ?
Is the protein an enzyme or a metalloprotein ?
Just some ideas.
Best wishes,
Pierre
De : CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] de la part de Beatriz Gomes
Guimaraes [beatriz.guimar...@fiocruz.br]
Envoyé : mercredi 27 mars 2019 19:44
À : CCP4BB@JISCMAIL.AC.UK
Objet : [ccp4bb] Interesting pattern on a crystallization drop
Dear all,
I would like to share with you a surprising pattern I found when examining some
crystallization plates (attached figures).
It is less obvious looking the photos, but apparently the "lines" are formed by
precipitated protein and there are some "bubbles" with small drops inside.I
wish they were microcrystals but I do not think this is the case.
I was suprised by the symmetry !
And it is not completely random because for the same condition the difference
between the two drops are : protein alone ("hexagon") and protein + ligand
("rhombus")
crystallization condition is:
0.01 M Cobalt(II) chloride hexahydrate
0.1 M Tris pH 8.5
20% w/v Polyvinylpyrrolidone K 15
Have you seen anything similar before?
Thank you for your comments!
Beatriz
--
Beatriz Guimarães
Laboratory of Structural Biology and Protein Engineering
Instituto Carlos Chagas - ICC / FIOCRUZ Paraná
Rua Prof. Algacyr Munhoz Mader, 3775 Bloco C
CIC 81350-010
Curitiba - PR, Brasil
Tel.:+55(41)3316-3225/2104-3438
To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1
To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1
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Re: [ccp4bb] Interesting pattern on a crystallization drop
Artem (and Beatriz),
Me bad, could have thought about that! I think you are right.
There were initially bubbles in each drop (7 in one case, 4 in the other).
At some point the bubbles exploded (it was an instantaneous process, not just
shrinking).
Kind regards,
Sergei
Prof. Sergei V. Strelkov Laboratory for Biocrystallography Department of
Pharmaceutical Sciences, KU Leuven O, Campus Gasthuisberg, Herestraat 49 bus
822, 3000 Leuven, Belgium Phone: +32 16 33 08 45, mobile: +32 486 29 41 32 Lab
pages:
http://pharm.kuleuven.be/Biocrystallography<http://pharm.kuleuven.be/anafar>
From: CCP4 bulletin board on behalf of Artem Evdokimov
Sent: Thursday, March 28, 2019 1:07
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Interesting pattern on a crystallization drop
Neat!
Looks like multiple adjacent bubbles that were initially touching but
eventually shrunk down to the central cores - the connectors are protein
filaments (skin on the bubbles) left over from when bubbles had contact points.
Artem
On Wed, Mar 27, 2019, 19:39 Marshall, Bevan (Manufacturing, Parkville)
wrote:
Looked up the condition on C6 (https://c6.csiro.au/C6.asp) and that condition
is found in both Index and JCSG screens as well as Classics II.
Bevan Marshall
Staff Scientist | Collaborative Crystallisation Centre
Manufacturing
CSIRO
E bevan.marsh...@csiro.au<mailto:bevan.marsh...@csiro.au>T +61 3 9662 7492
343-351 Royal Parade, Parkville, VIC 3052
www.csiro.au<http://www.csiro.au/> | https://c3.csiro.au<https://c3.csiro.au/>
CSIRO acknowledges the Traditional Owners of the lands that we live and work on
across Australia and pays its respect to Elders past and present.
PLEASE NOTE
The information contained in this email may be confidential or privileged. Any
unauthorised use or disclosure is prohibited. If you have received this email
in error, please delete it immediately and notify the sender by return email.
Thank you. To the extent permitted by law, CSIRO does not represent, warrant
and/or guarantee that the integrity of this communication has been maintained
or that the communication is free of errors, virus, interception or
interference.
Please consider the environment before printing this email.
From: CCP4 bulletin board
[mailto:CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK>] On Behalf Of
LEGRAND Pierre
Sent: Thursday, 28 March 2019 9:13 AM
To: CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK>
Subject: Re: [ccp4bb] Interesting pattern on a crystallization drop
Dear Beatriz,
Nice drops :-))
Could it be that there is a reaction going on in these drops ?
The conditions are quite "exotic" with possibilities of coordination or
oxydoreduction (Co2+/Co3+) or polymerization...
Do you have reductants with the protein buffer ?
Is the protein an enzyme or a metalloprotein ?
Just some ideas.
Best wishes,
Pierre
De : CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK>]
de la part de Beatriz Gomes Guimaraes
[beatriz.guimar...@fiocruz.br<mailto:beatriz.guimar...@fiocruz.br>]
Envoyé : mercredi 27 mars 2019 19:44
À : CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK>
Objet : [ccp4bb] Interesting pattern on a crystallization drop
Dear all,
I would like to share with you a surprising pattern I found when examining some
crystallization plates (attached figures).
It is less obvious looking the photos, but apparently the "lines" are formed by
precipitated protein and there are some "bubbles" with small drops inside. I
wish they were microcrystals but I do not think this is the case.
I was suprised by the symmetry !
And it is not completely random because for the same condition the difference
between the two drops are : protein alone ("hexagon") and protein + ligand
("rhombus")
crystallization condition is:
0.01 M Cobalt(II) chloride hexahydrate
0.1 M Tris pH 8.5
20% w/v Polyvinylpyrrolidone K 15
Have you seen anything similar before?
Thank you for your comments!
Beatriz
--
Beatriz Guimarães
Laboratory of Structural Biology and Protein Engineering
Instituto Carlos Chagas - ICC / FIOCRUZ Paraná
Rua Prof. Algacyr Munhoz Mader, 3775 Bloco C
CIC 81350-010
Curitiba - PR, Brasil
Tel.:+55(41)3316-3225/2104-3438
To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1
To unsubscribe from the CCP4BB list, click the following link:
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To unsubscribe fro
Re: [ccp4bb] Interesting pattern on a crystallization drop
Hi Beatriz,
Very interesting indeed. Couldn't it be the ghost printing of some mechanical
part used to make the mould of the crystallization tray ?
All the best,
Philippe
Philippe BENAS, Ph.D.
ARN UPR 9002 CNRS
IBMC Strasbourg
15, rue René Descartes
F-67084 STRASBOURG cedex
+33.3.8841.7109
E-mails: p.be...@ibmc-cnrs.unistra.fr, philippe_be...@yahoo.fr
URLs: http://www-ibmc.u-strasbg.fr/ , http://www-ibmc.u-strasbg.fr/spip-arn/
Le mercredi 27 mars 2019 à 19:54:42 UTC+1, Beatriz Gomes Guimaraes
a écrit :
Dear all,
I would like to share with you a surprising pattern I found when examining some
crystallization plates (attached figures).
It is less obvious looking the photos, but apparently the "lines" are formed by
precipitated protein and there are some "bubbles" with small drops inside.I
wish they were microcrystals but I do not think this is the case.
I was suprised by the symmetry !
And it is not completely random because for the same condition the difference
between the two drops are : protein alone ("hexagon") and protein + ligand
("rhombus")
crystallization condition is:
0.01 M Cobalt(II) chloride hexahydrate
0.1 M Tris pH 8.5
20% w/v Polyvinylpyrrolidone K 15
Have you seen anything similar before?
Thank you for your comments!
Beatriz
--
Beatriz Guimarães
Laboratory of Structural Biology and Protein Engineering
Instituto Carlos Chagas - ICC / FIOCRUZ Paraná
Rua Prof. Algacyr Munhoz Mader, 3775 Bloco C
CIC 81350-010
Curitiba - PR, Brasil
Tel.:+55(41)3316-3225/2104-3438
To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1
To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1
Re: [ccp4bb] Interesting pattern on a crystallization drop
Neat!
Looks like multiple adjacent bubbles that were initially touching but
eventually shrunk down to the central cores - the connectors are protein
filaments (skin on the bubbles) left over from when bubbles had contact
points.
Artem
On Wed, Mar 27, 2019, 19:39 Marshall, Bevan (Manufacturing, Parkville)
wrote:
> Looked up the condition on C6 (https://c6.csiro.au/C6.asp) and that
> condition is found in both Index and JCSG screens as well as Classics II.
>
>
>
>
>
> *Bevan Marshall*
> Staff Scientist | Collaborative Crystallisation Centre
> *Manufacturing*
> CSIRO
>
> E bevan.marsh...@csiro.aut +61 3 9662 7492
> 343-351 Royal Parade, Parkville, VIC 3052
> www.csiro.au | https://c3.csiro.au
> CSIRO acknowledges the Traditional Owners of the lands that we live and
> work on across Australia and pays its respect to Elders past and present.
>
> *PLEASE NOTE*
> The information contained in this email may be confidential or privileged.
> Any unauthorised use or disclosure is prohibited. If you have received this
> email in error, please delete it immediately and notify the sender by
> return email. Thank you. To the extent permitted by law, CSIRO does not
> represent, warrant and/or guarantee that the integrity of this
> communication has been maintained or that the communication is free of
> errors, virus, interception or interference.
>
> *Please consider the environment before printing this email.*
>
>
>
> *From:* CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] *On Behalf Of
> *LEGRAND
> Pierre
> *Sent:* Thursday, 28 March 2019 9:13 AM
> *To:* CCP4BB@JISCMAIL.AC.UK
> *Subject:* Re: [ccp4bb] Interesting pattern on a crystallization drop
>
>
>
> Dear Beatriz,
>
>
>
> Nice drops :-))
>
> Could it be that there is a reaction going on in these drops ?
>
> The conditions are quite "exotic" with possibilities of coordination or
> oxydoreduction (Co2+/Co3+) or polymerization...
>
> Do you have reductants with the protein buffer ?
>
> Is the protein an enzyme or a metalloprotein ?
>
> Just some ideas.
>
>
>
> Best wishes,
>
> Pierre
>
>
> --
>
> *De :* CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] de la part de Beatriz
> Gomes Guimaraes [beatriz.guimar...@fiocruz.br]
> *Envoyé :* mercredi 27 mars 2019 19:44
> *À :* CCP4BB@JISCMAIL.AC.UK
> *Objet :* [ccp4bb] Interesting pattern on a crystallization drop
>
> Dear all,
>
>
>
> I would like to share with you a surprising pattern I found when examining
> some crystallization plates (attached figures).
>
>
>
> It is less obvious looking the photos, but apparently the "lines" are
> formed by precipitated protein and there are some "bubbles" with small
> drops inside. I wish they were microcrystals but I do not think this is
> the case.
>
> I was suprised by the symmetry !
>
>
>
> And it is not completely random because for the same condition the
> difference between the two drops are : protein alone ("hexagon") and
> protein + ligand ("rhombus")
>
>
>
> crystallization condition is:
>
> 0.01 M Cobalt(II) chloride hexahydrate
>
> 0.1 M Tris pH 8.5
>
> 20% w/v Polyvinylpyrrolidone K 15
>
>
>
> Have you seen anything similar before?
>
>
>
> Thank you for your comments!
>
> Beatriz
>
>
>
>
>
> --
> Beatriz Guimarães
> Laboratory of Structural Biology and Protein Engineering
> Instituto Carlos Chagas - ICC / FIOCRUZ Paraná
> Rua Prof. Algacyr Munhoz Mader, 3775 Bloco C
> CIC 81350-010
> Curitiba - PR, Brasil
> Tel.:+55(41)3316-3225/2104-3438
>
>
> --
>
> To unsubscribe from the CCP4BB list, click the following link:
> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1
>
>
> --
>
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Re: [ccp4bb] Interesting pattern on a crystallization drop
Looked up the condition on C6 (https://c6.csiro.au/C6.asp) and that condition
is found in both Index and JCSG screens as well as Classics II.
Bevan Marshall
Staff Scientist | Collaborative Crystallisation Centre
Manufacturing
CSIRO
E bevan.marsh...@csiro.au<mailto:bevan.marsh...@csiro.au>T +61 3 9662 7492
343-351 Royal Parade, Parkville, VIC 3052
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From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of LEGRAND
Pierre
Sent: Thursday, 28 March 2019 9:13 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Interesting pattern on a crystallization drop
Dear Beatriz,
Nice drops :-))
Could it be that there is a reaction going on in these drops ?
The conditions are quite "exotic" with possibilities of coordination or
oxydoreduction (Co2+/Co3+) or polymerization...
Do you have reductants with the protein buffer ?
Is the protein an enzyme or a metalloprotein ?
Just some ideas.
Best wishes,
Pierre
De : CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] de la part de Beatriz Gomes
Guimaraes [beatriz.guimar...@fiocruz.br]
Envoyé : mercredi 27 mars 2019 19:44
À : CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK>
Objet : [ccp4bb] Interesting pattern on a crystallization drop
Dear all,
I would like to share with you a surprising pattern I found when examining some
crystallization plates (attached figures).
It is less obvious looking the photos, but apparently the "lines" are formed by
precipitated protein and there are some "bubbles" with small drops inside. I
wish they were microcrystals but I do not think this is the case.
I was suprised by the symmetry !
And it is not completely random because for the same condition the difference
between the two drops are : protein alone ("hexagon") and protein + ligand
("rhombus")
crystallization condition is:
0.01 M Cobalt(II) chloride hexahydrate
0.1 M Tris pH 8.5
20% w/v Polyvinylpyrrolidone K 15
Have you seen anything similar before?
Thank you for your comments!
Beatriz
--
Beatriz Guimarães
Laboratory of Structural Biology and Protein Engineering
Instituto Carlos Chagas - ICC / FIOCRUZ Paraná
Rua Prof. Algacyr Munhoz Mader, 3775 Bloco C
CIC 81350-010
Curitiba - PR, Brasil
Tel.:+55(41)3316-3225/2104-3438
To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1
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Re: [ccp4bb] Interesting pattern on a crystallization drop
Dear Beatriz,
Nice drops :-))
Could it be that there is a reaction going on in these drops ?
The conditions are quite "exotic" with possibilities of coordination or
oxydoreduction (Co2+/Co3+) or polymerization...
Do you have reductants with the protein buffer ?
Is the protein an enzyme or a metalloprotein ?
Just some ideas.
Best wishes,
Pierre
De : CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] de la part de Beatriz Gomes
Guimaraes [beatriz.guimar...@fiocruz.br]
Envoyé : mercredi 27 mars 2019 19:44
À : CCP4BB@JISCMAIL.AC.UK
Objet : [ccp4bb] Interesting pattern on a crystallization drop
Dear all,
I would like to share with you a surprising pattern I found when examining some
crystallization plates (attached figures).
It is less obvious looking the photos, but apparently the "lines" are formed by
precipitated protein and there are some "bubbles" with small drops inside. I
wish they were microcrystals but I do not think this is the case.
I was suprised by the symmetry !
And it is not completely random because for the same condition the difference
between the two drops are : protein alone ("hexagon") and protein + ligand
("rhombus")
crystallization condition is:
0.01 M Cobalt(II) chloride hexahydrate
0.1 M Tris pH 8.5
20% w/v Polyvinylpyrrolidone K 15
Have you seen anything similar before?
Thank you for your comments!
Beatriz
--
Beatriz Guimarães
Laboratory of Structural Biology and Protein Engineering
Instituto Carlos Chagas - ICC / FIOCRUZ Paraná
Rua Prof. Algacyr Munhoz Mader, 3775 Bloco C
CIC 81350-010
Curitiba - PR, Brasil
Tel.:+55(41)3316-3225/2104-3438
To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1
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Re: [ccp4bb] Interesting pattern on a crystallization drop
Dear all,
some more details:
drops were set up by a robot (Mosquito), 200 nL prot+ 200 nL reservoir on a
96-Well sitting drop iQ Plate (TTPlabtech). They are not really bubbles. I have
the impression that the lines are all around creating these "empty rounds"
(maybe "exploded bubbles"?) with the small drop inside.
Beatriz
--
Beatriz Guimarães
Laboratory of Structural Biology and Protein Engineering
Instituto Carlos Chagas - ICC / FIOCRUZ Paraná
Rua Prof. Algacyr Munhoz Mader, 3775 Bloco C
CIC 81350-010
Curitiba - PR, Brasil
Tel.:+55(41)3316-3225/2104-3438
De: Ailong Ke
Enviado: quarta-feira, 27 de março de 2019 17:06:01
Para: Beatriz Gomes Guimaraes
Cc: CCP4BB@JISCMAIL.AC.UK
Assunto: Re: [ccp4bb] Interesting pattern on a crystallization drop
Hi Beatriz,
Were the drops set up by hand or by a robot? If the latter, what kind of robot?
are they hanging drops or sitting drops, and do the bubbles form on the glass
surface of at the bottom of your drop?
AK
On Mar 27, 2019, at 2:44 PM, Beatriz Gomes Guimaraes
mailto:beatriz.guimar...@fiocruz.br>> wrote:
Dear all,
I would like to share with you a surprising pattern I found when examining some
crystallization plates (attached figures).
It is less obvious looking the photos, but apparently the "lines" are formed by
precipitated protein and there are some "bubbles" with small drops inside. I
wish they were microcrystals but I do not think this is the case.
I was suprised by the symmetry !
And it is not completely random because for the same condition the difference
between the two drops are : protein alone ("hexagon") and protein + ligand
("rhombus")
crystallization condition is:
0.01 M Cobalt(II) chloride hexahydrate
0.1 M Tris pH 8.5
20% w/v Polyvinylpyrrolidone K 15
Have you seen anything similar before?
Thank you for your comments!
Beatriz
--
Beatriz Guimarães
Laboratory of Structural Biology and Protein Engineering
Instituto Carlos Chagas - ICC / FIOCRUZ Paraná
Rua Prof. Algacyr Munhoz Mader, 3775 Bloco C
CIC 81350-010
Curitiba - PR, Brasil
Tel.:+55(41)3316-3225/2104-3438
To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1
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Re: [ccp4bb] Interesting pattern on a crystallization drop
Hi Beatriz,
Were the drops set up by hand or by a robot? If the latter, what kind of robot?
are they hanging drops or sitting drops, and do the bubbles form on the glass
surface of at the bottom of your drop?
AK
On Mar 27, 2019, at 2:44 PM, Beatriz Gomes Guimaraes
mailto:beatriz.guimar...@fiocruz.br>> wrote:
Dear all,
I would like to share with you a surprising pattern I found when examining some
crystallization plates (attached figures).
It is less obvious looking the photos, but apparently the "lines" are formed by
precipitated protein and there are some "bubbles" with small drops inside. I
wish they were microcrystals but I do not think this is the case.
I was suprised by the symmetry !
And it is not completely random because for the same condition the difference
between the two drops are : protein alone ("hexagon") and protein + ligand
("rhombus")
crystallization condition is:
0.01 M Cobalt(II) chloride hexahydrate
0.1 M Tris pH 8.5
20% w/v Polyvinylpyrrolidone K 15
Have you seen anything similar before?
Thank you for your comments!
Beatriz
--
Beatriz Guimarães
Laboratory of Structural Biology and Protein Engineering
Instituto Carlos Chagas - ICC / FIOCRUZ Paraná
Rua Prof. Algacyr Munhoz Mader, 3775 Bloco C
CIC 81350-010
Curitiba - PR, Brasil
Tel.:+55(41)3316-3225/2104-3438
To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1
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Re: [ccp4bb] Interesting pattern on a crystallization drop
It's a crystal with a very large lattice.
JPK
+
Jacob Pearson Keller
Research Scientist / Looger Lab
HHMI Janelia Research Campus
19700 Helix Dr, Ashburn, VA 20147
Desk: (571)209-4000 x3159
Cell: (301)592-7004
+
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From: CCP4 bulletin board On Behalf Of Beatriz Gomes
Guimaraes
Sent: Wednesday, March 27, 2019 2:44 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Interesting pattern on a crystallization drop
Dear all,
I would like to share with you a surprising pattern I found when examining some
crystallization plates (attached figures).
It is less obvious looking the photos, but apparently the "lines" are formed by
precipitated protein and there are some "bubbles" with small drops inside. I
wish they were microcrystals but I do not think this is the case.
I was suprised by the symmetry !
And it is not completely random because for the same condition the difference
between the two drops are : protein alone ("hexagon") and protein + ligand
("rhombus")
crystallization condition is:
0.01 M Cobalt(II) chloride hexahydrate
0.1 M Tris pH 8.5
20% w/v Polyvinylpyrrolidone K 15
Have you seen anything similar before?
Thank you for your comments!
Beatriz
--
Beatriz Guimarães
Laboratory of Structural Biology and Protein Engineering
Instituto Carlos Chagas - ICC / FIOCRUZ Paraná
Rua Prof. Algacyr Munhoz Mader, 3775 Bloco C
CIC 81350-010
Curitiba - PR, Brasil
Tel.:+55(41)3316-3225/2104-3438
To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1
To unsubscribe from the CCP4BB list, click the following link:
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Re: [ccp4bb] Interesting pattern on a crystallization drop
I'm guessing quite big drops on a somewhat hydrophobic surface? Also guessing
that the protein foams quite a bit, and that there were 6 (or four) more or
less equal size bubbles that took up almost the whole drop when it was set up.
Bubbles tend to pack quite efficiently
(https://physicsworld.com/a/getting-to-the-bottom-of-foamy-physics/), giving
the base symmetry that stayed when the bubbles burst. Wondering if the lines
are from the collapse of the thin protein/polymer skin covering the surface of
the bubbles?
lovely and thought provoking images - thanks!
Janet
[https://physicsworld.com/wp-content/uploads/2013/05/bubbles.jpg]<https://physicsworld.com/a/getting-to-the-bottom-of-foamy-physics/>
Getting to the bottom of foamy physics – Physics
World<https://physicsworld.com/a/getting-to-the-bottom-of-foamy-physics/>
physicsworld.com
Researchers in the US have created a new mathematical model to describe the
complex evolution of foamy bubbles – something that has proved fiendishly
difficult to model thanks to the hugely varying length and time scales
involved. Their computed results closely match theoretical models as well as ...
Janet Newman
Principal Scientist / Director, Collaborative Crystallisation Centre (C3)
CSIRO Material Science and Engineering
343 Royal Parade
Parkville. VIC. 3052
Australia
Tel +613 9662 7326
Email janet.new...@csiro.au
From: CCP4 bulletin board on behalf of Beatriz Gomes
Guimaraes
Sent: Thursday, March 28, 2019 5:44 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Interesting pattern on a crystallization drop
Dear all,
I would like to share with you a surprising pattern I found when examining some
crystallization plates (attached figures).
It is less obvious looking the photos, but apparently the "lines" are formed by
precipitated protein and there are some "bubbles" with small drops inside. I
wish they were microcrystals but I do not think this is the case.
I was suprised by the symmetry !
And it is not completely random because for the same condition the difference
between the two drops are : protein alone ("hexagon") and protein + ligand
("rhombus")
crystallization condition is:
0.01 M Cobalt(II) chloride hexahydrate
0.1 M Tris pH 8.5
20% w/v Polyvinylpyrrolidone K 15
Have you seen anything similar before?
Thank you for your comments!
Beatriz
--
Beatriz Guimarães
Laboratory of Structural Biology and Protein Engineering
Instituto Carlos Chagas - ICC / FIOCRUZ Paraná
Rua Prof. Algacyr Munhoz Mader, 3775 Bloco C
CIC 81350-010
Curitiba - PR, Brasil
Tel.:+55(41)3316-3225/2104-3438
To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1
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Re: [ccp4bb] Interesting pattern on a crystallization drop
Hi Philippe
I do not know yet if it is reproducible. And protein function has nothing to do
with polymerization or something ... it is a RNA interacting protein, small and
usually monomeric.
I will try to find a better one for the April 1st... but it will be hard!
Best regards,
Beatriz
--
Beatriz Guimarães
Laboratory of Structural Biology and Protein Engineering
Instituto Carlos Chagas - ICC / FIOCRUZ Paraná
Rua Prof. Algacyr Munhoz Mader, 3775 Bloco C
CIC 81350-010
Curitiba - PR, Brasil
Tel.:+55(41)3316-3225/2104-3438
De: DUMAS Philippe (IGBMC)
Enviado: quarta-feira, 27 de março de 2019 16:34
Para: Beatriz Gomes Guimaraes
Cc: CCP4BB@JISCMAIL.AC.UK
Assunto: Re: [ccp4bb] Interesting pattern on a crystallization drop
Le Mercredi 27 Mars 2019 19:44 CET, Beatriz Gomes Guimaraes
a écrit:
In four days from now, I (everyone) would have taken this as a Photoshop joke...
Quite amazing! Is it reproducible?
What is the protein? Tubulin ? ::))
Philippe Dumas
> Dear all,
>
>
> I would like to share with you a surprising pattern I found when examining
> some crystallization plates (attached figures).
>
>
> It is less obvious looking the photos, but apparently the "lines" are formed
> by precipitated protein and there are some "bubbles" with small drops inside.
> I wish they were microcrystals but I do not think this is the case.
>
> I was suprised by the symmetry !
>
>
> And it is not completely random because for the same condition the difference
> between the two drops are : protein alone ("hexagon") and protein + ligand
> ("rhombus")
>
>
> crystallization condition is:
>
> 0.01 M Cobalt(II) chloride hexahydrate
>
> 0.1 M Tris pH 8.5
>
> 20% w/v Polyvinylpyrrolidone K 15
>
>
> Have you seen anything similar before?
>
>
> Thank you for your comments!
>
> Beatriz
>
>
>
> --
> Beatriz Guimarães
> Laboratory of Structural Biology and Protein Engineering
> Instituto Carlos Chagas - ICC / FIOCRUZ Paraná
> Rua Prof. Algacyr Munhoz Mader, 3775 Bloco C
> CIC 81350-010
> Curitiba - PR, Brasil
> Tel.:+55(41)3316-3225/2104-3438
>
>
>
> To unsubscribe from the CCP4BB list, click the following link:
> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1
To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1
Re: [ccp4bb] Interesting pattern on a crystallization drop
Le Mercredi 27 Mars 2019 19:44 CET, Beatriz Gomes Guimaraes
a écrit:
In four days from now, I (everyone) would have taken this as a Photoshop joke...
Quite amazing! Is it reproducible?
What is the protein? Tubulin ? ::))
Philippe Dumas
> Dear all,
>
>
> I would like to share with you a surprising pattern I found when examining
> some crystallization plates (attached figures).
>
>
> It is less obvious looking the photos, but apparently the "lines" are formed
> by precipitated protein and there are some "bubbles" with small drops inside.
> I wish they were microcrystals but I do not think this is the case.
>
> I was suprised by the symmetry !
>
>
> And it is not completely random because for the same condition the difference
> between the two drops are : protein alone ("hexagon") and protein + ligand
> ("rhombus")
>
>
> crystallization condition is:
>
> 0.01 M Cobalt(II) chloride hexahydrate
>
> 0.1 M Tris pH 8.5
>
> 20% w/v Polyvinylpyrrolidone K 15
>
>
> Have you seen anything similar before?
>
>
> Thank you for your comments!
>
> Beatriz
>
>
>
> --
> Beatriz Guimarães
> Laboratory of Structural Biology and Protein Engineering
> Instituto Carlos Chagas - ICC / FIOCRUZ Paraná
> Rua Prof. Algacyr Munhoz Mader, 3775 Bloco C
> CIC 81350-010
> Curitiba - PR, Brasil
> Tel.:+55(41)3316-3225/2104-3438
>
>
>
> To unsubscribe from the CCP4BB list, click the following link:
> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1
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